schliessen

Filtern

 

Bibliotheken

Comparison of Fecal Microbiota in Children with Autism Spectrum Disorders and Neurotypical Siblings in the Simons Simplex Collection

In order to assess potential associations between autism spectrum disorder (ASD) phenotype, functional GI disorders and fecal microbiota, we recruited simplex families, which had only a single ASD proband and neurotypical (NT) siblings, through the Simons Simplex Community at the Interactive Autism... Full description

Journal Title: PLoS ONE 01 January 2015, Vol.10(10), p.e0137725
Main Author: Joshua S Son
Other Authors: Ling J Zheng , Leahana M Rowehl , Xinyu Tian , Yuanhao Zhang , Wei Zhu , Leighann Litcher-Kelly , Kenneth D Gadow , Grace Gathungu , Charles E Robertson , Diana Ir , Daniel N Frank , Ellen Li
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0137725
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: doaj_soai_doaj_org_article_76c2ad09a4bd41098a98237d17604e9b
title: Comparison of Fecal Microbiota in Children with Autism Spectrum Disorders and Neurotypical Siblings in the Simons Simplex Collection
format: Article
creator:
  • Joshua S Son
  • Ling J Zheng
  • Leahana M Rowehl
  • Xinyu Tian
  • Yuanhao Zhang
  • Wei Zhu
  • Leighann Litcher-Kelly
  • Kenneth D Gadow
  • Grace Gathungu
  • Charles E Robertson
  • Diana Ir
  • Daniel N Frank
  • Ellen Li
subjects:
  • Sciences (General)
ispartof: PLoS ONE, 01 January 2015, Vol.10(10), p.e0137725
description: In order to assess potential associations between autism spectrum disorder (ASD) phenotype, functional GI disorders and fecal microbiota, we recruited simplex families, which had only a single ASD proband and neurotypical (NT) siblings, through the Simons Simplex Community at the Interactive Autism Network (SSC@IAN). Fecal samples and metadata related to functional GI disorders and diet were collected from ASD probands and NT siblings of ASD probands (age 7-14). Functional gastrointestinal disorders (FGID) were assessed using the parent-completed ROME III questionnaire for pediatric FGIDs, and problem behaviors were assessed using the Child Behavior Check List (CBCL). Targeted quantitative polymerase chain reaction (qPCR) assays were conducted on selected taxa implicated in ASD, including Sutterella spp., Bacteroidetes spp. and Prevotella spp. Illumina sequencing of the V1V2 and the V1V3 regions of the bacterial 16S rRNA genes from fecal DNA was performed to an average depth of 208,000 and 107,000 high-quality reads respectively. Twenty-five of 59 ASD children and 13 of 44 NT siblings met ROME III criteria for at least one FGID. Functional constipation was more prevalent in ASD (17 of 59) compared to NT siblings (6 of 44, P = 0.035). The mean CBCL scores in NT siblings with FGID, ASD children with FGID and ASD without FGID were comparably higher (58-62 vs. 44, P < 0.0001) when compared to NT children without FGID. There was no significant difference in macronutrient intake between ASD and NT siblings. There was no significant difference in ASD severity scores between ASD children with and without FGID. No significant difference in diversity or overall microbial composition was detected between ASD children with NT siblings. Exploratory analysis of the 16S rRNA sequencing data, however, identified several low abundance taxa binned at the genus level that were associated with ASD and/or first order ASD*FGID interactions (FDR
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0137725
fulltext: fulltext_linktorsrc
issn:
  • 1932-6203
  • 19326203
url: Link


