schliessen

Filtern

 

Bibliotheken

Genetically engineered Lactococcus lactis protect against house dust mite allergy in a BALB/c mouse model

Mucosal vaccine based on lactic acid bacteria is an attractive concept for the prevention and treatment of allergic diseases, but their mechanisms of action in vivo are poorly understood. Therefore, we sought to investigate how recombinant major dust mite allergen Der p2-expressing Lactococcus lacti... Full description

Journal Title: PLoS ONE 01 January 2014, Vol.9(10), p.e109461
Main Author: Chunqing Ai
Other Authors: Qiuxiang Zhang , Chengcheng Ren , Gang Wang , Xiaoming Liu , Fengwei Tian , Jianxin Zhao , Hao Zhang , Yong Q Chen , Wei Chen
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0109461
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: doaj_soai_doaj_org_article_088005e03de1428f8e0e0751ec66822e
title: Genetically engineered Lactococcus lactis protect against house dust mite allergy in a BALB/c mouse model
format: Article
creator:
  • Chunqing Ai
  • Qiuxiang Zhang
  • Chengcheng Ren
  • Gang Wang
  • Xiaoming Liu
  • Fengwei Tian
  • Jianxin Zhao
  • Hao Zhang
  • Yong Q Chen
  • Wei Chen
subjects:
  • Sciences (General)
ispartof: PLoS ONE, 01 January 2014, Vol.9(10), p.e109461
description: Mucosal vaccine based on lactic acid bacteria is an attractive concept for the prevention and treatment of allergic diseases, but their mechanisms of action in vivo are poorly understood. Therefore, we sought to investigate how recombinant major dust mite allergen Der p2-expressing Lactococcus lactis as a mucosal vaccine induced the immune tolerance against house dust mite allergy in a mouse model.Three strains of recombinant L. lactis producing Der p2 in different cell components (extracellular, intracellular and cell wall) were firstly constructed. Their prophylactic potential was evaluated in a Der p2-sensitised mouse model, and immunomodulation properties at the cellular level were determined by measuring cytokine production in vitro.Der p2 expressed in the different recombinant L. lactis strains was recognized by a polyclonal anti-Der p2 antibody. Oral treatment with the recombinant L. lactis prior sensitization significantly prevented the development of airway inflammation in the Der p2-sensitized mice, as determined by the attenuation of inflammatory cells infiltration in the lung tissues and decrease of Th2 cytokines IL-4 and IL-5 levels in bronchoalveolar lavage. In addition, the serum allergen-specific IgE levels were significantly reduced, and the levels of IL-4 in the spleen and mesenteric lymph nodes cell cultures were also markedly decreased upon allergen stimulation in the mice fed with the recombinant L. lactis strains. These protective effects correlated with a significant up-regulation of regulatory T cells in the mesenteric lymph nodes.Oral pretreatment with live recombinant L. lactis prevented the development of allergen-induced airway inflammation primarily by the induction of specific mucosal immune tolerance.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0109461
fulltext: fulltext_linktorsrc
issn:
  • 1932-6203
  • 19326203
url: Link


