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Diagnosis of porcine reproductive and respiratory syndrome using infected alveolar macrophages collected from live pigs

A highly sensitive method of detecting infection of live pigs with porcine reproductive and respiratory syndrome virus (PRRSV) was developed by testing alveolar macrophages collected by pulmonary lavage. Five pigs were exposed by oronasal inoculation or by contact to PRRSV when they were 10 (1 pig)... Full description

Journal Title: Veterinary microbiology 1996, Vol.49 (1), p.105-115
Main Author: Mengeling, William L
Other Authors: Vorwald, Ann C , Lager, Kelly M , Brockmeier, Susan L
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Amsterdam: Elsevier B.V
ID: ISSN: 0378-1135
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recordid: cdi_proquest_miscellaneous_78430174
title: Diagnosis of porcine reproductive and respiratory syndrome using infected alveolar macrophages collected from live pigs
format: Article
creator:
  • Mengeling, William L
  • Vorwald, Ann C
  • Lager, Kelly M
  • Brockmeier, Susan L
subjects:
  • Alveolar macrophages
  • Animal viral diseases
  • Animals
  • Biological and medical sciences
  • Bronchoalveolar Lavage - methods
  • Bronchoalveolar Lavage - veterinary
  • Bronchoalveolar Lavage Fluid
  • Cell Line
  • CERDO
  • DIAGNOSIS
  • DIAGNOSTIC
  • DIAGNOSTICO
  • ENFERMEDADES RESPIRATORIAS
  • Infectious diseases
  • Lung - pathology
  • Lung - virology
  • MACROFAGOS
  • MACROPHAGE
  • MACROPHAGES
  • Macrophages, Alveolar - pathology
  • Macrophages, Alveolar - virology
  • MALADIE RESPIRATOIRE
  • Medical sciences
  • Pig RRSV
  • PORCIN
  • Porcine Reproductive and Respiratory Syndrome - blood
  • Porcine Reproductive and Respiratory Syndrome - diagnosis
  • Porcine Reproductive and Respiratory Syndrome - pathology
  • Porcine reproductive and respiratory syndrome virus
  • Porcine respiratory and reproductive syndrome virus - isolation & purification
  • Pulmonary lavage
  • RESPIRATORY DISEASES
  • Sensitivity and Specificity
  • SINTOMAS
  • SWINE
  • SYMPTOME
  • SYMPTOMS
  • Viral diseases
ispartof: Veterinary microbiology, 1996, Vol.49 (1), p.105-115
description: A highly sensitive method of detecting infection of live pigs with porcine reproductive and respiratory syndrome virus (PRRSV) was developed by testing alveolar macrophages collected by pulmonary lavage. Five pigs were exposed by oronasal inoculation or by contact to PRRSV when they were 10 (1 pig) or 14 weeks (4 pigs) of age. Diagnostic samples (alveolar macrophages and sera) were collected from each pig just before exposure to PRRSV. During the next 9 weeks sera were collected at weekly intervals and alveolar macrophages were collected at weeks 2 and 4–9. Both sera and alveolar macrophages were suitable for detecting early infection, but alveolar macrophages were clearly the better sample after longer intervals. Virus was last isolated from serum at week 4 (from 1 of 5 pigs), whereas it was isolated from the alveolar macrophages of 4 of the 5 pigs at week 4 and from at least 2 pigs at each of the weekly intervals thereafter (i.e. weeks 5, 6, 7, 8, and 9 postexposure). The most sensitive method of testing alveolar macrophages for PRRSV was cocultivation with MARC-145 cells. None of the pigs had any clinical signs after exposure to PRRSV or as a result of pulmonary lavage and there was no evidence that repeated pulmonary lavage caused anything other than a mild, transient (mild hyperemia) tissue reaction.
