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Characterization of Crude Extract of Mucor pusillus Rennin

The research preparation involved production of rennin, determination of optimum pH of crude extract of rennin, determination of optimum temperature of crude extract of rennin, and determination of enzyme kinetics. The obtained data was analyzed by descriptive analysis. Data was explained and elabor... Full description

Journal Title: Jurnal Ilmu dan Teknologi Hasil Ternak 01 February 2012, Vol.3(2), pp.1-7
Main Author: Khothibul Umam Al Awwaly
Other Authors: Mustakim Mustakim , Rachmat Agus Budiutomo
Format: Electronic Article Electronic Article
Language: ind
Quelle: Directory of Open Access Journals (DOAJ)
ID: ISSN: 1978-0303 ; E-ISSN: 2338-1620
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recordid: doaj_soai_doaj_org_article_9960ff3cf1834a439d12d7acf374f28d
title: Characterization of Crude Extract of Mucor pusillus Rennin
format: Article
creator:
  • Khothibul Umam Al Awwaly
  • Mustakim Mustakim
  • Rachmat Agus Budiutomo
ispartof: Jurnal Ilmu dan Teknologi Hasil Ternak, 01 February 2012, Vol.3(2), pp.1-7
description: The research preparation involved production of rennin, determination of optimum pH of crude extract of rennin, determination of optimum temperature of crude extract of rennin, and determination of enzyme kinetics. The obtained data was analyzed by descriptive analysis. Data was explained and elaborated according to science or new knowledge.  The result from characterization of rennin enzyme of Mucor pusillus showed that optimum enzyme reaction at pH 6.0, temperature at 32oC and concentration of substrate optimum at 2%. Each activity of proteolitic enzyme of Mucor pusillus at pH, temperature and concentration of substrate were: 0.09599 unit/ml, 0.06942 unit/ml, and 0.07577 unit/ml, respectively. The obtained value of Vmax and Km were 0.0836 Unit/ml and 0.356, respectively. Conclusion of this research was crude extract of M. pusillus rennin had the optimum pH at 6,0, optimum of temperature at 32oC and optimum of substrat consentration at 2%.   Keywords: rennin, Mucor pusillus, pH, temperature
language: ind
source: Directory of Open Access Journals (DOAJ)
identifier: ISSN: 1978-0303 ; E-ISSN: 2338-1620
fulltext: fulltext_linktorsrc
issn:
  • 1978-0303
  • 19780303
  • 2338-1620
  • 23381620
url: Link


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titleCharacterization of Crude Extract of Mucor pusillus Rennin
creatorKhothibul Umam Al Awwaly ; Mustakim Mustakim ; Rachmat Agus Budiutomo
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identifierISSN: 1978-0303 ; E-ISSN: 2338-1620
descriptionThe research preparation involved production of rennin, determination of optimum pH of crude extract of rennin, determination of optimum temperature of crude extract of rennin, and determination of enzyme kinetics. The obtained data was analyzed by descriptive analysis. Data was explained and elaborated according to science or new knowledge.  The result from characterization of rennin enzyme of Mucor pusillus showed that optimum enzyme reaction at pH 6.0, temperature at 32oC and concentration of substrate optimum at 2%. Each activity of proteolitic enzyme of Mucor pusillus at pH, temperature and concentration of substrate were: 0.09599 unit/ml, 0.06942 unit/ml, and 0.07577 unit/ml, respectively. The obtained value of Vmax and Km were 0.0836 Unit/ml and 0.356, respectively. Conclusion of this research was crude extract of M. pusillus rennin had the optimum pH at 6,0, optimum of temperature at 32oC and optimum of substrat consentration at 2%.   Keywords: rennin, Mucor pusillus, pH, temperature
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The research preparation involved production of rennin, determination of optimum pH of crude extract of rennin, determination of optimum temperature of crude extract of rennin, and determination of enzyme kinetics. The obtained data was analyzed by descriptive analysis. Data was explained and elaborated according to science or new knowledge.  The result from characterization of rennin enzyme of Mucor pusillus showed that optimum enzyme reaction at pH 6.0, temperature at 32oC and concentration of substrate optimum at 2%. Each activity of proteolitic enzyme of Mucor pusillus at pH, temperature and concentration of substrate were: 0.09599 unit/ml, 0.06942 unit/ml, and 0.07577 unit/ml, respectively. The obtained value of Vmax and Km were 0.0836 Unit/ml and 0.356, respectively. Conclusion of this research was crude extract of M. pusillus rennin had the optimum pH at 6,0, optimum of temperature at 32oC and optimum of substrat consentration at 2%.   Keywords: rennin, Mucor pusillus, pH, temperature

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The research preparation involved production of rennin, determination of optimum pH of crude extract of rennin, determination of optimum temperature of crude extract of rennin, and determination of enzyme kinetics. The obtained data was analyzed by descriptive analysis. Data was explained and elaborated according to science or new knowledge.  The result from characterization of rennin enzyme of Mucor pusillus showed that optimum enzyme reaction at pH 6.0, temperature at 32oC and concentration of substrate optimum at 2%. Each activity of proteolitic enzyme of Mucor pusillus at pH, temperature and concentration of substrate were: 0.09599 unit/ml, 0.06942 unit/ml, and 0.07577 unit/ml, respectively. The obtained value of Vmax and Km were 0.0836 Unit/ml and 0.356, respectively. Conclusion of this research was crude extract of M. pusillus rennin had the optimum pH at 6,0, optimum of temperature at 32oC and optimum of substrat consentration at 2%.   Keywords: rennin, Mucor pusillus, pH, temperature

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date2012-02-01