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Frequent mutations in EGFR, KRAS and TP53 genes in human lung cancer tumors detected by ion torrent DNA sequencing

Lung cancer is the most common malignancy and the leading cause of cancer deaths worldwide. While smoking is by far the leading cause of lung cancer, other environmental and genetic factors influence the development and progression of the cancer. Since unique mutations patterns have been observed in... Full description

Journal Title: PLoS ONE 01 January 2014, Vol.9(4), p.e95228
Main Author: Xin Cai
Other Authors: Jianhui Sheng , Chuanning Tang , Vijayalakshmi Nandakumar , Hua Ye , Hong Ji , Haiying Tang , Yu Qin , Hongwei Guan , Feng Lou , Dandan Zhang , Hong Sun , Haichao Dong , Guangchun Zhang , Zhiyuan Liu , Zhishou Dong , Baishuai Guo , He Yan , Chaowei Yan , Lu Wang , Ziyi Su , Yangyang Li , Lindsey Jones , Xue F Huang , Si-Yi Chen , Taihua Wu , Hongli Lin
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0095228
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recordid: doaj_soai_doaj_org_article_025ba7272ed0458c89a1b441796bffe9
title: Frequent mutations in EGFR, KRAS and TP53 genes in human lung cancer tumors detected by ion torrent DNA sequencing
format: Article
creator:
  • Xin Cai
  • Jianhui Sheng
  • Chuanning Tang
  • Vijayalakshmi Nandakumar
  • Hua Ye
  • Hong Ji
  • Haiying Tang
  • Yu Qin
  • Hongwei Guan
  • Feng Lou
  • Dandan Zhang
  • Hong Sun
  • Haichao Dong
  • Guangchun Zhang
  • Zhiyuan Liu
  • Zhishou Dong
  • Baishuai Guo
  • He Yan
  • Chaowei Yan
  • Lu Wang
  • Ziyi Su
  • Yangyang Li
  • Lindsey Jones
  • Xue F Huang
  • Si-Yi Chen
  • Taihua Wu
  • Hongli Lin
subjects:
  • Sciences (General)
ispartof: PLoS ONE, 01 January 2014, Vol.9(4), p.e95228
description: Lung cancer is the most common malignancy and the leading cause of cancer deaths worldwide. While smoking is by far the leading cause of lung cancer, other environmental and genetic factors influence the development and progression of the cancer. Since unique mutations patterns have been observed in individual cancer samples, identification and characterization of the distinctive lung cancer molecular profile is essential for developing more effective, tailored therapies. Until recently, personalized DNA sequencing to identify genetic mutations in cancer was impractical and expensive. The recent technological advancements in next-generation DNA sequencing, such as the semiconductor-based Ion Torrent sequencing platform, has made DNA sequencing cost and time effective with more reliable results. Using the Ion Torrent Ampliseq Cancer Panel, we sequenced 737 loci from 45 cancer-related genes to identify genetic mutations in 76 human lung cancer samples. The sequencing analysis revealed missense mutations in KRAS, EGFR, and TP53 genes in the breast cancer samples of various histologic types. Thus, this study demonstrates the necessity of sequencing individual human cancers in order to develop personalized drugs or combination therapies to effectively target individual, breast cancer-specific mutations.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0095228
fulltext: fulltext_linktorsrc
issn:
  • 1932-6203
  • 19326203
url: Link


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titleFrequent mutations in EGFR, KRAS and TP53 genes in human lung cancer tumors detected by ion torrent DNA sequencing
creatorXin Cai ; Jianhui Sheng ; Chuanning Tang ; Vijayalakshmi Nandakumar ; Hua Ye ; Hong Ji ; Haiying Tang ; Yu Qin ; Hongwei Guan ; Feng Lou ; Dandan Zhang ; Hong Sun ; Haichao Dong ; Guangchun Zhang ; Zhiyuan Liu ; Zhishou Dong ; Baishuai Guo ; He Yan ; Chaowei Yan ; Lu Wang ; Ziyi Su ; Yangyang Li ; Lindsey Jones ; Xue F Huang ; Si-Yi Chen ; Taihua Wu ; Hongli Lin
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descriptionLung cancer is the most common malignancy and the leading cause of cancer deaths worldwide. While smoking is by far the leading cause of lung cancer, other environmental and genetic factors influence the development and progression of the cancer. Since unique mutations patterns have been observed in individual cancer samples, identification and characterization of the distinctive lung cancer molecular profile is essential for developing more effective, tailored therapies. Until recently, personalized DNA sequencing to identify genetic mutations in cancer was impractical and expensive. The recent technological advancements in next-generation DNA sequencing, such as the semiconductor-based Ion Torrent sequencing platform, has made DNA sequencing cost and time effective with more reliable results. Using the Ion Torrent Ampliseq Cancer Panel, we sequenced 737 loci from 45 cancer-related genes to identify genetic mutations in 76 human lung cancer samples. The sequencing analysis revealed missense mutations in KRAS, EGFR, and TP53 genes in the breast cancer samples of various histologic types. Thus, this study demonstrates the necessity of sequencing individual human cancers in order to develop personalized drugs or combination therapies to effectively target individual, breast cancer-specific mutations.
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Lung cancer is the most common malignancy and the leading cause of cancer deaths worldwide. While smoking is by far the leading cause of lung cancer, other environmental and genetic factors influence the development and progression of the cancer. Since unique mutations patterns have been observed in individual cancer samples, identification and characterization of the distinctive lung cancer molecular profile is essential for developing more effective, tailored therapies. Until recently, personalized DNA sequencing to identify genetic mutations in cancer was impractical and expensive. The recent technological advancements in next-generation DNA sequencing, such as the semiconductor-based Ion Torrent sequencing platform, has made DNA sequencing cost and time effective with more reliable results. Using the Ion Torrent Ampliseq Cancer Panel, we sequenced 737 loci from 45 cancer-related genes to identify genetic mutations in 76 human lung cancer samples. The sequencing analysis revealed missense mutations in KRAS, EGFR, and TP53 genes in the breast cancer samples of various histologic types. Thus, this study demonstrates the necessity of sequencing individual human cancers in order to develop personalized drugs or combination therapies to effectively target individual, breast cancer-specific mutations.

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Lung cancer is the most common malignancy and the leading cause of cancer deaths worldwide. While smoking is by far the leading cause of lung cancer, other environmental and genetic factors influence the development and progression of the cancer. Since unique mutations patterns have been observed in individual cancer samples, identification and characterization of the distinctive lung cancer molecular profile is essential for developing more effective, tailored therapies. Until recently, personalized DNA sequencing to identify genetic mutations in cancer was impractical and expensive. The recent technological advancements in next-generation DNA sequencing, such as the semiconductor-based Ion Torrent sequencing platform, has made DNA sequencing cost and time effective with more reliable results. Using the Ion Torrent Ampliseq Cancer Panel, we sequenced 737 loci from 45 cancer-related genes to identify genetic mutations in 76 human lung cancer samples. The sequencing analysis revealed missense mutations in KRAS, EGFR, and TP53 genes in the breast cancer samples of various histologic types. Thus, this study demonstrates the necessity of sequencing individual human cancers in order to develop personalized drugs or combination therapies to effectively target individual, breast cancer-specific mutations.

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