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Effect of temperature modulation and bvg mutation of Bordetella bronchiseptica on adhesion, intracellular survival and cytotoxicity for swine alveolar macrophages

Bordetella bronchiseptica causes respiratory disease in swine, yet there are no studies examining the interaction of B. bronchiseptica with swine alveolar macrophages. A swine isolate of B. bronchiseptica was able to adhere to, and survive intracellularly in, swine alveolar macrophages, but the rela... Full description

Journal Title: Veterinary microbiology 2000, Vol.73 (1), p.1-12
Main Author: Brockmeier, Susan L
Other Authors: Register, Karen B
Format: Electronic Article Electronic Article
Language: English
Subjects:
Pig
Publisher: Netherlands: Elsevier B.V
ID: ISSN: 0378-1135
Link: https://www.ncbi.nlm.nih.gov/pubmed/10731613
Zum Text:
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title: Effect of temperature modulation and bvg mutation of Bordetella bronchiseptica on adhesion, intracellular survival and cytotoxicity for swine alveolar macrophages
format: Article
creator:
  • Brockmeier, Susan L
  • Register, Karen B
subjects:
  • Adhesion
  • Alveolar macrophages
  • Animals
  • Antibodies, Bacterial - biosynthesis
  • Bacterial Adhesion - immunology
  • Bacterial Outer Membrane Proteins - immunology
  • Bacterial Proteins - genetics
  • Benzenesulfonates - chemistry
  • Bordetella bronchiseptica
  • Bordetella bronchiseptica - genetics
  • Bordetella bronchiseptica - immunology
  • Bordetella bronchiseptica - pathogenicity
  • Bordetella Infections - immunology
  • Bordetella Infections - microbiology
  • Bordetella Infections - veterinary
  • Cells, Cultured
  • Cytochalasin D - chemistry
  • Cytotoxicity
  • Fluorescent Dyes - chemistry
  • Gene Expression Regulation
  • Immunoblotting - veterinary
  • Intracellular survival
  • Macrophages, Alveolar - immunology
  • Macrophages, Alveolar - microbiology
  • Mutation
  • Nucleic Acid Synthesis Inhibitors - chemistry
  • Pig
  • Polymyxin B - chemistry
  • Rabbits
  • Swine
  • Swine Diseases - immunology
  • Swine Diseases - microbiology
  • Temperature
  • Transcription Factors - genetics
  • Virulence
ispartof: Veterinary microbiology, 2000, Vol.73 (1), p.1-12
description: Bordetella bronchiseptica causes respiratory disease in swine, yet there are no studies examining the interaction of B. bronchiseptica with swine alveolar macrophages. A swine isolate of B. bronchiseptica was able to adhere to, and survive intracellularly in, swine alveolar macrophages, but the relative ability of the bacteria to accomplish these functions was dependent on its phenotypic phase and culture conditions. More bacteria were observed extracellularly as well as intracellularly by immunofluorescent staining when B. bronchiseptica was cultured at 23°C as compared to 37°C. However, more bacteria cultured at 37°C were found surviving intracellularly after the macrophages were cultured with polymyxin B to kill extracellular bacteria. Similar results were seen in experiments performed with an isogenic Bvg − phase-locked mutant of B. bronchiseptica cultured at 37 or 23°C, indicating that another temperature dependent mechanism in addition to bvg may play a role in adhesion and intracellular survival . B. bronchiseptica was cytotoxic for swine alveolar macrophages in the Bvg + phase only. The cytotoxicity of B. bronchiseptica for alveolar macrophages, and its ability to survive phagocytosis, are no doubt important to escape from immune clearance mechanisms and establish infection, and could leave the host susceptible to secondary respiratory pathogens.