language: eng
source:
identifier: ISSN: 0378-1135
fulltext: no_fulltext
issn:
  • 0378-1135
  • 1873-2542
url: Link


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titleDiagnosis of porcine reproductive and respiratory syndrome using infected alveolar macrophages collected from live pigs
creatorMengeling, William L ; Vorwald, Ann C ; Lager, Kelly M ; Brockmeier, Susan L
creatorcontribMengeling, William L ; Vorwald, Ann C ; Lager, Kelly M ; Brockmeier, Susan L
descriptionA highly sensitive method of detecting infection of live pigs with porcine reproductive and respiratory syndrome virus (PRRSV) was developed by testing alveolar macrophages collected by pulmonary lavage. Five pigs were exposed by oronasal inoculation or by contact to PRRSV when they were 10 (1 pig) or 14 weeks (4 pigs) of age. Diagnostic samples (alveolar macrophages and sera) were collected from each pig just before exposure to PRRSV. During the next 9 weeks sera were collected at weekly intervals and alveolar macrophages were collected at weeks 2 and 4–9. Both sera and alveolar macrophages were suitable for detecting early infection, but alveolar macrophages were clearly the better sample after longer intervals. Virus was last isolated from serum at week 4 (from 1 of 5 pigs), whereas it was isolated from the alveolar macrophages of 4 of the 5 pigs at week 4 and from at least 2 pigs at each of the weekly intervals thereafter (i.e. weeks 5, 6, 7, 8, and 9 postexposure). The most sensitive method of testing alveolar macrophages for PRRSV was cocultivation with MARC-145 cells. None of the pigs had any clinical signs after exposure to PRRSV or as a result of pulmonary lavage and there was no evidence that repeated pulmonary lavage caused anything other than a mild, transient (mild hyperemia) tissue reaction.
identifier
0ISSN: 0378-1135
1EISSN: 1873-2542
2DOI: 10.1016/0378-1135(95)00173-5
3PMID: 8861647
4CODEN: VMICDQ
languageeng
publisherAmsterdam: Elsevier B.V
subjectAlveolar macrophages ; Animal viral diseases ; Animals ; Biological and medical sciences ; Bronchoalveolar Lavage - methods ; Bronchoalveolar Lavage - veterinary ; Bronchoalveolar Lavage Fluid ; Cell Line ; CERDO ; DIAGNOSIS ; DIAGNOSTIC ; DIAGNOSTICO ; ENFERMEDADES RESPIRATORIAS ; Infectious diseases ; Lung - pathology ; Lung - virology ; MACROFAGOS ; MACROPHAGE ; MACROPHAGES ; Macrophages, Alveolar - pathology ; Macrophages, Alveolar - virology ; MALADIE RESPIRATOIRE ; Medical sciences ; Pig RRSV ; PORCIN ; Porcine Reproductive and Respiratory Syndrome - blood ; Porcine Reproductive and Respiratory Syndrome - diagnosis ; Porcine Reproductive and Respiratory Syndrome - pathology ; Porcine reproductive and respiratory syndrome virus ; Porcine respiratory and reproductive syndrome virus - isolation & purification ; Pulmonary lavage ; RESPIRATORY DISEASES ; Sensitivity and Specificity ; SINTOMAS ; SWINE ; SYMPTOME ; SYMPTOMS ; Viral diseases
ispartofVeterinary microbiology, 1996, Vol.49 (1), p.105-115
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1Vorwald, Ann C
2Lager, Kelly M
3Brockmeier, Susan L
title
0Diagnosis of porcine reproductive and respiratory syndrome using infected alveolar macrophages collected from live pigs
1Veterinary microbiology
addtitleVet Microbiol
descriptionA highly sensitive method of detecting infection of live pigs with porcine reproductive and respiratory syndrome virus (PRRSV) was developed by testing alveolar macrophages collected by pulmonary lavage. Five pigs were exposed by oronasal inoculation or by contact to PRRSV when they were 10 (1 pig) or 14 weeks (4 pigs) of age. Diagnostic samples (alveolar macrophages and sera) were collected from each pig just before exposure to PRRSV. During the next 9 weeks sera were collected at weekly intervals and alveolar macrophages were collected at weeks 2 and 4–9. Both sera and alveolar macrophages were suitable for detecting early infection, but alveolar macrophages were clearly the better sample after longer intervals. Virus was last isolated from serum at week 4 (from 1 of 5 pigs), whereas it was isolated from the alveolar macrophages of 4 of the 5 pigs at week 4 and from at least 2 pigs at each of the weekly intervals thereafter (i.e. weeks 5, 6, 7, 8, and 9 postexposure). The most sensitive method of testing alveolar macrophages for PRRSV was cocultivation with MARC-145 cells. None of the pigs had any clinical signs after exposure to PRRSV or as a result of pulmonary lavage and there was no evidence that repeated pulmonary lavage caused anything other than a mild, transient (mild hyperemia) tissue reaction.
