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Diagnosis of porcine reproductive and respiratory syndrome using infected alveolar macrophages collected from live pigs

A highly sensitive method of detecting infection of live pigs with porcine reproductive and respiratory syndrome virus (PRRSV) was developed by testing alveolar macrophages collected by pulmonary lavage. Five pigs were exposed by oronasal inoculation or by contact to PRRSV when they were 10 (1 pig)... Full description

Journal Title: Veterinary microbiology 1996, Vol.49(1), pp.105-115
Main Author: Mengeling, William L
Other Authors: Vorwald, Ann C , Lager, Kelly M , Brockmeier, Susan L
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0378-1135 ; E-ISSN: 1873-2542 ; DOI: 10.1016/0378-1135(95)00173-5
Link: http://dx.doi.org/10.1016/0378-1135(95)00173-5
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recordid: elsevier_sdoi_10_1016_0378_1135_95_00173_5
title: Diagnosis of porcine reproductive and respiratory syndrome using infected alveolar macrophages collected from live pigs
format: Article
creator:
  • Mengeling, William L
  • Vorwald, Ann C
  • Lager, Kelly M
  • Brockmeier, Susan L
subjects:
  • Porcine Reproductive and Respiratory Syndrome Virus
  • Pig Rrsv
  • Alveolar Macrophages
  • Pulmonary Lavage
  • Diagnosis
  • Porcine Reproductive and Respiratory Syndrome Virus
  • Pig Rrsv
  • Alveolar Macrophages
  • Pulmonary Lavage
  • Diagnosis
  • Biology
  • Veterinary Medicine
ispartof: Veterinary microbiology, 1996, Vol.49(1), pp.105-115
description: A highly sensitive method of detecting infection of live pigs with porcine reproductive and respiratory syndrome virus (PRRSV) was developed by testing alveolar macrophages collected by pulmonary lavage. Five pigs were exposed by oronasal inoculation or by contact to PRRSV when they were 10 (1 pig) or 14 weeks (4 pigs) of age. Diagnostic samples (alveolar macrophages and sera) were collected from each pig just before exposure to PRRSV. During the next 9 weeks sera were collected at weekly intervals and alveolar macrophages were collected at weeks 2 and 4–9. Both sera and alveolar macrophages were suitable for detecting early infection, but alveolar macrophages were clearly the better sample after longer intervals. Virus was last isolated from serum at week 4 (from 1 of 5 pigs), whereas it was isolated from the alveolar macrophages of 4 of the 5 pigs at week 4 and from at least 2 pigs at each of the weekly intervals thereafter (i.e. weeks 5, 6, 7, 8, and 9 postexposure). The most...
language: eng
source:
identifier: ISSN: 0378-1135 ; E-ISSN: 1873-2542 ; DOI: 10.1016/0378-1135(95)00173-5
fulltext: fulltext
issn:
  • 0378-1135
  • 03781135
  • 1873-2542
  • 18732542
url: Link


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subjectPorcine Reproductive and Respiratory Syndrome Virus ; Pig Rrsv ; Alveolar Macrophages ; Pulmonary Lavage ; Diagnosis ; Porcine Reproductive and Respiratory Syndrome Virus ; Pig Rrsv ; Alveolar Macrophages ; Pulmonary Lavage ; Diagnosis ; Biology ; Veterinary Medicine
descriptionA highly sensitive method of detecting infection of live pigs with porcine reproductive and respiratory syndrome virus (PRRSV) was developed by testing alveolar macrophages collected by pulmonary lavage. Five pigs were exposed by oronasal inoculation or by contact to PRRSV when they were 10 (1 pig) or 14 weeks (4 pigs) of age. Diagnostic samples (alveolar macrophages and sera) were collected from each pig just before exposure to PRRSV. During the next 9 weeks sera were collected at weekly intervals and alveolar macrophages were collected at weeks 2 and 4–9. Both sera and alveolar macrophages were suitable for detecting early infection, but alveolar macrophages were clearly the better sample after longer intervals. Virus was last isolated from serum at week 4 (from 1 of 5 pigs), whereas it was isolated from the alveolar macrophages of 4 of the 5 pigs at week 4 and from at least 2 pigs at each of the weekly intervals thereafter (i.e. weeks 5, 6, 7, 8, and 9 postexposure). The most...
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A highly sensitive method of detecting infection of live pigs with porcine reproductive and respiratory syndrome virus (PRRSV) was developed by testing alveolar macrophages collected by pulmonary lavage. Five pigs were exposed by oronasal inoculation or by contact to PRRSV when they were 10 (1 pig) or 14 weeks (4 pigs) of age. Diagnostic samples (alveolar macrophages and sera) were collected from each pig just before exposure to PRRSV. During the next 9 weeks sera were collected at weekly intervals and alveolar macrophages were collected at weeks 2 and 4–9. Both sera and alveolar macrophages were suitable for detecting early infection, but alveolar macrophages were clearly the better sample after longer intervals. Virus was last isolated from serum at week 4 (from 1 of 5 pigs), whereas it was isolated from the alveolar macrophages of 4 of the 5 pigs at week 4 and from at least 2 pigs at each of the weekly intervals thereafter (i.e. weeks 5, 6, 7, 8, and 9 postexposure). The most...

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