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Application of culture and polymerase chain reaction (PCR) methods for isolation and identification ofMycoplasma synoviae on broiler chicken farms

Mycoplasma synoviae (M. synoviae) is a major worldwide poultry pathogen that causes serious economiclosses in the poultry industry. This study was designed to detect M. synoviae through culture isolation andpolymerase chain reaction (PCR) assay to demonstrated the involvement of M. synoviae infectio... Full description

Journal Title: Archives of Razi Institute 01 December 2011, Vol.66(2), pp.87-94
Main Author: Abtin, A.R
Other Authors: Ashtari, A , Homayounimehr, A.R , Pourbakhsh, S.A , Bayatzadeh, M.A
Format: Electronic Article Electronic Article
Language: English
Subjects:
Pcr
Quelle: Directory of Open Access Journals (DOAJ)
ID: ISSN: 0365-3439 ; E-ISSN: 2008-9872
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recordid: doaj_soai_doaj_org_article_efb8b29b8718410f893522e143abc321
title: Application of culture and polymerase chain reaction (PCR) methods for isolation and identification ofMycoplasma synoviae on broiler chicken farms
format: Article
creator:
  • Abtin, A.R
  • Ashtari, A
  • Homayounimehr, A.R
  • Pourbakhsh, S.A
  • Bayatzadeh, M.A
subjects:
  • Mycoplasma Synoviae
  • Broiler Chicken
  • Pcr
  • 16s Rrna
  • Culture
ispartof: Archives of Razi Institute, 01 December 2011, Vol.66(2), pp.87-94
description: Mycoplasma synoviae (M. synoviae) is a major worldwide poultry pathogen that causes serious economiclosses in the poultry industry. This study was designed to detect M. synoviae through culture isolation andpolymerase chain reaction (PCR) assay to demonstrated the involvement of M. synoviae infection in tracheaand the lung/air sac samples taken from commercial broiler chicken farms in 3 main provinces of Iran(Tehran, Markazi and Qazvin), with clinical signs of the disease. Total of 43 samples were cultured inPPLO broth media supplemented for M. synoviae isolation. The bacteria DNAs were extracted byphenol/chloroform method and the PCR assay amplifying the conserved region of 16S rRNA gene wasapplied for the detection of Mycoplasma genus in 163bp fragment and M. synoviae in 207bp fragment fromculture as same as in clinical samples. Of the 43 swabs 28(65.1%) yielded one of the potentially pathogenicmycoplasmas evaluated for using PPLO agar culture diagnostic method, and 33(76.8%) yielded one of thepotentially pathogenic Mycoplasmas evaluated for using Mycoplasma genus PCR as diagnostic method, and24(55.9%) of the swabs yielded M. synoviae for using M. synoviae PCR as diagnostic method. In this studywe had observed the highest quantity of M. synoviae infections in broiler chicken with PCR test. In conclusion, PCR is a more rapid, effective, sensitive and inexpensive method than the standard culture technique, that could be used as an alternative method for traditional culture and showed the real number of the M. synoviae contaminated broiler chicken farms.
language: eng
source: Directory of Open Access Journals (DOAJ)
identifier: ISSN: 0365-3439 ; E-ISSN: 2008-9872
fulltext: fulltext_linktorsrc
issn:
  • 0365-3439
  • 03653439
  • 2008-9872
  • 20089872
url: Link


