schliessen

Filtern

 

Bibliotheken

Mutation of a Cuticle Protein Gene, BmCPG10, Is Responsible for Silkworm Non-Moulting in the 2nd Instar Mutant

In the silkworm, metamorphosis and moulting are regulated by ecdysone hormone and juvenile hormone. The subject in the present study is a silkworm mutant that does not moult in the 2.sup.nd instar (nm2). Genetic analysis indicated that the nm2 mutation is controlled by a recessive gene and is homozy... Full description

Journal Title: PLoS ONE 01 January 2016, Vol.11(4), p.e0153549
Main Author: Fan Wu
Other Authors: Pingyang Wang , Qiaoling Zhao , Lequn Kang , Dingguo Xia , Zhiyong Qiu , Shunming Tang , Muwang Li , Xingjia Shen , Guozheng Zhang
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0153549
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: doaj_soai_doaj_org_article_20b003444c4148db829531eb84271ab2
title: Mutation of a Cuticle Protein Gene, BmCPG10, Is Responsible for Silkworm Non-Moulting in the 2nd Instar Mutant
format: Article
creator:
  • Fan Wu
  • Pingyang Wang
  • Qiaoling Zhao
  • Lequn Kang
  • Dingguo Xia
  • Zhiyong Qiu
  • Shunming Tang
  • Muwang Li
  • Xingjia Shen
  • Guozheng Zhang
subjects:
  • Sciences (General)
ispartof: PLoS ONE, 01 January 2016, Vol.11(4), p.e0153549
description: In the silkworm, metamorphosis and moulting are regulated by ecdysone hormone and juvenile hormone. The subject in the present study is a silkworm mutant that does not moult in the 2.sup.nd instar (nm2). Genetic analysis indicated that the nm2 mutation is controlled by a recessive gene and is homozygous lethal. Based on positional cloning, nm2 was located in a region approximately 275 kb on the 5.sup.th linkage group by eleven SSR polymorphism markers. In this specific range, according to the transcriptional expression of thirteen genes and cloning, the relative expression level of the BmCPG10 gene that encodes a cuticle protein was lower than the expression level of the wild-type gene. Moreover, this gene's structure differs from that of the wild-type gene: there is a deletion of 217 bp in its open reading frame, which resulted in a change in the protein it encoded. The BmCPG10 mRNA was detectable throughout silkworm development from the egg to the moth. This mRNA was low in the pre-moulting and moulting stages of each instar but was high in the gluttonous stage and in newly exuviated larvae. The BmCPG10 mRNA showed high expression levels in the epidermis, head and trachea, while the expression levels were low in the midgut, Malpighian tubule, prothoracic gland, haemolymph and ventral nerve cord. The ecdysone titre was determined by ELISA, and the results demonstrated that the ecdysone titre of nm2 larvae was lower than that of the wild-type larvae. The nm2 mutant could be rescued by feeding 20-hydroxyecdysone, cholesterol and 7-dehydrocholesterol (7dC), but the rescued nm2 only developed to the 4.sup.th instar and subsequently died. The moulting time of silkworms could be delayed by BmCPG10 RNAi. Thus, we speculated that the mutation of BmCPG10 was responsible for the silkworm mutant that did not moult in the 2.sup.nd instar.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0153549
fulltext: fulltext_linktorsrc
issn:
  • 1932-6203
  • 19326203
url: Link


@attributes
ID1020869181
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordidoai_doaj_org_article_20b003444c4148db829531eb84271ab2
sourceiddoaj_s
recordidTN_doaj_soai_doaj_org_article_20b003444c4148db829531eb84271ab2
sourcesystemPC
dbidDOA
pqid1805499688
galeid453426930
display
typearticle
titleMutation of a Cuticle Protein Gene, BmCPG10, Is Responsible for Silkworm Non-Moulting in the 2nd Instar Mutant
creatorFan Wu ; Pingyang Wang ; Qiaoling Zhao ; Lequn Kang ; Dingguo Xia ; Zhiyong Qiu ; Shunming Tang ; Muwang Li ; Xingjia Shen ; Guozheng Zhang
ispartofPLoS ONE, 01 January 2016, Vol.11(4), p.e0153549
identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0153549
subjectSciences (General)
languageeng
oafree_for_read
source
descriptionIn the silkworm, metamorphosis and moulting are regulated by ecdysone hormone and juvenile hormone. The subject in the present study is a silkworm mutant that does not moult in the 2.sup.nd instar (nm2). Genetic analysis indicated that the nm2 mutation is controlled by a recessive gene and is homozygous lethal. Based on positional cloning, nm2 was located in a region approximately 275 kb on the 5.sup.th linkage group by eleven SSR polymorphism markers. In this specific range, according to the transcriptional expression of thirteen genes and cloning, the relative expression level of the BmCPG10 gene that encodes a cuticle protein was lower than the expression level of the wild-type gene. Moreover, this gene's structure differs from that of the wild-type gene: there is a deletion of 217 bp in its open reading frame, which resulted in a change in the protein it encoded. The BmCPG10 mRNA was detectable throughout silkworm development from the egg to the moth. This mRNA was low in the pre-moulting and moulting stages of each instar but was high in the gluttonous stage and in newly exuviated larvae. The BmCPG10 mRNA showed high expression levels in the epidermis, head and trachea, while the expression levels were low in the midgut, Malpighian tubule, prothoracic gland, haemolymph and ventral nerve cord. The ecdysone titre was determined by ELISA, and the results demonstrated that the ecdysone titre of nm2 larvae was lower than that of the wild-type larvae. The nm2 mutant could be rescued by feeding 20-hydroxyecdysone, cholesterol and 7-dehydrocholesterol (7dC), but the rescued nm2 only developed to the 4.sup.th instar and subsequently died. The moulting time of silkworms could be delayed by BmCPG10 RNAi. Thus, we speculated that the mutation of BmCPG10 was responsible for the silkworm mutant that did not moult in the 2.sup.nd instar.
version9
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
linktorsrc$$Uhttps://doaj.org/article/20b003444c4148db829531eb84271ab2$$EView_full_text_in_DOAJ
search
creatorcontrib
0Fan Wu
1Pingyang Wang
2Qiaoling Zhao
3Lequn Kang
4Dingguo Xia
5Zhiyong Qiu
6Shunming Tang
7Muwang Li
8Xingjia Shen
9Guozheng Zhang
titleMutation of a Cuticle Protein Gene, BmCPG10, Is Responsible for Silkworm Non-Moulting in the 2nd Instar Mutant
description

