schliessen

Filtern

 

Bibliotheken

Identification of Cytolytic CD161\(^−\)CD56\(^+\) Regulatory CD8 T Cells in Human Peripheral Blood

We previously developed methods for establishing CD8 regulatory T cell (Treg) clones from normal human peripheral blood and demonstrated that these clones were capable of killing T cell receptor (TCR)-activated autologous CD4 T cells. Based on phenotypic and functional characterization of the CD8 Tr... Full description

Journal Title: Hu Dan, Howard L. Weiner, and Jerome Ritz. 2013. Identification of cytolytic CD161\(^−\)CD56\(^+\) regulatory CD8 cells in human peripheral blood. PLoS ONE 8(3): e59545.
Main Author: Hu, Dan
Other Authors: Weiner, Howard L. , Ritz, Jerome
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0059545
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: dash1/10591654
title: Identification of Cytolytic CD161\(^−\)CD56\(^+\) Regulatory CD8 T Cells in Human Peripheral Blood
format: Article
creator:
  • Hu, Dan
  • Weiner, Howard L.
  • Ritz, Jerome
subjects:
  • Biology
  • Immunology
  • Immune Cells
  • T Cells
  • Immunity
  • Immune Suppression
  • Immune Tolerance
  • Immunoregulation
  • Immune Response
  • Immune System
  • Immunomodulation
  • Medicine
  • Anatomy And Physiology
  • Immune Physiology
  • Cell Surface Molecules
  • Clinical Immunology
ispartof: Hu, Dan, Howard L. Weiner, and Jerome Ritz. 2013. Identification of cytolytic CD161\(^−\)CD56\(^+\) regulatory CD8 cells in human peripheral blood. PLoS ONE 8(3): e59545.
description: We previously developed methods for establishing CD8 regulatory T cell (Treg) clones from normal human peripheral blood and demonstrated that these clones were capable of killing T cell receptor (TCR)-activated autologous CD4 T cells. Based on phenotypic and functional characterization of the CD8 Treg clones, we have identified a corresponding population of endogenous CD8 Treg in normal human peripheral blood. These cells appear morphologically as large lymphocytes with abundant cytoplasm and have the following unique phenotype: CD3\(^+\)CD8\(^+\)CD161\(^−\)CD56\(^+\). The majority of CD8 Treg express CD45RA and CD62L with low or negative expression of CD45RO, CD25, CD27, CD28 and CCR7. The expression of CD94 and NKG2a on CD8 Treg was elevated compared to conventional CD8 T cells. Following in vitro activation, this T cell subset is capable of killing TCR-activated CD4 T cells. These studies identify an endogenous CD8 Treg population in humans and it will now be possible to characterize these cells in a variety of clinical conditions.
language: eng
source:
identifier: ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0059545
fulltext: fulltext_linktorsrc
issn:
  • 1932-6203
  • 19326203
url: Link


