schliessen

Filtern

 

Bibliotheken

An Apta-Biosensor for Colon Cancer Diagnostics

This paper reports the design and implementation of an aptasensor using a modified KCHA10a aptamer. This aptasensor consists of a functionalized electrodes using various materials including 11-mercaptoandecanoic acid (11-MUA) and modified KCHA10a aptamer. The HCT 116, HT 29 and HEp-2 cell lines are... Full description

Journal Title: Sensors 01 September 2015, Vol.15(9), pp.22291-22303
Main Author: Mojgan Ahmadzadeh Raji
Other Authors: Ghasem Amoabediny , Parviz Tajik , Morteza Hosseini , Ebrahim Ghafar-Zadeh
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1424-8220 ; DOI: 10.3390/s150922291
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: doaj_soai_doaj_org_article_0d0c79505e014ea5b1f53af68c0a3785
title: An Apta-Biosensor for Colon Cancer Diagnostics
format: Article
creator:
  • Mojgan Ahmadzadeh Raji
  • Ghasem Amoabediny
  • Parviz Tajik
  • Morteza Hosseini
  • Ebrahim Ghafar-Zadeh
subjects:
  • Aptasensor
  • Aptamer
  • Cyclic Voltammetry (Cv)
  • Colon Cancer
  • Biosensor
  • Fluorescence Labeling
  • Engineering
ispartof: Sensors, 01 September 2015, Vol.15(9), pp.22291-22303
description: This paper reports the design and implementation of an aptasensor using a modified KCHA10a aptamer. This aptasensor consists of a functionalized electrodes using various materials including 11-mercaptoandecanoic acid (11-MUA) and modified KCHA10a aptamer. The HCT 116, HT 29 and HEp-2 cell lines are used in this study to demonstrate the functionality of aptasensor for colon cancer detection purposes. Flow cytometry, fluorescence microscopy and electrochemical cyclic voltammetry are used to verify the binding between the target cells and aptamer. The limit of detection (LOD) of this aptasensor is equal to seven cancer cells. Based on the experimental results, the proposed sensor can be employed for point-of-care cancer disease diagnostics.
language: eng
source:
identifier: E-ISSN: 1424-8220 ; DOI: 10.3390/s150922291
fulltext: fulltext_linktorsrc
issn:
  • 1424-8220
  • 14248220
url: Link


@attributes
ID973173209
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordidoai_doaj_org_article_0d0c79505e014ea5b1f53af68c0a3785
sourceiddoaj_s
recordidTN_doaj_soai_doaj_org_article_0d0c79505e014ea5b1f53af68c0a3785
sourcesystemPC
dbidDOA
pqid1753462687
display
typearticle
titleAn Apta-Biosensor for Colon Cancer Diagnostics
creatorMojgan Ahmadzadeh Raji ; Ghasem Amoabediny ; Parviz Tajik ; Morteza Hosseini ; Ebrahim Ghafar-Zadeh
ispartofSensors, 01 September 2015, Vol.15(9), pp.22291-22303
identifierE-ISSN: 1424-8220 ; DOI: 10.3390/s150922291
subjectAptasensor ; Aptamer ; Cyclic Voltammetry (Cv) ; Colon Cancer ; Biosensor ; Fluorescence Labeling ; Engineering
languageeng
oafree_for_read
source
descriptionThis paper reports the design and implementation of an aptasensor using a modified KCHA10a aptamer. This aptasensor consists of a functionalized electrodes using various materials including 11-mercaptoandecanoic acid (11-MUA) and modified KCHA10a aptamer. The HCT 116, HT 29 and HEp-2 cell lines are used in this study to demonstrate the functionality of aptasensor for colon cancer detection purposes. Flow cytometry, fluorescence microscopy and electrochemical cyclic voltammetry are used to verify the binding between the target cells and aptamer. The limit of detection (LOD) of this aptasensor is equal to seven cancer cells. Based on the experimental results, the proposed sensor can be employed for point-of-care cancer disease diagnostics.
version7
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
linktorsrc$$Uhttps://doaj.org/article/0d0c79505e014ea5b1f53af68c0a3785$$EView_full_text_in_DOAJ
search
creatorcontrib
0Mojgan Ahmadzadeh Raji
1Ghasem Amoabediny
2Parviz Tajik
3Morteza Hosseini
4Ebrahim Ghafar-Zadeh
titleAn Apta-Biosensor for Colon Cancer Diagnostics
description

