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Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams

This study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine,... Full description

Journal Title: PLoS ONE 01 January 2015, Vol.10(8), p.e0136865
Main Author: Ana Patrícia C Silva
Other Authors: Auricélio A Macêdo , Luciana F Costa , Cláudia E Rocha , Luize N N Garcia , Jade R D Farias , Priscilla P R Gomes , Gustavo C Teixeira , Kessler W J Fonseca , Andréa R F Maia , Gabriela G Neves , Everton L Romão , Teane M A Silva , Juliana P S Mol , Renata M Oliveira , Márcio S S Araújo , Ernane F Nascimento , Olindo A Martins-Filho , Humberto M Brandão , Tatiane A Paixão , Renato L Santos
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0136865
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title: Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams
format: Article
creator:
  • Ana Patrícia C Silva
  • Auricélio A Macêdo
  • Luciana F Costa
  • Cláudia E Rocha
  • Luize N N Garcia
  • Jade R D Farias
  • Priscilla P R Gomes
  • Gustavo C Teixeira
  • Kessler W J Fonseca
  • Andréa R F Maia
  • Gabriela G Neves
  • Everton L Romão
  • Teane M A Silva
  • Juliana P S Mol
  • Renata M Oliveira
  • Márcio S S Araújo
  • Ernane F Nascimento
  • Olindo A Martins-Filho
  • Humberto M Brandão
  • Tatiane A Paixão
  • Renato L Santos
subjects:
  • Sciences (General)
ispartof: PLoS ONE, 01 January 2015, Vol.10(8), p.e0136865
description: This study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis ΔabcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis ΔabcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0136865
fulltext: fulltext_linktorsrc
issn:
  • 1932-6203
  • 19326203
url: Link


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titleEncapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams
creatorAna Patrícia C Silva ; Auricélio A Macêdo ; Luciana F Costa ; Cláudia E Rocha ; Luize N N Garcia ; Jade R D Farias ; Priscilla P R Gomes ; Gustavo C Teixeira ; Kessler W J Fonseca ; Andréa R F Maia ; Gabriela G Neves ; Everton L Romão ; Teane M A Silva ; Juliana P S Mol ; Renata M Oliveira ; Márcio S S Araújo ; Ernane F Nascimento ; Olindo A Martins-Filho ; Humberto M Brandão ; Tatiane A Paixão ; Renato L Santos
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identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0136865
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descriptionThis study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis ΔabcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis ΔabcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams.
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titleEncapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams
description

This study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis ΔabcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis ΔabcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams.

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titleEncapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams
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abstract

This study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis ΔabcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis ΔabcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams.

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