subject
0Alveolar macrophages
1Animal viral diseases
2Animals
3Biological and medical sciences
4Bronchoalveolar Lavage - methods
5Bronchoalveolar Lavage - veterinary
6Bronchoalveolar Lavage Fluid
7Cell Line
8CERDO
9DIAGNOSIS
10DIAGNOSTIC
11DIAGNOSTICO
12ENFERMEDADES RESPIRATORIAS
13Infectious diseases
14Lung - pathology
15Lung - virology
16MACROFAGOS
17MACROPHAGE
18MACROPHAGES
19Macrophages, Alveolar - pathology
20Macrophages, Alveolar - virology
21MALADIE RESPIRATOIRE
22Medical sciences
23Pig RRSV
24PORCIN
25Porcine Reproductive and Respiratory Syndrome - blood
26Porcine Reproductive and Respiratory Syndrome - diagnosis
27Porcine Reproductive and Respiratory Syndrome - pathology
28Porcine reproductive and respiratory syndrome virus
29Porcine respiratory and reproductive syndrome virus - isolation & purification
30Pulmonary lavage
31RESPIRATORY DISEASES
32Sensitivity and Specificity
33SINTOMAS
34SWINE
35SYMPTOME
36SYMPTOMS
37Viral diseases
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authorMengeling, William L ; Vorwald, Ann C ; Lager, Kelly M ; Brockmeier, Susan L
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0Alveolar macrophages
1Animal viral diseases
2Animals
3Biological and medical sciences
4Bronchoalveolar Lavage - methods
5Bronchoalveolar Lavage - veterinary
6Bronchoalveolar Lavage Fluid
7Cell Line
8CERDO
9DIAGNOSIS
10DIAGNOSTIC
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13Infectious diseases
14Lung - pathology
15Lung - virology
16MACROFAGOS
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18MACROPHAGES
19Macrophages, Alveolar - pathology
20Macrophages, Alveolar - virology
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22Medical sciences
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24PORCIN
25Porcine Reproductive and Respiratory Syndrome - blood
26Porcine Reproductive and Respiratory Syndrome - diagnosis
27Porcine Reproductive and Respiratory Syndrome - pathology
28Porcine reproductive and respiratory syndrome virus
29Porcine respiratory and reproductive syndrome virus - isolation & purification
30Pulmonary lavage
31RESPIRATORY DISEASES
32Sensitivity and Specificity
33SINTOMAS
34SWINE
35SYMPTOME
36SYMPTOMS
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abstractA highly sensitive method of detecting infection of live pigs with porcine reproductive and respiratory syndrome virus (PRRSV) was developed by testing alveolar macrophages collected by pulmonary lavage. Five pigs were exposed by oronasal inoculation or by contact to PRRSV when they were 10 (1 pig) or 14 weeks (4 pigs) of age. Diagnostic samples (alveolar macrophages and sera) were collected from each pig just before exposure to PRRSV. During the next 9 weeks sera were collected at weekly intervals and alveolar macrophages were collected at weeks 2 and 4–9. Both sera and alveolar macrophages were suitable for detecting early infection, but alveolar macrophages were clearly the better sample after longer intervals. Virus was last isolated from serum at week 4 (from 1 of 5 pigs), whereas it was isolated from the alveolar macrophages of 4 of the 5 pigs at week 4 and from at least 2 pigs at each of the weekly intervals thereafter (i.e. weeks 5, 6, 7, 8, and 9 postexposure). The most sensitive method of testing alveolar macrophages for PRRSV was cocultivation with MARC-145 cells. None of the pigs had any clinical signs after exposure to PRRSV or as a result of pulmonary lavage and there was no evidence that repeated pulmonary lavage caused anything other than a mild, transient (mild hyperemia) tissue reaction.
copAmsterdam
pubElsevier B.V
pmid8861647
doi10.1016/0378-1135(95)00173-5