schliessen

Filtern

 

Bibliotheken

Obesity alters gene expression for GH/IGF-I axis in mouse mammary fat pads: differential role of cortistatin and somatostatin

Locally produced growth hormone (GH) and IGF-I are key factors in the regulation of mammary gland (MG) development and may be important in breast cancer development/progression. Somatostatin (SST) and cortistatin (CORT) regulate GH/IGF-I axis at various levels, but their role in regulating GH/IGF-I... Full description

Journal Title: PLoS ONE 01 January 2015, Vol.10(3), p.e0120955
Main Author: Alicia Villa-Osaba
Other Authors: Manuel D Gahete , José Córdoba-Chacón , Luis de Lecea , Ana I Pozo-Salas , Francisco Javier Delgado-Lista , Marina Álvarez-Benito , José López-Miranda , Raúl M Luque , Justo P Castaño
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0120955
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: doaj_soai_doaj_org_article_00fa6ed0908d4084aa845e2d03c290fa
title: Obesity alters gene expression for GH/IGF-I axis in mouse mammary fat pads: differential role of cortistatin and somatostatin
format: Article
creator:
  • Alicia Villa-Osaba
  • Manuel D Gahete
  • José Córdoba-Chacón
  • Luis de Lecea
  • Ana I Pozo-Salas
  • Francisco Javier Delgado-Lista
  • Marina Álvarez-Benito
  • José López-Miranda
  • Raúl M Luque
  • Justo P Castaño
subjects:
  • Sciences (General)
ispartof: PLoS ONE, 01 January 2015, Vol.10(3), p.e0120955
description: Locally produced growth hormone (GH) and IGF-I are key factors in the regulation of mammary gland (MG) development and may be important in breast cancer development/progression. Somatostatin (SST) and cortistatin (CORT) regulate GH/IGF-I axis at various levels, but their role in regulating GH/IGF-I in MGs remains unknown. Since obesity alters the expression of these systems in different tissues and is associated to MG (patho) physiology, we sought to investigate the role of SST/CORT in regulating GH/IGF-I system in the MGs of lean and obese mice. Therefore, we analyzed GH/IGF-I as well as SST/CORT and ghrelin systems expression in the mammary fat pads (MFPs) of SST- or CORT-knockout (KO) mice and their respective littermate-controls fed a low-fat (LF) or a high-fat (HF) diet for 16 wks. Our results demonstrate that the majority of the components of GH/IGF-I, SST/CORT and ghrelin systems are locally expressed in mouse MFP. Expression of elements of the GH/IGF-I axis was significantly increased in MFPs of HF-fed control mice while lack of endogenous SST partially suppressed, and lack of CORT completely blunted, the up-regulation observed in obese WT-controls. Since SST/CORT are known to exert an inhibitory role on the GH/IGFI axis, the increase in SST/CORT-receptor sst2 expression in MFPs of HF-fed CORT- and SST-KOs together with an elevation on circulating SST in CORT-KOs could explain the differences observed. These results offer new information on the factors (GH/IGF-I axis) involved in the endocrine/metabolic dysregulation of MFPs in obesity, and suggest that CORT is not a mere SST sibling in regulating MG physiology.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0120955
fulltext: fulltext_linktorsrc
issn:
  • 1932-6203
  • 19326203
url: Link


