schliessen

Filtern

 

Bibliotheken

Quantum Dots Encapsulated with Canine Parvovirus-Like Particles Improving the Cellular Targeted Labeling

Quantum dots (QDs) have a promising prospect in live-cell imaging and sensing because of unique fluorescence features. QDs aroused significant interest in the bio-imaging field through integrating the fluorescence properties of QDs and the delivery function of biomaterial. The natural tropism of Can... Full description

Journal Title: PLoS ONE 01 January 2015, Vol.10(9), p.e0138883
Main Author: Dan Yan
Other Authors: Bin Wang , Shiqi Sun , Xia Feng , Ye Jin , Xueping Yao , Suizhong Cao , Huichen Guo
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0138883
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: doaj_soai_doaj_org_article_017ca4377c71433f9022d6c7260c4101
title: Quantum Dots Encapsulated with Canine Parvovirus-Like Particles Improving the Cellular Targeted Labeling
format: Article
creator:
  • Dan Yan
  • Bin Wang
  • Shiqi Sun
  • Xia Feng
  • Ye Jin
  • Xueping Yao
  • Suizhong Cao
  • Huichen Guo
subjects:
  • Sciences (General)
ispartof: PLoS ONE, 01 January 2015, Vol.10(9), p.e0138883
description: Quantum dots (QDs) have a promising prospect in live-cell imaging and sensing because of unique fluorescence features. QDs aroused significant interest in the bio-imaging field through integrating the fluorescence properties of QDs and the delivery function of biomaterial. The natural tropism of Canine Parvovirus (CPV) to the transferrin receptor can target specific cells to increase the targeting ability of QDs in cell imaging. CPV virus-like particles (VLPs) from the expression of the CPV-VP2 capsid protein in a prokaryotic expression system were examined to encapsulate the QDs and deliver to cells with an expressed transferrin receptor. CPV-VLPs were used to encapsulate QDs that were modified using 3-mercaptopropionic acid. Gel electrophoresis, fluorescence spectrum, particle size, and transmission electron microscopy verified the conformation of a complex, in which QDs were encapsulated in CPV-VLPs (CPV-VLPs-QDs). When incubated with different cell lines, CPV-VLPs-QDs significantly reduced the cytotoxicity of QDs and selectively labeled the cells with high-level transferrin receptors. Cell-targeted labeling was achieved by utilizing the specific binding between the CPV capsid protein VP2 of VLPs and cellular receptors. CPV-VLPs-QDs, which can mimic the native CPV infection, can recognize and attach to the transferrin receptors on cellular membrane. Therefore, CPV-VLPs can be used as carriers to facilitate the targeted delivery of encapsulated nanomaterials into cells via receptor-mediated pathways. This study confirmed that CPV-VLPs can significantly promote the biocompatibility of nanomaterials and could expand the application of CPV-VLPs in biological medicine.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0138883
fulltext: fulltext_linktorsrc
issn:
  • 1932-6203
  • 19326203
url: Link


@attributes
ID1549209712
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordidoai_doaj_org_article_017ca4377c71433f9022d6c7260c4101
sourceiddoaj_s
recordidTN_doaj_soai_doaj_org_article_017ca4377c71433f9022d6c7260c4101
sourcesystemOther
dbidDOA
pqid1727681754
galeid429601002
display
typearticle
titleQuantum Dots Encapsulated with Canine Parvovirus-Like Particles Improving the Cellular Targeted Labeling
creatorDan Yan ; Bin Wang ; Shiqi Sun ; Xia Feng ; Ye Jin ; Xueping Yao ; Suizhong Cao ; Huichen Guo
ispartofPLoS ONE, 01 January 2015, Vol.10(9), p.e0138883
identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0138883
subjectSciences (General)
descriptionQuantum dots (QDs) have a promising prospect in live-cell imaging and sensing because of unique fluorescence features. QDs aroused significant interest in the bio-imaging field through integrating the fluorescence properties of QDs and the delivery function of biomaterial. The natural tropism of Canine Parvovirus (CPV) to the transferrin receptor can target specific cells to increase the targeting ability of QDs in cell imaging. CPV virus-like particles (VLPs) from the expression of the CPV-VP2 capsid protein in a prokaryotic expression system were examined to encapsulate the QDs and deliver to cells with an expressed transferrin receptor. CPV-VLPs were used to encapsulate QDs that were modified using 3-mercaptopropionic acid. Gel electrophoresis, fluorescence spectrum, particle size, and transmission electron microscopy verified the conformation of a complex, in which QDs were encapsulated in CPV-VLPs (CPV-VLPs-QDs). When incubated with different cell lines, CPV-VLPs-QDs significantly reduced the cytotoxicity of QDs and selectively labeled the cells with high-level transferrin receptors. Cell-targeted labeling was achieved by utilizing the specific binding between the CPV capsid protein VP2 of VLPs and cellular receptors. CPV-VLPs-QDs, which can mimic the native CPV infection, can recognize and attach to the transferrin receptors on cellular membrane. Therefore, CPV-VLPs can be used as carriers to facilitate the targeted delivery of encapsulated nanomaterials into cells via receptor-mediated pathways. This study confirmed that CPV-VLPs can significantly promote the biocompatibility of nanomaterials and could expand the application of CPV-VLPs in biological medicine.
languageeng
oafree_for_read
source
version9
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
linktorsrc$$Uhttps://doaj.org/article/017ca4377c71433f9022d6c7260c4101$$EView_full_text_in_DOAJ
search
creatorcontrib
0Dan Yan
1Bin Wang
2Shiqi Sun
3Xia Feng
4Ye Jin
5Xueping Yao
6Suizhong Cao
7Huichen Guo
titleQuantum Dots Encapsulated with Canine Parvovirus-Like Particles Improving the Cellular Targeted Labeling
description