@attributes
ID718692130
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordidoai_doaj_org_article_76c2ad09a4bd41098a98237d17604e9b
sourceiddoaj_s
recordidTN_doaj_soai_doaj_org_article_76c2ad09a4bd41098a98237d17604e9b
sourcesystemOther
dbidDOA
pqid1823943281
galeid430457389
display
typearticle
titleComparison of Fecal Microbiota in Children with Autism Spectrum Disorders and Neurotypical Siblings in the Simons Simplex Collection
creatorJoshua S Son ; Ling J Zheng ; Leahana M Rowehl ; Xinyu Tian ; Yuanhao Zhang ; Wei Zhu ; Leighann Litcher-Kelly ; Kenneth D Gadow ; Grace Gathungu ; Charles E Robertson ; Diana Ir ; Daniel N Frank ; Ellen Li
ispartofPLoS ONE, 01 January 2015, Vol.10(10), p.e0137725
identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0137725
subjectSciences (General)
descriptionIn order to assess potential associations between autism spectrum disorder (ASD) phenotype, functional GI disorders and fecal microbiota, we recruited simplex families, which had only a single ASD proband and neurotypical (NT) siblings, through the Simons Simplex Community at the Interactive Autism Network (SSC@IAN). Fecal samples and metadata related to functional GI disorders and diet were collected from ASD probands and NT siblings of ASD probands (age 7-14). Functional gastrointestinal disorders (FGID) were assessed using the parent-completed ROME III questionnaire for pediatric FGIDs, and problem behaviors were assessed using the Child Behavior Check List (CBCL). Targeted quantitative polymerase chain reaction (qPCR) assays were conducted on selected taxa implicated in ASD, including Sutterella spp., Bacteroidetes spp. and Prevotella spp. Illumina sequencing of the V1V2 and the V1V3 regions of the bacterial 16S rRNA genes from fecal DNA was performed to an average depth of 208,000 and 107,000 high-quality reads respectively. Twenty-five of 59 ASD children and 13 of 44 NT siblings met ROME III criteria for at least one FGID. Functional constipation was more prevalent in ASD (17 of 59) compared to NT siblings (6 of 44, P = 0.035). The mean CBCL scores in NT siblings with FGID, ASD children with FGID and ASD without FGID were comparably higher (58-62 vs. 44, P < 0.0001) when compared to NT children without FGID. There was no significant difference in macronutrient intake between ASD and NT siblings. There was no significant difference in ASD severity scores between ASD children with and without FGID. No significant difference in diversity or overall microbial composition was detected between ASD children with NT siblings. Exploratory analysis of the 16S rRNA sequencing data, however, identified several low abundance taxa binned at the genus level that were associated with ASD and/or first order ASD*FGID interactions (FDR <0.1).
languageeng
oafree_for_read
source
version9
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
linktorsrc$$Uhttps://doaj.org/article/76c2ad09a4bd41098a98237d17604e9b$$EView_full_text_in_DOAJ
search
creatorcontrib
0Joshua S Son
1Ling J Zheng
2Leahana M Rowehl
3Xinyu Tian
4Yuanhao Zhang
5Wei Zhu
6Leighann Litcher-Kelly
7Kenneth D Gadow
8Grace Gathungu
9Charles E Robertson
10Diana Ir
11Daniel N Frank
12Ellen Li
titleComparison of Fecal Microbiota in Children with Autism Spectrum Disorders and Neurotypical Siblings in the Simons Simplex Collection
description

In order to assess potential associations between autism spectrum disorder (ASD) phenotype, functional GI disorders and fecal microbiota, we recruited simplex families, which had only a single ASD proband and neurotypical (NT) siblings, through the Simons Simplex Community at the Interactive Autism Network (SSC@IAN). Fecal samples and metadata related to functional GI disorders and diet were collected from ASD probands and NT siblings of ASD probands (age 7-14). Functional gastrointestinal disorders (FGID) were assessed using the parent-completed ROME III questionnaire for pediatric FGIDs, and problem behaviors were assessed using the Child Behavior Check List (CBCL). Targeted quantitative polymerase chain reaction (qPCR) assays were conducted on selected taxa implicated in ASD, including Sutterella spp., Bacteroidetes spp. and Prevotella spp. Illumina sequencing of the V1V2 and the V1V3 regions of the bacterial 16S rRNA genes from fecal DNA was performed to an average depth of 208,000 and 107,000 high-quality reads respectively. Twenty-five of 59 ASD children and 13 of 44 NT siblings met ROME III criteria for at least one FGID. Functional constipation was more prevalent in ASD (17 of 59) compared to NT siblings (6 of 44, P = 0.035). The mean CBCL scores in NT siblings with FGID, ASD children with FGID and ASD without FGID were comparably higher (58-62 vs. 44, P < 0.0001) when compared to NT children without FGID. There was no significant difference in macronutrient intake between ASD and NT siblings. There was no significant difference in ASD severity scores between ASD children with and without FGID. No significant difference in diversity or overall microbial composition was detected between ASD children with NT siblings. Exploratory analysis of the 16S rRNA sequencing data, however, identified several low abundance taxa binned at the genus level that were associated with ASD and/or first order ASD*FGID interactions (FDR <0.1).

