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Sequencing and analysis of a 63 kb bacterial artificial chromosome insert from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi

Wolbachia endosymbiotic bacteria are widespread in filarial nematodes and are directly involved in the immune response of the host. In addition, antibiotics which disrupt Wolbachia interfere with filarial nematode development thus, Wolbachia provide an excellent target for control of filariasis. A 6... Full description

Journal Title: International Journal for Parasitology February 2002, Vol.32(2), pp.159-166
Main Author: Ware, Jennifer
Other Authors: Moran, Laurie , Foster, Jeremy , Posfai, Janos , Vincze, Tamas , Guiliano, David , Blaxter, Mark , Eisen, Jonathan , Slatko, Barton
Format: Electronic Article Electronic Article
Language: English
Subjects:
Bac
ID: ISSN: 0020-7519 ; E-ISSN: 1879-0135 ; DOI: 10.1016/S0020-7519(01)00367-8
Link: https://www.sciencedirect.com/science/article/pii/S0020751901003678
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recordid: elsevier_sdoi_10_1016_S0020_7519_01_00367_8
title: Sequencing and analysis of a 63 kb bacterial artificial chromosome insert from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi
format: Article
creator:
  • Ware, Jennifer
  • Moran, Laurie
  • Foster, Jeremy
  • Posfai, Janos
  • Vincze, Tamas
  • Guiliano, David
  • Blaxter, Mark
  • Eisen, Jonathan
  • Slatko, Barton
subjects:
  • DNA Sequencing
  • Wolbachia
  • Bac
  • Brugia Malayi
  • Filarial Parasite
  • Transposon
  • Biology
  • Zoology
ispartof: International Journal for Parasitology, February 2002, Vol.32(2), pp.159-166
description: Wolbachia endosymbiotic bacteria are widespread in filarial nematodes and are directly involved in the immune response of the host. In addition, antibiotics which disrupt Wolbachia interfere with filarial nematode development thus, Wolbachia provide an excellent target for control of filariasis. A 63.1 kb bacterial artificial chromosome insert, from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi, has been sequenced using the New England Biolabs Inc. Genome Priming System() transposition kit in conjunction with primer walking methods. The bacterial artificial chromosome insert contains approximately 57 potential ORFs which have been compared by individual protein BLAST analysis with the 35 published complete microbial genomes in the Comprehensive Microbial Resource database at The Institute for Genomic Research and in the NCBI GenBank database, as well as to data from 22 incomplete genomes from the DOE Joint Genome Institute. Twenty five of the putative ORFs have significant similarity to genes from the alpha-proteobacteria Rickettsia prowazekii, the most closely related completed genome, as well as to the newly sequenced alpha-proteobacteria endosymbiont Sinorhizobium meliloti. The bacterial artificial chromosome insert sequence however has little conserved synteny with the R. prowazekii and S. meliloti genomes. Significant sequence similarity was also found in comparisons with the currently available sequence data from the Wolbachia endosymbiont of Drosophila melanogaster. Analysis of this bacterial artificial chromosome insert provides useful gene density and comparative genomic data that will contribute to whole genome sequencing of Wolbachia from the B. malayi host. This will also lead to a better understanding of the interactions between the endosymbiont and its host and will offer novel approaches and drug targets for elimination of filarial disease.
language: eng
source:
identifier: ISSN: 0020-7519 ; E-ISSN: 1879-0135 ; DOI: 10.1016/S0020-7519(01)00367-8
fulltext: fulltext
issn:
  • 0020-7519
  • 00207519
  • 1879-0135
  • 18790135
url: Link


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titleSequencing and analysis of a 63 kb bacterial artificial chromosome insert from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi
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ispartofInternational Journal for Parasitology, February 2002, Vol.32(2), pp.159-166
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descriptionWolbachia endosymbiotic bacteria are widespread in filarial nematodes and are directly involved in the immune response of the host. In addition, antibiotics which disrupt Wolbachia interfere with filarial nematode development thus, Wolbachia provide an excellent target for control of filariasis. A 63.1 kb bacterial artificial chromosome insert, from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi, has been sequenced using the New England Biolabs Inc. Genome Priming System() transposition kit in conjunction with primer walking methods. The bacterial artificial chromosome insert contains approximately 57 potential ORFs which have been compared by individual protein BLAST analysis with the 35 published complete microbial genomes in the Comprehensive Microbial Resource database at The Institute for Genomic Research and in the NCBI GenBank database, as well as to data from 22 incomplete genomes from the DOE Joint Genome Institute. Twenty five of the putative ORFs have significant similarity to genes from the alpha-proteobacteria Rickettsia prowazekii, the most closely related completed genome, as well as to the newly sequenced alpha-proteobacteria endosymbiont Sinorhizobium meliloti. The bacterial artificial chromosome insert sequence however has little conserved synteny with the R. prowazekii and S. meliloti genomes. Significant sequence similarity was also found in comparisons with the currently available sequence data from the Wolbachia endosymbiont of Drosophila melanogaster. Analysis of this bacterial artificial chromosome insert provides useful gene density and comparative genomic data that will contribute to whole genome sequencing of Wolbachia from the B. malayi host. This will also lead to a better understanding of the interactions between the endosymbiont and its host and will offer novel approaches and drug targets for elimination of filarial disease.
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