@attributes
ID1570372235
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordidoai_doaj_org_article_00fa6ed0908d4084aa845e2d03c290fa
sourceiddoaj_s
recordidTN_doaj_soai_doaj_org_article_00fa6ed0908d4084aa845e2d03c290fa
sourcesystemOther
dbidDOA
pqid1667348189
galeid422371090
display
typearticle
titleObesity alters gene expression for GH/IGF-I axis in mouse mammary fat pads: differential role of cortistatin and somatostatin
creatorAlicia Villa-Osaba ; Manuel D Gahete ; José Córdoba-Chacón ; Luis de Lecea ; Ana I Pozo-Salas ; Francisco Javier Delgado-Lista ; Marina Álvarez-Benito ; José López-Miranda ; Raúl M Luque ; Justo P Castaño
ispartofPLoS ONE, 01 January 2015, Vol.10(3), p.e0120955
identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0120955
subjectSciences (General)
descriptionLocally produced growth hormone (GH) and IGF-I are key factors in the regulation of mammary gland (MG) development and may be important in breast cancer development/progression. Somatostatin (SST) and cortistatin (CORT) regulate GH/IGF-I axis at various levels, but their role in regulating GH/IGF-I in MGs remains unknown. Since obesity alters the expression of these systems in different tissues and is associated to MG (patho) physiology, we sought to investigate the role of SST/CORT in regulating GH/IGF-I system in the MGs of lean and obese mice. Therefore, we analyzed GH/IGF-I as well as SST/CORT and ghrelin systems expression in the mammary fat pads (MFPs) of SST- or CORT-knockout (KO) mice and their respective littermate-controls fed a low-fat (LF) or a high-fat (HF) diet for 16 wks. Our results demonstrate that the majority of the components of GH/IGF-I, SST/CORT and ghrelin systems are locally expressed in mouse MFP. Expression of elements of the GH/IGF-I axis was significantly increased in MFPs of HF-fed control mice while lack of endogenous SST partially suppressed, and lack of CORT completely blunted, the up-regulation observed in obese WT-controls. Since SST/CORT are known to exert an inhibitory role on the GH/IGFI axis, the increase in SST/CORT-receptor sst2 expression in MFPs of HF-fed CORT- and SST-KOs together with an elevation on circulating SST in CORT-KOs could explain the differences observed. These results offer new information on the factors (GH/IGF-I axis) involved in the endocrine/metabolic dysregulation of MFPs in obesity, and suggest that CORT is not a mere SST sibling in regulating MG physiology.
languageeng
oafree_for_read
source
version9
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
linktorsrc$$Uhttps://doaj.org/article/00fa6ed0908d4084aa845e2d03c290fa$$EView_full_text_in_DOAJ
search
creatorcontrib
0Alicia Villa-Osaba
1Manuel D Gahete
2José Córdoba-Chacón
3Luis de Lecea
4Ana I Pozo-Salas
5Francisco Javier Delgado-Lista
6Marina Álvarez-Benito
7José López-Miranda
8Raúl M Luque
9Justo P Castaño
titleObesity alters gene expression for GH/IGF-I axis in mouse mammary fat pads: differential role of cortistatin and somatostatin
description

Locally produced growth hormone (GH) and IGF-I are key factors in the regulation of mammary gland (MG) development and may be important in breast cancer development/progression. Somatostatin (SST) and cortistatin (CORT) regulate GH/IGF-I axis at various levels, but their role in regulating GH/IGF-I in MGs remains unknown. Since obesity alters the expression of these systems in different tissues and is associated to MG (patho) physiology, we sought to investigate the role of SST/CORT in regulating GH/IGF-I system in the MGs of lean and obese mice. Therefore, we analyzed GH/IGF-I as well as SST/CORT and ghrelin systems expression in the mammary fat pads (MFPs) of SST- or CORT-knockout (KO) mice and their respective littermate-controls fed a low-fat (LF) or a high-fat (HF) diet for 16 wks. Our results demonstrate that the majority of the components of GH/IGF-I, SST/CORT and ghrelin systems are locally expressed in mouse MFP. Expression of elements of the GH/IGF-I axis was significantly increased in MFPs of HF-fed control mice while lack of endogenous SST partially suppressed, and lack of CORT completely blunted, the up-regulation observed in obese WT-controls. Since SST/CORT are known to exert an inhibitory role on the GH/IGFI axis, the increase in SST/CORT-receptor sst2 expression in MFPs of HF-fed CORT- and SST-KOs together with an elevation on circulating SST in CORT-KOs could explain the differences observed. These results offer new information on the factors (GH/IGF-I axis) involved in the endocrine/metabolic dysregulation of MFPs in obesity, and suggest that CORT is not a mere SST sibling in regulating MG physiology.