In the silkworm, metamorphosis and moulting are regulated by ecdysone hormone and juvenile hormone. The subject in the present study is a silkworm mutant that does not moult in the 2nd instar (nm2). Genetic analysis indicated that the nm2 mutation is controlled by a recessive gene and is homozygous lethal. Based on positional cloning, nm2 was located in a region approximately 275 kb on the 5th linkage group by eleven SSR polymorphism markers. In this specific range, according to the transcriptional expression of thirteen genes and cloning, the relative expression level of the BmCPG10 gene that encodes a cuticle protein was lower than the expression level of the wild-type gene. Moreover, this gene's structure differs from that of the wild-type gene: there is a deletion of 217 bp in its open reading frame, which resulted in a change in the protein it encoded. The BmCPG10 mRNA was detectable throughout silkworm development from the egg to the moth. This mRNA was low in the pre-moulting...

subjectSciences (General)
general
0English
1Public Library of Science (PLoS)
210.1371/journal.pone.0153549
3Directory of Open Access Journals (DOAJ)
sourceiddoaj_s
recordiddoaj_soai_doaj_org_article_20b003444c4148db829531eb84271ab2
issn
01932-6203
119326203
rsrctypearticle
creationdate2016
addtitlePLoS ONE
searchscope
0doaj_full
1doaj1
scope
0doaj_full
1doaj1
lsr45$$EView_full_text_in_DOAJ
tmp01Directory of Open Access Journals (DOAJ)
tmp02DOA
startdate20160101
enddate20160101
lsr40PLoS ONE, 01 January 2016, Vol.11 (4), p.e0153549
doi10.1371/journal.pone.0153549
citationpf e0153549 vol 11 issue 4
lsr30VSR-Enriched:[pqid, pages, galeid, description]
sort
titleMutation of a Cuticle Protein Gene, BmCPG10, Is Responsible for Silkworm Non-Moulting in the 2nd Instar Mutant
authorFan Wu ; Pingyang Wang ; Qiaoling Zhao ; Lequn Kang ; Dingguo Xia ; Zhiyong Qiu ; Shunming Tang ; Muwang Li ; Xingjia Shen ; Guozheng Zhang
creationdate20160101
lso0120160101
facets
frbrgroupid8351562521086836818
frbrtype5
newrecords20200226
languageeng
topicSciences (General)
collectionDirectory of Open Access Journals (DOAJ)
prefilterarticles
rsrctypearticles
creatorcontrib
0Fan Wu
1Pingyang Wang
2Qiaoling Zhao
3Lequn Kang
4Dingguo Xia
5Zhiyong Qiu
6Shunming Tang
7Muwang Li
8Xingjia Shen
9Guozheng Zhang
jtitlePLoS ONE
creationdate2016
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext_linktorsrc
addata
au
0Fan Wu
1Pingyang Wang
2Qiaoling Zhao
3Lequn Kang
4Dingguo Xia
5Zhiyong Qiu
6Shunming Tang
7Muwang Li
8Xingjia Shen
9Guozheng Zhang
atitleMutation of a Cuticle Protein Gene, BmCPG10, Is Responsible for Silkworm Non-Moulting in the 2nd Instar Mutant
jtitlePLoS ONE
risdate20160101
volume11
issue4
spagee0153549
eissn1932-6203
formatjournal
genrearticle
ristypeJOUR
abstract

In the silkworm, metamorphosis and moulting are regulated by ecdysone hormone and juvenile hormone. The subject in the present study is a silkworm mutant that does not moult in the 2nd instar (nm2). Genetic analysis indicated that the nm2 mutation is controlled by a recessive gene and is homozygous lethal. Based on positional cloning, nm2 was located in a region approximately 275 kb on the 5th linkage group by eleven SSR polymorphism markers. In this specific range, according to the transcriptional expression of thirteen genes and cloning, the relative expression level of the BmCPG10 gene that encodes a cuticle protein was lower than the expression level of the wild-type gene. Moreover, this gene's structure differs from that of the wild-type gene: there is a deletion of 217 bp in its open reading frame, which resulted in a change in the protein it encoded. The BmCPG10 mRNA was detectable throughout silkworm development from the egg to the moth. This mRNA was low in the pre-moulting...

pubPublic Library of Science (PLoS)
doi10.1371/journal.pone.0153549
urlhttps://doaj.org/article/20b003444c4148db829531eb84271ab2
lad01PLoS ONE
oafree_for_read
pagese0153549
date2016-01-01