Quantum dots (QDs) have a promising prospect in live-cell imaging and sensing because of unique fluorescence features. QDs aroused significant interest in the bio-imaging field through integrating the fluorescence properties of QDs and the delivery function of biomaterial. The natural tropism of Canine Parvovirus (CPV) to the transferrin receptor can target specific cells to increase the targeting ability of QDs in cell imaging. CPV virus-like particles (VLPs) from the expression of the CPV-VP2 capsid protein in a prokaryotic expression system were examined to encapsulate the QDs and deliver to cells with an expressed transferrin receptor. CPV-VLPs were used to encapsulate QDs that were modified using 3-mercaptopropionic acid. Gel electrophoresis, fluorescence spectrum, particle size, and transmission electron microscopy verified the conformation of a complex, in which QDs were encapsulated in CPV-VLPs (CPV-VLPs-QDs). When incubated with different cell lines, CPV-VLPs-QDs significantly reduced the cytotoxicity of QDs and selectively labeled the cells with high-level transferrin receptors. Cell-targeted labeling was achieved by utilizing the specific binding between the CPV capsid protein VP2 of VLPs and cellular receptors. CPV-VLPs-QDs, which can mimic the native CPV infection, can recognize and attach to the transferrin receptors on cellular membrane. Therefore, CPV-VLPs can be used as carriers to facilitate the targeted delivery of encapsulated nanomaterials into cells via receptor-mediated pathways. This study confirmed that CPV-VLPs can significantly promote the biocompatibility of nanomaterials and could expand the application of CPV-VLPs in biological medicine.

subjectSciences (General)
general
0English
1Public Library of Science (PLoS)
210.1371/journal.pone.0138883
3Directory of Open Access Journals (DOAJ)
sourceiddoaj_s
recordiddoaj_soai_doaj_org_article_017ca4377c71433f9022d6c7260c4101
issn
01932-6203
119326203
rsrctypearticle
creationdate2015
addtitlePLoS ONE
searchscope
0doaj_full
1doaj1
scope
0doaj_full
1doaj1
lsr45$$EView_full_text_in_DOAJ
tmp01Directory of Open Access Journals (DOAJ)
tmp02DOA
startdate20150101
enddate20150101
lsr40PLoS ONE, 01 January 2015, Vol.10 (9), p.e0138883
doi10.1371/journal.pone.0138883
citationpf e0138883 vol 10 issue 9
lsr30VSR-Enriched:[pqid, pages, galeid]
sort
titleQuantum Dots Encapsulated with Canine Parvovirus-Like Particles Improving the Cellular Targeted Labeling
authorDan Yan ; Bin Wang ; Shiqi Sun ; Xia Feng ; Ye Jin ; Xueping Yao ; Suizhong Cao ; Huichen Guo
creationdate20150101
lso0120150101
facets
frbrgroupid8432129965018686352
frbrtype5
newrecords20190714
languageeng
topicSciences (General)
collectionDirectory of Open Access Journals (DOAJ)
prefilterarticles
rsrctypearticles
creatorcontrib
0Dan Yan
1Bin Wang
2Shiqi Sun
3Xia Feng
4Ye Jin
5Xueping Yao
6Suizhong Cao
7Huichen Guo
jtitlePLoS ONE
creationdate2015
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext_linktorsrc
addata
au
0Dan Yan
1Bin Wang
2Shiqi Sun
3Xia Feng
4Ye Jin
5Xueping Yao
6Suizhong Cao
7Huichen Guo
atitleQuantum Dots Encapsulated with Canine Parvovirus-Like Particles Improving the Cellular Targeted Labeling
jtitlePLoS ONE
risdate20150101
volume10
issue9
spagee0138883
eissn1932-6203
formatjournal
genrearticle
ristypeJOUR
abstract

Quantum dots (QDs) have a promising prospect in live-cell imaging and sensing because of unique fluorescence features. QDs aroused significant interest in the bio-imaging field through integrating the fluorescence properties of QDs and the delivery function of biomaterial. The natural tropism of Canine Parvovirus (CPV) to the transferrin receptor can target specific cells to increase the targeting ability of QDs in cell imaging. CPV virus-like particles (VLPs) from the expression of the CPV-VP2 capsid protein in a prokaryotic expression system were examined to encapsulate the QDs and deliver to cells with an expressed transferrin receptor. CPV-VLPs were used to encapsulate QDs that were modified using 3-mercaptopropionic acid. Gel electrophoresis, fluorescence spectrum, particle size, and transmission electron microscopy verified the conformation of a complex, in which QDs were encapsulated in CPV-VLPs (CPV-VLPs-QDs). When incubated with different cell lines, CPV-VLPs-QDs significantly reduced the cytotoxicity of QDs and selectively labeled the cells with high-level transferrin receptors. Cell-targeted labeling was achieved by utilizing the specific binding between the CPV capsid protein VP2 of VLPs and cellular receptors. CPV-VLPs-QDs, which can mimic the native CPV infection, can recognize and attach to the transferrin receptors on cellular membrane. Therefore, CPV-VLPs can be used as carriers to facilitate the targeted delivery of encapsulated nanomaterials into cells via receptor-mediated pathways. This study confirmed that CPV-VLPs can significantly promote the biocompatibility of nanomaterials and could expand the application of CPV-VLPs in biological medicine.

pubPublic Library of Science (PLoS)
doi10.1371/journal.pone.0138883
urlhttps://doaj.org/article/017ca4377c71433f9022d6c7260c4101
lad01PLoS ONE
oafree_for_read
pagese0138883
date2015-01-01