subjectSciences (General)
general
0English
1Public Library of Science (PLoS)
210.1371/journal.pone.0137725
3Directory of Open Access Journals (DOAJ)
sourceiddoaj_s
recordiddoaj_soai_doaj_org_article_76c2ad09a4bd41098a98237d17604e9b
issn
01932-6203
119326203
rsrctypearticle
creationdate2015
addtitlePLoS ONE
searchscope
0doaj_full
1doaj1
scope
0doaj_full
1doaj1
lsr45$$EView_full_text_in_DOAJ
tmp01Directory of Open Access Journals (DOAJ)
tmp02DOA
startdate20150101
enddate20150101
lsr40PLoS ONE, 01 January 2015, Vol.10 (10), p.e0137725
doi10.1371/journal.pone.0137725
citationpf e0137725 vol 10 issue 10
lsr30VSR-Enriched:[galeid, date, pqid, pages]
sort
titleComparison of Fecal Microbiota in Children with Autism Spectrum Disorders and Neurotypical Siblings in the Simons Simplex Collection
authorJoshua S Son ; Ling J Zheng ; Leahana M Rowehl ; Xinyu Tian ; Yuanhao Zhang ; Wei Zhu ; Leighann Litcher-Kelly ; Kenneth D Gadow ; Grace Gathungu ; Charles E Robertson ; Diana Ir ; Daniel N Frank ; Ellen Li
creationdate20150101
lso0120150101
facets
frbrgroupid6592141902433059015
frbrtype5
newrecords20190821
languageeng
topicSciences (General)
collectionDirectory of Open Access Journals (DOAJ)
prefilterarticles
rsrctypearticles
creatorcontrib
0Joshua S Son
1Ling J Zheng
2Leahana M Rowehl
3Xinyu Tian
4Yuanhao Zhang
5Wei Zhu
6Leighann Litcher-Kelly
7Kenneth D Gadow
8Grace Gathungu
9Charles E Robertson
10Diana Ir
11Daniel N Frank
12Ellen Li
jtitlePLoS ONE
creationdate2015
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext_linktorsrc
addata
au
0Joshua S Son
1Ling J Zheng
2Leahana M Rowehl
3Xinyu Tian
4Yuanhao Zhang
5Wei Zhu
6Leighann Litcher-Kelly
7Kenneth D Gadow
8Grace Gathungu
9Charles E Robertson
10Diana Ir
11Daniel N Frank
12Ellen Li
atitleComparison of Fecal Microbiota in Children with Autism Spectrum Disorders and Neurotypical Siblings in the Simons Simplex Collection
jtitlePLoS ONE
risdate20150101
volume10
issue10
spagee0137725
eissn1932-6203
formatjournal
genrearticle
ristypeJOUR
abstract

In order to assess potential associations between autism spectrum disorder (ASD) phenotype, functional GI disorders and fecal microbiota, we recruited simplex families, which had only a single ASD proband and neurotypical (NT) siblings, through the Simons Simplex Community at the Interactive Autism Network (SSC@IAN). Fecal samples and metadata related to functional GI disorders and diet were collected from ASD probands and NT siblings of ASD probands (age 7-14). Functional gastrointestinal disorders (FGID) were assessed using the parent-completed ROME III questionnaire for pediatric FGIDs, and problem behaviors were assessed using the Child Behavior Check List (CBCL). Targeted quantitative polymerase chain reaction (qPCR) assays were conducted on selected taxa implicated in ASD, including Sutterella spp., Bacteroidetes spp. and Prevotella spp. Illumina sequencing of the V1V2 and the V1V3 regions of the bacterial 16S rRNA genes from fecal DNA was performed to an average depth of 208,000 and 107,000 high-quality reads respectively. Twenty-five of 59 ASD children and 13 of 44 NT siblings met ROME III criteria for at least one FGID. Functional constipation was more prevalent in ASD (17 of 59) compared to NT siblings (6 of 44, P = 0.035). The mean CBCL scores in NT siblings with FGID, ASD children with FGID and ASD without FGID were comparably higher (58-62 vs. 44, P < 0.0001) when compared to NT children without FGID. There was no significant difference in macronutrient intake between ASD and NT siblings. There was no significant difference in ASD severity scores between ASD children with and without FGID. No significant difference in diversity or overall microbial composition was detected between ASD children with NT siblings. Exploratory analysis of the 16S rRNA sequencing data, however, identified several low abundance taxa binned at the genus level that were associated with ASD and/or first order ASD*FGID interactions (FDR <0.1).

pubPublic Library of Science (PLoS)
doi10.1371/journal.pone.0137725
urlhttps://doaj.org/article/76c2ad09a4bd41098a98237d17604e9b
lad01PLoS ONE
oafree_for_read
pagese0137725
date2015-10-01