language: eng
source:
identifier: ISSN: 0378-1135
fulltext: no_fulltext
issn:
  • 0378-1135
  • 1873-2542
url: Link


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titleEffect of temperature modulation and bvg mutation of Bordetella bronchiseptica on adhesion, intracellular survival and cytotoxicity for swine alveolar macrophages
creatorBrockmeier, Susan L ; Register, Karen B
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descriptionBordetella bronchiseptica causes respiratory disease in swine, yet there are no studies examining the interaction of B. bronchiseptica with swine alveolar macrophages. A swine isolate of B. bronchiseptica was able to adhere to, and survive intracellularly in, swine alveolar macrophages, but the relative ability of the bacteria to accomplish these functions was dependent on its phenotypic phase and culture conditions. More bacteria were observed extracellularly as well as intracellularly by immunofluorescent staining when B. bronchiseptica was cultured at 23°C as compared to 37°C. However, more bacteria cultured at 37°C were found surviving intracellularly after the macrophages were cultured with polymyxin B to kill extracellular bacteria. Similar results were seen in experiments performed with an isogenic Bvg − phase-locked mutant of B. bronchiseptica cultured at 37 or 23°C, indicating that another temperature dependent mechanism in addition to bvg may play a role in adhesion and intracellular survival . B. bronchiseptica was cytotoxic for swine alveolar macrophages in the Bvg + phase only. The cytotoxicity of B. bronchiseptica for alveolar macrophages, and its ability to survive phagocytosis, are no doubt important to escape from immune clearance mechanisms and establish infection, and could leave the host susceptible to secondary respiratory pathogens.
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1EISSN: 1873-2542
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languageeng
publisherNetherlands: Elsevier B.V
subjectAdhesion ; Alveolar macrophages ; Animals ; Antibodies, Bacterial - biosynthesis ; Bacterial Adhesion - immunology ; Bacterial Outer Membrane Proteins - immunology ; Bacterial Proteins - genetics ; Benzenesulfonates - chemistry ; Bordetella bronchiseptica ; Bordetella bronchiseptica - genetics ; Bordetella bronchiseptica - immunology ; Bordetella bronchiseptica - pathogenicity ; Bordetella Infections - immunology ; Bordetella Infections - microbiology ; Bordetella Infections - veterinary ; Cells, Cultured ; Cytochalasin D - chemistry ; Cytotoxicity ; Fluorescent Dyes - chemistry ; Gene Expression Regulation ; Immunoblotting - veterinary ; Intracellular survival ; Macrophages, Alveolar - immunology ; Macrophages, Alveolar - microbiology ; Mutation ; Nucleic Acid Synthesis Inhibitors - chemistry ; Pig ; Polymyxin B - chemistry ; Rabbits ; Swine ; Swine Diseases - immunology ; Swine Diseases - microbiology ; Temperature ; Transcription Factors - genetics ; Virulence
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0Effect of temperature modulation and bvg mutation of Bordetella bronchiseptica on adhesion, intracellular survival and cytotoxicity for swine alveolar macrophages
1Veterinary microbiology
addtitleVet Microbiol
descriptionBordetella bronchiseptica causes respiratory disease in swine, yet there are no studies examining the interaction of B. bronchiseptica with swine alveolar macrophages. A swine isolate of B. bronchiseptica was able to adhere to, and survive intracellularly in, swine alveolar macrophages, but the relative ability of the bacteria to accomplish these functions was dependent on its phenotypic phase and culture conditions. More bacteria were observed extracellularly as well as intracellularly by immunofluorescent staining when B. bronchiseptica was cultured at 23°C as compared to 37°C. However, more bacteria cultured at 37°C were found surviving intracellularly after the macrophages were cultured with polymyxin B to kill extracellular bacteria. Similar results were seen in experiments performed with an isogenic Bvg − phase-locked mutant of B. bronchiseptica cultured at 37 or 23°C, indicating that another temperature dependent mechanism in addition to bvg may play a role in adhesion and intracellular survival . B. bronchiseptica was cytotoxic for swine alveolar macrophages in the Bvg + phase only. The cytotoxicity of B. bronchiseptica for alveolar macrophages, and its ability to survive phagocytosis, are no doubt important to escape from immune clearance mechanisms and establish infection, and could leave the host susceptible to secondary respiratory pathogens.