@attributes
ID1881554428
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordidoai_doaj_org_article_088005e03de1428f8e0e0751ec66822e
sourceiddoaj_s
recordidTN_doaj_soai_doaj_org_article_088005e03de1428f8e0e0751ec66822e
sourcesystemOther
dbidDOA
pqid1609101205
galeid418708137
display
typearticle
titleGenetically engineered Lactococcus lactis protect against house dust mite allergy in a BALB/c mouse model
creatorChunqing Ai ; Qiuxiang Zhang ; Chengcheng Ren ; Gang Wang ; Xiaoming Liu ; Fengwei Tian ; Jianxin Zhao ; Hao Zhang ; Yong Q Chen ; Wei Chen
ispartofPLoS ONE, 01 January 2014, Vol.9(10), p.e109461
identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0109461
subjectSciences (General)
descriptionMucosal vaccine based on lactic acid bacteria is an attractive concept for the prevention and treatment of allergic diseases, but their mechanisms of action in vivo are poorly understood. Therefore, we sought to investigate how recombinant major dust mite allergen Der p2-expressing Lactococcus lactis as a mucosal vaccine induced the immune tolerance against house dust mite allergy in a mouse model.Three strains of recombinant L. lactis producing Der p2 in different cell components (extracellular, intracellular and cell wall) were firstly constructed. Their prophylactic potential was evaluated in a Der p2-sensitised mouse model, and immunomodulation properties at the cellular level were determined by measuring cytokine production in vitro.Der p2 expressed in the different recombinant L. lactis strains was recognized by a polyclonal anti-Der p2 antibody. Oral treatment with the recombinant L. lactis prior sensitization significantly prevented the development of airway inflammation in the Der p2-sensitized mice, as determined by the attenuation of inflammatory cells infiltration in the lung tissues and decrease of Th2 cytokines IL-4 and IL-5 levels in bronchoalveolar lavage. In addition, the serum allergen-specific IgE levels were significantly reduced, and the levels of IL-4 in the spleen and mesenteric lymph nodes cell cultures were also markedly decreased upon allergen stimulation in the mice fed with the recombinant L. lactis strains. These protective effects correlated with a significant up-regulation of regulatory T cells in the mesenteric lymph nodes.Oral pretreatment with live recombinant L. lactis prevented the development of allergen-induced airway inflammation primarily by the induction of specific mucosal immune tolerance.
languageeng
oafree_for_read
source
version7
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
linktorsrc$$Uhttps://doaj.org/article/088005e03de1428f8e0e0751ec66822e$$EView_full_text_in_DOAJ
search
creatorcontrib
0Chunqing Ai
1Qiuxiang Zhang
2Chengcheng Ren
3Gang Wang
4Xiaoming Liu
5Fengwei Tian
6Jianxin Zhao
7Hao Zhang
8Yong Q Chen
9Wei Chen
titleGenetically engineered Lactococcus lactis protect against house dust mite allergy in a BALB/c mouse model
description

Mucosal vaccine based on lactic acid bacteria is an attractive concept for the prevention and treatment of allergic diseases, but their mechanisms of action in vivo are poorly understood. Therefore, we sought to investigate how recombinant major dust mite allergen Der p2-expressing Lactococcus lactis as a mucosal vaccine induced the immune tolerance against house dust mite allergy in a mouse model.Three strains of recombinant L. lactis producing Der p2 in different cell components (extracellular, intracellular and cell wall) were firstly constructed. Their prophylactic potential was evaluated in a Der p2-sensitised mouse model, and immunomodulation properties at the cellular level were determined by measuring cytokine production in vitro.Der p2 expressed in the different recombinant L. lactis strains was recognized by a polyclonal anti-Der p2 antibody. Oral treatment with the recombinant L. lactis prior sensitization significantly prevented the development of airway inflammation in the Der p2-sensitized mice, as determined by the attenuation of inflammatory cells infiltration in the lung tissues and decrease of Th2 cytokines IL-4 and IL-5 levels in bronchoalveolar lavage. In addition, the serum allergen-specific IgE levels were significantly reduced, and the levels of IL-4 in the spleen and mesenteric lymph nodes cell cultures were also markedly decreased upon allergen stimulation in the mice fed with the recombinant L. lactis strains. These protective effects correlated with a significant up-regulation of regulatory T cells in the mesenteric lymph nodes.Oral pretreatment with live recombinant L. lactis prevented the development of allergen-induced airway inflammation primarily by the induction of specific mucosal immune tolerance.