@attributes
ID370547715
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid1/10591654
sourceiddash
recordidTN_dash1/10591654
sourcesystemPC
pqid1330893378
galeid478207767
display
typearticle
titleIdentification of Cytolytic CD161\(^−\)CD56\(^+\) Regulatory CD8 T Cells in Human Peripheral Blood
creatorHu, Dan ; Weiner, Howard L. ; Ritz, Jerome
ispartofHu, Dan, Howard L. Weiner, and Jerome Ritz. 2013. Identification of cytolytic CD161\(^−\)CD56\(^+\) regulatory CD8 cells in human peripheral blood. PLoS ONE 8(3): e59545.
identifierISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0059545
subjectBiology ; Immunology ; Immune Cells ; T Cells ; Immunity ; Immune Suppression ; Immune Tolerance ; Immunoregulation ; Immune Response ; Immune System ; Immunomodulation ; Medicine ; Anatomy And Physiology ; Immune Physiology ; Cell Surface Molecules ; Clinical Immunology
descriptionWe previously developed methods for establishing CD8 regulatory T cell (Treg) clones from normal human peripheral blood and demonstrated that these clones were capable of killing T cell receptor (TCR)-activated autologous CD4 T cells. Based on phenotypic and functional characterization of the CD8 Treg clones, we have identified a corresponding population of endogenous CD8 Treg in normal human peripheral blood. These cells appear morphologically as large lymphocytes with abundant cytoplasm and have the following unique phenotype: CD3\(^+\)CD8\(^+\)CD161\(^−\)CD56\(^+\). The majority of CD8 Treg express CD45RA and CD62L with low or negative expression of CD45RO, CD25, CD27, CD28 and CCR7. The expression of CD94 and NKG2a on CD8 Treg was elevated compared to conventional CD8 T cells. Following in vitro activation, this T cell subset is capable of killing TCR-activated CD4 T cells. These studies identify an endogenous CD8 Treg population in humans and it will now be possible to characterize these cells in a variety of clinical conditions.
languageeng
source
version10
oafree_for_read
lds50peer_reviewed
links
openurl$$Topenurl_article
backlink
0$$Uhttp://dx.doi.org/10.1371/journal.pone.0059545$$EView_record
1$$Uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3602421/pdf/$$EView_record
linktorsrc$$Uhttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10591654$$EView_full_text_in_Digital_Access_to_Scholarship_at_Harvard_(DASH)
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0Hu, Dan
1Weiner, Howard L.
2Ritz, Jerome
titleIdentification of Cytolytic CD161\(^−\)CD56\(^+\) Regulatory CD8 T Cells in Human Peripheral Blood
descriptionWe previously developed methods for establishing CD8 regulatory T cell (Treg) clones from normal human peripheral blood and demonstrated that these clones were capable of killing T cell receptor (TCR)-activated autologous CD4 T cells. Based on phenotypic and functional characterization of the CD8 Treg clones, we have identified a corresponding population of endogenous CD8 Treg in normal human peripheral blood. These cells appear morphologically as large lymphocytes with abundant cytoplasm and have the following unique phenotype: CD3\(^+\)CD8\(^+\)CD161\(^−\)CD56\(^+\). The majority of CD8 Treg express CD45RA and CD62L with low or negative expression of CD45RO, CD25, CD27, CD28 and CCR7. The expression of CD94 and NKG2a on CD8 Treg was elevated compared to conventional CD8 T cells. Following in vitro activation, this T cell subset is capable of killing TCR-activated CD4 T cells. These studies identify an endogenous CD8 Treg population in humans and it will now be possible to characterize these cells in a variety of clinical conditions.
subject
0Biology
1Immunology
2Immune Cells
3T Cells
4Immunity
5Immune Suppression
6Immune Tolerance
7Immunoregulation
8Immune Response
9Immune System
10Immunomodulation
11Medicine
12Anatomy and Physiology
13Immune Physiology
14Cell Surface Molecules
15Clinical Immunology
general
0Digital Access to Scholarship at Harvard (DASH)
1Public Library of Science
2English
310.1371/journal.pone.0059545
sourceiddash
recordiddash1/10591654
issn
01932-6203
119326203
rsrctypearticle
creationdate2013
searchscopedash
scopedash
lsr30VSR-Enriched:[pages, vol, galeid, issue, pqid]
sort
titleIdentification of Cytolytic CD161\(^−\)CD56\(^+\) Regulatory CD8 T Cells in Human Peripheral Blood
authorHu, Dan ; Weiner, Howard L. ; Ritz, Jerome
creationdate20130000
facets
frbrgroupid2085842219439981014
frbrtype5
languageeng
creationdate2013
topic
0Biology
1Immunology
2Immune Cells
3T Cells
4Immunity
5Immune Suppression
6Immune Tolerance
7Immunoregulation
8Immune Response
9Immune System
10Immunomodulation
11Medicine
12Anatomy And Physiology
13Immune Physiology
14Cell Surface Molecules
15Clinical Immunology
collectionDASH (Harvard Library)
prefilterarticles
rsrctypearticles
creatorcontrib
0Hu, Dan
1Weiner, Howard L
2Ritz, Jerome
jtitlePLoS ONE
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext_linktorsrc
addata
aulast
0Hu
1Weiner
2Ritz
aufirst
0Dan
1Howard L.
2Jerome
au
0Hu, Dan
1Weiner, Howard L.
2Ritz, Jerome
atitleIdentification of Cytolytic CD161\(^−\)CD56\(^+\) Regulatory CD8 T Cells in Human Peripheral Blood
jtitlePLoS ONE
date2013
risdate2013
issn1932-6203
genrearticle
ristypeJOUR
abstractWe previously developed methods for establishing CD8 regulatory T cell (Treg) clones from normal human peripheral blood and demonstrated that these clones were capable of killing T cell receptor (TCR)-activated autologous CD4 T cells. Based on phenotypic and functional characterization of the CD8 Treg clones, we have identified a corresponding population of endogenous CD8 Treg in normal human peripheral blood. These cells appear morphologically as large lymphocytes with abundant cytoplasm and have the following unique phenotype: CD3\(^+\)CD8\(^+\)CD161\(^−\)CD56\(^+\). The majority of CD8 Treg express CD45RA and CD62L with low or negative expression of CD45RO, CD25, CD27, CD28 and CCR7. The expression of CD94 and NKG2a on CD8 Treg was elevated compared to conventional CD8 T cells. Following in vitro activation, this T cell subset is capable of killing TCR-activated CD4 T cells. These studies identify an endogenous CD8 Treg population in humans and it will now be possible to characterize these cells in a variety of clinical conditions.
pubPublic Library of Science
doi10.1371/journal.pone.0059545
urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3602421/pdf/
pagese59545
volume8
issue3
oafree_for_read