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1Alveolar macrophages
2Animals
3Antibodies, Bacterial - biosynthesis
4Bacterial Adhesion - immunology
5Bacterial Outer Membrane Proteins - immunology
6Bacterial Proteins - genetics
7Benzenesulfonates - chemistry
8Bordetella bronchiseptica
9Bordetella bronchiseptica - genetics
10Bordetella bronchiseptica - immunology
11Bordetella bronchiseptica - pathogenicity
12Bordetella Infections - immunology
13Bordetella Infections - microbiology
14Bordetella Infections - veterinary
15Cells, Cultured
16Cytochalasin D - chemistry
17Cytotoxicity
18Fluorescent Dyes - chemistry
19Gene Expression Regulation
20Immunoblotting - veterinary
21Intracellular survival
22Macrophages, Alveolar - immunology
23Macrophages, Alveolar - microbiology
24Mutation
25Nucleic Acid Synthesis Inhibitors - chemistry
26Pig
27Polymyxin B - chemistry
28Rabbits
29Swine
30Swine Diseases - immunology
31Swine Diseases - microbiology
32Temperature
33Transcription Factors - genetics
34Virulence
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titleEffect of temperature modulation and bvg mutation of Bordetella bronchiseptica on adhesion, intracellular survival and cytotoxicity for swine alveolar macrophages
authorBrockmeier, Susan L ; Register, Karen B
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1Alveolar macrophages
2Animals
3Antibodies, Bacterial - biosynthesis
4Bacterial Adhesion - immunology
5Bacterial Outer Membrane Proteins - immunology
6Bacterial Proteins - genetics
7Benzenesulfonates - chemistry
8Bordetella bronchiseptica
9Bordetella bronchiseptica - genetics
10Bordetella bronchiseptica - immunology
11Bordetella bronchiseptica - pathogenicity
12Bordetella Infections - immunology
13Bordetella Infections - microbiology
14Bordetella Infections - veterinary
15Cells, Cultured
16Cytochalasin D - chemistry
17Cytotoxicity
18Fluorescent Dyes - chemistry
19Gene Expression Regulation
20Immunoblotting - veterinary
21Intracellular survival
22Macrophages, Alveolar - immunology
23Macrophages, Alveolar - microbiology
24Mutation
25Nucleic Acid Synthesis Inhibitors - chemistry
26Pig
27Polymyxin B - chemistry
28Rabbits
29Swine
30Swine Diseases - immunology
31Swine Diseases - microbiology
32Temperature
33Transcription Factors - genetics
34Virulence
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atitleEffect of temperature modulation and bvg mutation of Bordetella bronchiseptica on adhesion, intracellular survival and cytotoxicity for swine alveolar macrophages
jtitleVeterinary microbiology
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date2000
risdate2000
volume73
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pages1-12
issn0378-1135
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abstractBordetella bronchiseptica causes respiratory disease in swine, yet there are no studies examining the interaction of B. bronchiseptica with swine alveolar macrophages. A swine isolate of B. bronchiseptica was able to adhere to, and survive intracellularly in, swine alveolar macrophages, but the relative ability of the bacteria to accomplish these functions was dependent on its phenotypic phase and culture conditions. More bacteria were observed extracellularly as well as intracellularly by immunofluorescent staining when B. bronchiseptica was cultured at 23°C as compared to 37°C. However, more bacteria cultured at 37°C were found surviving intracellularly after the macrophages were cultured with polymyxin B to kill extracellular bacteria. Similar results were seen in experiments performed with an isogenic Bvg − phase-locked mutant of B. bronchiseptica cultured at 37 or 23°C, indicating that another temperature dependent mechanism in addition to bvg may play a role in adhesion and intracellular survival . B. bronchiseptica was cytotoxic for swine alveolar macrophages in the Bvg + phase only. The cytotoxicity of B. bronchiseptica for alveolar macrophages, and its ability to survive phagocytosis, are no doubt important to escape from immune clearance mechanisms and establish infection, and could leave the host susceptible to secondary respiratory pathogens.
copNetherlands
pubElsevier B.V
pmid10731613
doi10.1016/S0378-1135(99)00201-1