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Host non-inflammatory neutrophils mediate the engraftment of bioengineered vascular networks

Notwithstanding remarkable progress in vascular network engineering, implanted bioengineered microvessels largely fail to form anastomoses with the host vasculature. Here, we demonstrate that implants containing assembled human vascular networks (A-Grafts) fail to engraft due to their inability to e... Full description

Journal Title: Lin R., C. N. Lee, R. Moreno-Luna, J. Neumeyer, B. Piekarski, P. Zhou, M. A. Moses, et al. 2017. “Host non-inflammatory neutrophils mediate the engraftment of bioengineered vascular networks.” Nature biomedical engineering 1 (1): 0081. doi:10.1038/s41551-017-0081. http://dx.doi.org/10.1038/s41551-017-0081.
Main Author: Lin, Ruei-Zeng
Other Authors: Lee, Chin Nien , Moreno-Luna, Rafael , Neumeyer, Joseph , Piekarski, Breanna , Zhou, Pingzhu , Moses, Marsha A. , Sachdev, Monisha , Pu, William T. , Emani, Sitaram , Melero-Martin, Juan M.
Format: Electronic Article Electronic Article
Language: English
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ID: DOI: 10.1038/s41551-017-0081
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title: Host non-inflammatory neutrophils mediate the engraftment of bioengineered vascular networks
format: Article
creator:
  • Lin, Ruei-Zeng
  • Lee, Chin Nien
  • Moreno-Luna, Rafael
  • Neumeyer, Joseph
  • Piekarski, Breanna
  • Zhou, Pingzhu
  • Moses, Marsha A.
  • Sachdev, Monisha
  • Pu, William T.
  • Emani, Sitaram
  • Melero-Martin, Juan M.
subjects:
  • Engineering
ispartof: Lin, R., C. N. Lee, R. Moreno-Luna, J. Neumeyer, B. Piekarski, P. Zhou, M. A. Moses, et al. 2017. “Host non-inflammatory neutrophils mediate the engraftment of bioengineered vascular networks.” Nature biomedical engineering 1 (1): 0081. doi:10.1038/s41551-017-0081. http://dx.doi.org/10.1038/s41551-017-0081.
description: Notwithstanding remarkable progress in vascular network engineering, implanted bioengineered microvessels largely fail to form anastomoses with the host vasculature. Here, we demonstrate that implants containing assembled human vascular networks (A-Grafts) fail to engraft due to their inability to engage non-inflammatory host neutrophils upon implantation into mice. In contrast, unassembled vascular cells (U-Grafts) readily engage alternatively polarized neutrophils, which in turn serve as indispensable mediators of vascular assembly and anastomosis. The depletion of host neutrophils abrogated vascularization in U-Grafts, whereas an adoptive transfer of neutrophils fully restored vascularization in myeloid-depleted mice. Neutrophil engagement was regulated by secreted factors and was progressively silenced as the vasculature matured. Exogenous addition of factors from U-Grafts reengaged neutrophils and enhanced revascularization in A-Grafts, a process that was recapitulated by blocking Notch signaling. Our data suggest that the pro-vascularization potential of neutrophils can be harnessed to improve the engraftment of bioengineered tissues.
language: eng
source:
identifier: DOI: 10.1038/s41551-017-0081
fulltext: fulltext_linktorsrc
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titleHost non-inflammatory neutrophils mediate the engraftment of bioengineered vascular networks
creatorLin, Ruei-Zeng ; Lee, Chin Nien ; Moreno-Luna, Rafael ; Neumeyer, Joseph ; Piekarski, Breanna ; Zhou, Pingzhu ; Moses, Marsha A. ; Sachdev, Monisha ; Pu, William T. ; Emani, Sitaram ; Melero-Martin, Juan M.
ispartofLin, R., C. N. Lee, R. Moreno-Luna, J. Neumeyer, B. Piekarski, P. Zhou, M. A. Moses, et al. 2017. “Host non-inflammatory neutrophils mediate the engraftment of bioengineered vascular networks.” Nature biomedical engineering 1 (1): 0081. doi:10.1038/s41551-017-0081. http://dx.doi.org/10.1038/s41551-017-0081.
identifierDOI: 10.1038/s41551-017-0081
descriptionNotwithstanding remarkable progress in vascular network engineering, implanted bioengineered microvessels largely fail to form anastomoses with the host vasculature. Here, we demonstrate that implants containing assembled human vascular networks (A-Grafts) fail to engraft due to their inability to engage non-inflammatory host neutrophils upon implantation into mice. In contrast, unassembled vascular cells (U-Grafts) readily engage alternatively polarized neutrophils, which in turn serve as indispensable mediators of vascular assembly and anastomosis. The depletion of host neutrophils abrogated vascularization in U-Grafts, whereas an adoptive transfer of neutrophils fully restored vascularization in myeloid-depleted mice. Neutrophil engagement was regulated by secreted factors and was progressively silenced as the vasculature matured. Exogenous addition of factors from U-Grafts reengaged neutrophils and enhanced revascularization in A-Grafts, a process that was recapitulated by blocking Notch signaling. Our data suggest that the pro-vascularization potential of neutrophils can be harnessed to improve the engraftment of bioengineered tissues.
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titleHost non-inflammatory neutrophils mediate the engraftment of bioengineered vascular networks
descriptionNotwithstanding remarkable progress in vascular network engineering, implanted bioengineered microvessels largely fail to form anastomoses with the host vasculature. Here, we demonstrate that implants containing assembled human vascular networks (A-Grafts) fail to engraft due to their inability to engage non-inflammatory host neutrophils upon implantation into mice. In contrast, unassembled vascular cells (U-Grafts) readily engage alternatively polarized neutrophils, which in turn serve as indispensable mediators of vascular assembly and anastomosis. The depletion of host neutrophils abrogated vascularization in U-Grafts, whereas an adoptive transfer of neutrophils fully restored vascularization in myeloid-depleted mice. Neutrophil engagement was regulated by secreted factors and was progressively silenced as the vasculature matured. Exogenous addition of factors from U-Grafts reengaged neutrophils and enhanced revascularization in A-Grafts, a process that was recapitulated by blocking Notch signaling. Our data suggest that the pro-vascularization potential of neutrophils can be harnessed to improve the engraftment of bioengineered tissues.
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abstractNotwithstanding remarkable progress in vascular network engineering, implanted bioengineered microvessels largely fail to form anastomoses with the host vasculature. Here, we demonstrate that implants containing assembled human vascular networks (A-Grafts) fail to engraft due to their inability to engage non-inflammatory host neutrophils upon implantation into mice. In contrast, unassembled vascular cells (U-Grafts) readily engage alternatively polarized neutrophils, which in turn serve as indispensable mediators of vascular assembly and anastomosis. The depletion of host neutrophils abrogated vascularization in U-Grafts, whereas an adoptive transfer of neutrophils fully restored vascularization in myeloid-depleted mice. Neutrophil engagement was regulated by secreted factors and was progressively silenced as the vasculature matured. Exogenous addition of factors from U-Grafts reengaged neutrophils and enhanced revascularization in A-Grafts, a process that was recapitulated by blocking Notch signaling. Our data suggest that the pro-vascularization potential of neutrophils can be harnessed to improve the engraftment of bioengineered tissues.
doi10.1038/s41551-017-0081
urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578427/pdf/
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