This paper reports the design and implementation of an aptasensor using a modified KCHA10a aptamer. This aptasensor consists of a functionalized electrodes using various materials including 11-mercaptoandecanoic acid (11-MUA) and modified KCHA10a aptamer. The HCT 116, HT 29 and HEp-2 cell lines are used in this study to demonstrate the functionality of aptasensor for colon cancer detection purposes. Flow cytometry, fluorescence microscopy and electrochemical cyclic voltammetry are used to verify the binding between the target cells and aptamer. The limit of detection (LOD) of this aptasensor is equal to seven cancer cells. Based on the experimental results, the proposed sensor can be employed for point-of-care cancer disease diagnostics.

subject
0Aptasensor
1Aptamer
2Cyclic Voltammetry (Cv)
3Colon Cancer
4Biosensor
5Fluorescence Labeling
6Engineering
general
0English
1MDPI AG
210.3390/s150922291
3Directory of Open Access Journals (DOAJ)
sourceiddoaj_s
recordiddoaj_soai_doaj_org_article_0d0c79505e014ea5b1f53af68c0a3785
issn
01424-8220
114248220
rsrctypearticle
creationdate2015
addtitleSensors
searchscope
0doaj_full
1doaj1
scope
0doaj_full
1doaj1
lsr45$$EView_full_text_in_DOAJ
tmp01Directory of Open Access Journals (DOAJ)
tmp02DOA
startdate20150901
enddate20150901
lsr40Sensors, 01 September 2015, Vol.15 (9), pp.22291-22303
doi10.3390/s150922291
citationpf 22291 pt 22303 vol 15 issue 9
lsr30VSR-Enriched:[pqid, description]
sort
titleAn Apta-Biosensor for Colon Cancer Diagnostics
authorMojgan Ahmadzadeh Raji ; Ghasem Amoabediny ; Parviz Tajik ; Morteza Hosseini ; Ebrahim Ghafar-Zadeh
creationdate20150901
lso0120150901
facets
frbrgroupid8687019889582637723
frbrtype5
newrecords20200226
languageeng
topic
0Aptasensor
1Aptamer
2Cyclic Voltammetry (Cv)
3Colon Cancer
4Biosensor
5Fluorescence Labeling
6Engineering
collectionDirectory of Open Access Journals (DOAJ)
prefilterarticles
rsrctypearticles
creatorcontrib
0Mojgan Ahmadzadeh Raji
1Ghasem Amoabediny
2Parviz Tajik
3Morteza Hosseini
4Ebrahim Ghafar-Zadeh
jtitleSensors
creationdate2015
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext_linktorsrc
addata
au
0Mojgan Ahmadzadeh Raji
1Ghasem Amoabediny
2Parviz Tajik
3Morteza Hosseini
4Ebrahim Ghafar-Zadeh
atitleAn Apta-Biosensor for Colon Cancer Diagnostics
jtitleSensors
risdate20150901
volume15
issue9
spage22291
epage22303
pages22291-22303
eissn1424-8220
formatjournal
genrearticle
ristypeJOUR
abstract

This paper reports the design and implementation of an aptasensor using a modified KCHA10a aptamer. This aptasensor consists of a functionalized electrodes using various materials including 11-mercaptoandecanoic acid (11-MUA) and modified KCHA10a aptamer. The HCT 116, HT 29 and HEp-2 cell lines are used in this study to demonstrate the functionality of aptasensor for colon cancer detection purposes. Flow cytometry, fluorescence microscopy and electrochemical cyclic voltammetry are used to verify the binding between the target cells and aptamer. The limit of detection (LOD) of this aptasensor is equal to seven cancer cells. Based on the experimental results, the proposed sensor can be employed for point-of-care cancer disease diagnostics.

pubMDPI AG
doi10.3390/s150922291
urlhttps://doaj.org/article/0d0c79505e014ea5b1f53af68c0a3785
lad01Sensors
oafree_for_read
date2015-09-01