subjectSciences (General)
general
0English
1Public Library of Science (PLoS)
210.1371/journal.pone.0109461
3Directory of Open Access Journals (DOAJ)
sourceiddoaj_s
recordiddoaj_soai_doaj_org_article_088005e03de1428f8e0e0751ec66822e
issn
01932-6203
119326203
rsrctypearticle
creationdate2014
addtitlePLoS ONE
searchscope
0doaj_full
1doaj1
scope
0doaj_full
1doaj1
lsr45$$EView_full_text_in_DOAJ
tmp01Directory of Open Access Journals (DOAJ)
tmp02DOA
startdate20140101
enddate20140101
lsr40PLoS ONE, 01 January 2014, Vol.9 (10), p.e109461
doi10.1371/journal.pone.0109461
citationpf e109461 vol 9 issue 10
lsr30VSR-Enriched:[pqid, pages, galeid]
sort
titleGenetically engineered Lactococcus lactis protect against house dust mite allergy in a BALB/c mouse model
authorChunqing Ai ; Qiuxiang Zhang ; Chengcheng Ren ; Gang Wang ; Xiaoming Liu ; Fengwei Tian ; Jianxin Zhao ; Hao Zhang ; Yong Q Chen ; Wei Chen
creationdate20140101
lso0120140101
facets
frbrgroupid6285643900098769102
frbrtype5
newrecords20190714
languageeng
topicSciences (General)
collectionDirectory of Open Access Journals (DOAJ)
prefilterarticles
rsrctypearticles
creatorcontrib
0Chunqing Ai
1Qiuxiang Zhang
2Chengcheng Ren
3Gang Wang
4Xiaoming Liu
5Fengwei Tian
6Jianxin Zhao
7Hao Zhang
8Yong Q Chen
9Wei Chen
jtitlePLoS ONE
creationdate2014
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext_linktorsrc
addata
au
0Chunqing Ai
1Qiuxiang Zhang
2Chengcheng Ren
3Gang Wang
4Xiaoming Liu
5Fengwei Tian
6Jianxin Zhao
7Hao Zhang
8Yong Q Chen
9Wei Chen
atitleGenetically engineered Lactococcus lactis protect against house dust mite allergy in a BALB/c mouse model
jtitlePLoS ONE
risdate20140101
volume9
issue10
spagee109461
eissn1932-6203
formatjournal
genrearticle
ristypeJOUR
abstract

Mucosal vaccine based on lactic acid bacteria is an attractive concept for the prevention and treatment of allergic diseases, but their mechanisms of action in vivo are poorly understood. Therefore, we sought to investigate how recombinant major dust mite allergen Der p2-expressing Lactococcus lactis as a mucosal vaccine induced the immune tolerance against house dust mite allergy in a mouse model.Three strains of recombinant L. lactis producing Der p2 in different cell components (extracellular, intracellular and cell wall) were firstly constructed. Their prophylactic potential was evaluated in a Der p2-sensitised mouse model, and immunomodulation properties at the cellular level were determined by measuring cytokine production in vitro.Der p2 expressed in the different recombinant L. lactis strains was recognized by a polyclonal anti-Der p2 antibody. Oral treatment with the recombinant L. lactis prior sensitization significantly prevented the development of airway inflammation in the Der p2-sensitized mice, as determined by the attenuation of inflammatory cells infiltration in the lung tissues and decrease of Th2 cytokines IL-4 and IL-5 levels in bronchoalveolar lavage. In addition, the serum allergen-specific IgE levels were significantly reduced, and the levels of IL-4 in the spleen and mesenteric lymph nodes cell cultures were also markedly decreased upon allergen stimulation in the mice fed with the recombinant L. lactis strains. These protective effects correlated with a significant up-regulation of regulatory T cells in the mesenteric lymph nodes.Oral pretreatment with live recombinant L. lactis prevented the development of allergen-induced airway inflammation primarily by the induction of specific mucosal immune tolerance.

pubPublic Library of Science (PLoS)
doi10.1371/journal.pone.0109461
urlhttps://doaj.org/article/088005e03de1428f8e0e0751ec66822e
lad01PLoS ONE
oafree_for_read
pagese109461
date2014-01-01