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Liquid chromatographic determination of levamisole in animal plasma: ultraviolet versus tandem mass spectrometric detection

This work presents the optimization and validation of a liquid chromatography (LC)-ultraviolet (UV) detection method and a tandem mass spectrometry (LC-MS/MS) method with positive electrospray ionization for the determination of levamisole, an anthelmintic for veterinary use, in animal plasma. A liq... Full description

Journal Title: Analytica Chimica Acta 2003, Vol.483(1), pp.215-224
Main Author: De Baere, Siegrid
Other Authors: Cherlet, Marc , Croubels, Siska , Baert, Kris , De Backer, Patrick
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0003-2670 ; E-ISSN: 1873-4324 ; DOI: 10.1016/S0003-2670(02)01377-6
Link: https://www.sciencedirect.com/science/article/pii/S0003267002013776
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title: Liquid chromatographic determination of levamisole in animal plasma: ultraviolet versus tandem mass spectrometric detection
format: Article
creator:
  • De Baere, Siegrid
  • Cherlet, Marc
  • Croubels, Siska
  • Baert, Kris
  • De Backer, Patrick
subjects:
  • Levamisole
  • Anthelmintic
  • Animal Plasma
  • Liquid Chromatography-Ultraviolet
  • Liquid Chromatography–Mass Spectrometry/MS
  • Tandem Mass Spectrometry
  • Validation
  • Pharmacokinetics
  • Residue Analysis
  • Chemistry
ispartof: Analytica Chimica Acta, 2003, Vol.483(1), pp.215-224
description: This work presents the optimization and validation of a liquid chromatography (LC)-ultraviolet (UV) detection method and a tandem mass spectrometry (LC-MS/MS) method with positive electrospray ionization for the determination of levamisole, an anthelmintic for veterinary use, in animal plasma. A liquid-liquid extraction procedure in alkaline medium, using hexane- isoamyl alcohol (95:5, v/v) as extraction solvent, was performed to clean-up the plasma samples prior to LC-UV analysis. The sample preparation prior to LC-MS/MS analysis consisted of a protein precipitation step, using acetonitrile. Methyllevamisole was used as the internal standard. Chromatographic separation was achieved on a LiChrospher 60 RP-select B (5 mu m) column using a mixture of 0.1 M ammonia acetate in water and acetonitrile as the mobile phase. A gradient (flow rate 1 ml min super(-1)) or isocratic (flow rate 0.2 ml min super(-1)) elution was performed in case of the LC-UV and LC-MS/MS methods,...
language: eng
source:
identifier: ISSN: 0003-2670 ; E-ISSN: 1873-4324 ; DOI: 10.1016/S0003-2670(02)01377-6
fulltext: fulltext
issn:
  • 0003-2670
  • 00032670
  • 1873-4324
  • 18734324
url: Link


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titleLiquid chromatographic determination of levamisole in animal plasma: ultraviolet versus tandem mass spectrometric detection
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subjectLevamisole ; Anthelmintic ; Animal Plasma ; Liquid Chromatography-Ultraviolet ; Liquid Chromatography–Mass Spectrometry/MS ; Tandem Mass Spectrometry ; Validation ; Pharmacokinetics ; Residue Analysis ; Chemistry
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descriptionThis work presents the optimization and validation of a liquid chromatography (LC)-ultraviolet (UV) detection method and a tandem mass spectrometry (LC-MS/MS) method with positive electrospray ionization for the determination of levamisole, an anthelmintic for veterinary use, in animal plasma. A liquid-liquid extraction procedure in alkaline medium, using hexane- isoamyl alcohol (95:5, v/v) as extraction solvent, was performed to clean-up the plasma samples prior to LC-UV analysis. The sample preparation prior to LC-MS/MS analysis consisted of a protein precipitation step, using acetonitrile. Methyllevamisole was used as the internal standard. Chromatographic separation was achieved on a LiChrospher 60 RP-select B (5 mu m) column using a mixture of 0.1 M ammonia acetate in water and acetonitrile as the mobile phase. A gradient (flow rate 1 ml min super(-1)) or isocratic (flow rate 0.2 ml min super(-1)) elution was performed in case of the LC-UV and LC-MS/MS methods,...
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