subjectSciences (General)
general
0English
1Public Library of Science (PLoS)
210.1371/journal.pone.0120955
3Directory of Open Access Journals (DOAJ)
sourceiddoaj_s
recordiddoaj_soai_doaj_org_article_00fa6ed0908d4084aa845e2d03c290fa
issn
01932-6203
119326203
rsrctypearticle
creationdate2015
addtitlePLoS ONE
searchscope
0doaj_full
1doaj1
scope
0doaj_full
1doaj1
lsr45$$EView_full_text_in_DOAJ
tmp01Directory of Open Access Journals (DOAJ)
tmp02DOA
startdate20150101
enddate20150101
lsr40PLoS ONE, 01 January 2015, Vol.10 (3), p.e0120955
doi10.1371/journal.pone.0120955
citationpf e0120955 vol 10 issue 3
lsr30VSR-Enriched:[pqid, pages, galeid]
sort
titleObesity alters gene expression for GH/IGF-I axis in mouse mammary fat pads: differential role of cortistatin and somatostatin
authorAlicia Villa-Osaba ; Manuel D Gahete ; José Córdoba-Chacón ; Luis de Lecea ; Ana I Pozo-Salas ; Francisco Javier Delgado-Lista ; Marina Álvarez-Benito ; José López-Miranda ; Raúl M Luque ; Justo P Castaño
creationdate20150101
lso0120150101
facets
frbrgroupid1455178422414745539
frbrtype5
newrecords20190714
languageeng
topicSciences (General)
collectionDirectory of Open Access Journals (DOAJ)
prefilterarticles
rsrctypearticles
creatorcontrib
0Alicia Villa-Osaba
1Manuel D Gahete
2José Córdoba-Chacón
3Luis de Lecea
4Ana I Pozo-Salas
5Francisco Javier Delgado-Lista
6Marina Álvarez-Benito
7José López-Miranda
8Raúl M Luque
9Justo P Castaño
jtitlePLoS ONE
creationdate2015
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext_linktorsrc
addata
au
0Alicia Villa-Osaba
1Manuel D Gahete
2José Córdoba-Chacón
3Luis de Lecea
4Ana I Pozo-Salas
5Francisco Javier Delgado-Lista
6Marina Álvarez-Benito
7José López-Miranda
8Raúl M Luque
9Justo P Castaño
atitleObesity alters gene expression for GH/IGF-I axis in mouse mammary fat pads: differential role of cortistatin and somatostatin
jtitlePLoS ONE
risdate20150101
volume10
issue3
spagee0120955
eissn1932-6203
formatjournal
genrearticle
ristypeJOUR
abstract

Locally produced growth hormone (GH) and IGF-I are key factors in the regulation of mammary gland (MG) development and may be important in breast cancer development/progression. Somatostatin (SST) and cortistatin (CORT) regulate GH/IGF-I axis at various levels, but their role in regulating GH/IGF-I in MGs remains unknown. Since obesity alters the expression of these systems in different tissues and is associated to MG (patho) physiology, we sought to investigate the role of SST/CORT in regulating GH/IGF-I system in the MGs of lean and obese mice. Therefore, we analyzed GH/IGF-I as well as SST/CORT and ghrelin systems expression in the mammary fat pads (MFPs) of SST- or CORT-knockout (KO) mice and their respective littermate-controls fed a low-fat (LF) or a high-fat (HF) diet for 16 wks. Our results demonstrate that the majority of the components of GH/IGF-I, SST/CORT and ghrelin systems are locally expressed in mouse MFP. Expression of elements of the GH/IGF-I axis was significantly increased in MFPs of HF-fed control mice while lack of endogenous SST partially suppressed, and lack of CORT completely blunted, the up-regulation observed in obese WT-controls. Since SST/CORT are known to exert an inhibitory role on the GH/IGFI axis, the increase in SST/CORT-receptor sst2 expression in MFPs of HF-fed CORT- and SST-KOs together with an elevation on circulating SST in CORT-KOs could explain the differences observed. These results offer new information on the factors (GH/IGF-I axis) involved in the endocrine/metabolic dysregulation of MFPs in obesity, and suggest that CORT is not a mere SST sibling in regulating MG physiology.

pubPublic Library of Science (PLoS)
doi10.1371/journal.pone.0120955
urlhttps://doaj.org/article/00fa6ed0908d4084aa845e2d03c290fa
lad01PLoS ONE
oafree_for_read
pagese0120955
date2015-01-01