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titleApplication of culture and polymerase chain reaction (PCR) methods for isolation and identification ofMycoplasma synoviae on broiler chicken farms
creatorAbtin, A.R ; Ashtari, A ; Homayounimehr, A.R ; Pourbakhsh, S.A ; Bayatzadeh, M.A
ispartofArchives of Razi Institute, 01 December 2011, Vol.66(2), pp.87-94
identifierISSN: 0365-3439 ; E-ISSN: 2008-9872
subjectMycoplasma Synoviae ; Broiler Chicken ; Pcr ; 16s Rrna ; Culture
descriptionMycoplasma synoviae (M. synoviae) is a major worldwide poultry pathogen that causes serious economiclosses in the poultry industry. This study was designed to detect M. synoviae through culture isolation andpolymerase chain reaction (PCR) assay to demonstrated the involvement of M. synoviae infection in tracheaand the lung/air sac samples taken from commercial broiler chicken farms in 3 main provinces of Iran(Tehran, Markazi and Qazvin), with clinical signs of the disease. Total of 43 samples were cultured inPPLO broth media supplemented for M. synoviae isolation. The bacteria DNAs were extracted byphenol/chloroform method and the PCR assay amplifying the conserved region of 16S rRNA gene wasapplied for the detection of Mycoplasma genus in 163bp fragment and M. synoviae in 207bp fragment fromculture as same as in clinical samples. Of the 43 swabs 28(65.1%) yielded one of the potentially pathogenicmycoplasmas evaluated for using PPLO agar culture diagnostic method, and 33(76.8%) yielded one of thepotentially pathogenic Mycoplasmas evaluated for using Mycoplasma genus PCR as diagnostic method, and24(55.9%) of the swabs yielded M. synoviae for using M. synoviae PCR as diagnostic method. In this studywe had observed the highest quantity of M. synoviae infections in broiler chicken with PCR test. In conclusion, PCR is a more rapid, effective, sensitive and inexpensive method than the standard culture technique, that could be used as an alternative method for traditional culture and showed the real number of the M. synoviae contaminated broiler chicken farms.
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titleApplication of culture and polymerase chain reaction (PCR) methods for isolation and identification ofMycoplasma synoviae on broiler chicken farms
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Mycoplasma synoviae (M. synoviae) is a major worldwide poultry pathogen that causes serious economiclosses in the poultry industry. This study was designed to detect M. synoviae through culture isolation andpolymerase chain reaction (PCR) assay to demonstrated the involvement of M. synoviae infection in tracheaand the lung/air sac samples taken from commercial broiler chicken farms in 3 main provinces of Iran(Tehran, Markazi and Qazvin), with clinical signs of the disease. Total of 43 samples were cultured inPPLO broth media supplemented for M. synoviae isolation. The bacteria DNAs were extracted byphenol/chloroform method and the PCR assay amplifying the conserved region of 16S rRNA gene wasapplied for the detection of Mycoplasma genus in 163bp fragment and M. synoviae in 207bp fragment fromculture as same as in clinical samples. Of the 43 swabs 28(65.1%) yielded one of the potentially pathogenicmycoplasmas evaluated for using PPLO agar culture diagnostic method, and 33(76.8%) yielded one of thepotentially pathogenic Mycoplasmas evaluated for using Mycoplasma genus PCR as diagnostic method, and24(55.9%) of the swabs yielded M. synoviae for using M. synoviae PCR as diagnostic method. In this studywe had observed the highest quantity of M. synoviae infections in broiler chicken with PCR test. In conclusion, PCR is a more rapid, effective, sensitive and inexpensive method than the standard culture technique, that could be used as an alternative method for traditional culture and showed the real number of the M. synoviae contaminated broiler chicken farms.

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Mycoplasma synoviae (M. synoviae) is a major worldwide poultry pathogen that causes serious economiclosses in the poultry industry. This study was designed to detect M. synoviae through culture isolation andpolymerase chain reaction (PCR) assay to demonstrated the involvement of M. synoviae infection in tracheaand the lung/air sac samples taken from commercial broiler chicken farms in 3 main provinces of Iran(Tehran, Markazi and Qazvin), with clinical signs of the disease. Total of 43 samples were cultured inPPLO broth media supplemented for M. synoviae isolation. The bacteria DNAs were extracted byphenol/chloroform method and the PCR assay amplifying the conserved region of 16S rRNA gene wasapplied for the detection of Mycoplasma genus in 163bp fragment and M. synoviae in 207bp fragment fromculture as same as in clinical samples. Of the 43 swabs 28(65.1%) yielded one of the potentially pathogenicmycoplasmas evaluated for using PPLO agar culture diagnostic method, and 33(76.8%) yielded one of thepotentially pathogenic Mycoplasmas evaluated for using Mycoplasma genus PCR as diagnostic method, and24(55.9%) of the swabs yielded M. synoviae for using M. synoviae PCR as diagnostic method. In this studywe had observed the highest quantity of M. synoviae infections in broiler chicken with PCR test. In conclusion, PCR is a more rapid, effective, sensitive and inexpensive method than the standard culture technique, that could be used as an alternative method for traditional culture and showed the real number of the M. synoviae contaminated broiler chicken farms.

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