schliessen

Filtern

 

Bibliotheken

Effect of temperature modulation and bvg mutation of Bordetella bronchiseptica on adhesion, intracellular survival and cytotoxicity for swine alveolar macrophages

Bordetella bronchiseptica causes respiratory disease in swine, yet there are no studies examining the interaction of B. bronchiseptica with swine alveolar macrophages. A swine isolate of B. bronchiseptica was able to adhere to, and survive intracellularly in, swine alveolar macrophages, but the rela... Full description

Journal Title: Veterinary Microbiology 2000, Vol.73(1), pp.1-12
Main Author: Brockmeier, Susan L
Other Authors: Register, Karen B
Format: Electronic Article Electronic Article
Language: English
Subjects:
Pig
ID: ISSN: 0378-1135 ; E-ISSN: 1873-2542 ; DOI: 10.1016/S0378-1135(99)00201-1
Link: https://www.sciencedirect.com/science/article/pii/S0378113599002011
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: elsevier_sdoi_10_1016_S0378_1135_99_00201_1
title: Effect of temperature modulation and bvg mutation of Bordetella bronchiseptica on adhesion, intracellular survival and cytotoxicity for swine alveolar macrophages
format: Article
creator:
  • Brockmeier, Susan L
  • Register, Karen B
subjects:
  • Bordetella Bronchiseptica
  • Pig
  • Alveolar Macrophages
  • Adhesion
  • Intracellular Survival
  • Cytotoxicity
  • Biology
  • Veterinary Medicine
ispartof: Veterinary Microbiology, 2000, Vol.73(1), pp.1-12
description: Bordetella bronchiseptica causes respiratory disease in swine, yet there are no studies examining the interaction of B. bronchiseptica with swine alveolar macrophages. A swine isolate of B. bronchiseptica was able to adhere to, and survive intracellularly in, swine alveolar macrophages, but the relative ability of the bacteria to accomplish these functions was dependent on its phenotypic phase and culture conditions. More bacteria were observed extracellularly as well as intracellularly by immunofluorescent staining when B. bronchiseptica was cultured at 23 degree C as compared to 37 degree C. However, more bacteria cultured at 37 degree C were found surviving intracellularly after the macrophages were cultured with polymyxin B to kill extracellular bacteria. Similar results were seen in experiments performed with an isogenic Bvg super(-) phase-locked mutant of B. bronchiseptica cultured at 37 or 23 degree C, indicating that another temperature dependent mechanism in addition to bvg may play a role in adhesion and intracellular survival. B. bronchiseptica was cytotoxic for swine alveolar macrophages in the Bvg super(+) phase only. The cytotoxicity of B. bronchiseptica for alveolar macrophages, and its ability to survive phagocytosis, are no doubt important to escape from immune clearance mechanisms and establish infection, and could leave the host susceptible to secondary respiratory pathogens.
language: eng
source:
identifier: ISSN: 0378-1135 ; E-ISSN: 1873-2542 ; DOI: 10.1016/S0378-1135(99)00201-1
fulltext: fulltext
issn:
  • 0378-1135
  • 03781135
  • 1873-2542
  • 18732542
url: Link


@attributes
ID1147421627
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordiddoi_10_1016_S0378_1135_99_00201_1
sourceidelsevier_s
recordidTN_elsevier_sdoi_10_1016_S0378_1135_99_00201_1
sourcesystemPC
dbid
0--K
1--M
2.~1
31B1
41RT
51~.
6457
74G.
87-5
98P~
109JM
11AABNK
12AAEDT
13AAKOC
14AALCJ
15AAOAW
16AAQFI
17AARKO
18AATLK
19ABBQC
20ABFNM
21ABKYH
22ABMZM
23ABRWV
24ABYKQ
25ACDAQ
26ACIUM
27ACRLP
28ADQTV
29AEKER
30AEQOU
31AEXOQ
32AFKWA
33AFTJW
34AFXIZ
35AGEKW
36AGHFR
37AGUBO
38AGYEJ
39AHHHB
40AIKHN
41AITUG
42AJBFU
43AJOXV
44AJRQY
45AMFUW
46ANZVX
47BLXMC
48BNPGV
49CBWCG
50CJTIS
51EO8
52EO9
53EP2
54EP3
55FDB
56FGOYB
57FIRID
58FNPLU
59G-Q
60GBLVA
61HLV
62HMG
63J1W
64KOM
65LCYCR
66LUGTX
67OAUVE
68P-8
69P-9
70PC.
71Q38
72QYZTP
73R2-
74RPZ
75SAB
76SDF
77SDG
78SDP
79SES
80SEW
81SIN
82SNL
83SSA
84SSH
85SSI
86SSZ
87SVS
88T5K
89~G-
pqid17510863
display
typearticle
titleEffect of temperature modulation and bvg mutation of Bordetella bronchiseptica on adhesion, intracellular survival and cytotoxicity for swine alveolar macrophages
creatorBrockmeier, Susan L ; Register, Karen B
ispartofVeterinary Microbiology, 2000, Vol.73(1), pp.1-12
identifier
subjectBordetella Bronchiseptica ; Pig ; Alveolar Macrophages ; Adhesion ; Intracellular Survival ; Cytotoxicity ; Biology ; Veterinary Medicine
languageeng
source
descriptionBordetella bronchiseptica causes respiratory disease in swine, yet there are no studies examining the interaction of B. bronchiseptica with swine alveolar macrophages. A swine isolate of B. bronchiseptica was able to adhere to, and survive intracellularly in, swine alveolar macrophages, but the relative ability of the bacteria to accomplish these functions was dependent on its phenotypic phase and culture conditions. More bacteria were observed extracellularly as well as intracellularly by immunofluorescent staining when B. bronchiseptica was cultured at 23 degree C as compared to 37 degree C. However, more bacteria cultured at 37 degree C were found surviving intracellularly after the macrophages were cultured with polymyxin B to kill extracellular bacteria. Similar results were seen in experiments performed with an isogenic Bvg super(-) phase-locked mutant of B. bronchiseptica cultured at 37 or 23 degree C, indicating that another temperature dependent mechanism in addition to bvg may play a role in adhesion and intracellular survival. B. bronchiseptica was cytotoxic for swine alveolar macrophages in the Bvg super(+) phase only. The cytotoxicity of B. bronchiseptica for alveolar macrophages, and its ability to survive phagocytosis, are no doubt important to escape from immune clearance mechanisms and establish infection, and could leave the host susceptible to secondary respiratory pathogens.
version5
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttps://www.sciencedirect.com/science/article/pii/S0378113599002011$$EView_record_in_ScienceDirect_(Access_to_full_text_may_be_restricted)
search
creatorcontrib
0Brockmeier, Susan L
1Register, Karen B
titleEffect of temperature modulation and bvg mutation of Bordetella bronchiseptica on adhesion, intracellular survival and cytotoxicity for swine alveolar macrophages
subject
0Bordetella Bronchiseptica
1Pig
2Alveolar Macrophages
3Adhesion
4Intracellular Survival
5Cytotoxicity
6Biology
7Veterinary Medicine
general
0English
1Elsevier B.V
210.1016/S0378-1135(99)00201-1
3ScienceDirect (Elsevier)
4ScienceDirect Journals (Elsevier)
sourceidelsevier_s
recordidelsevier_sdoi_10_1016_S0378_1135_99_00201_1
issn
00378-1135
103781135
21873-2542
318732542
rsrctypearticle
creationdate2000
addtitleVeterinary Microbiology
searchscope
0elsevier_full
1elsevier2
scope
0elsevier_full
1elsevier2
lsr44$$EView_record_in_ScienceDirect_(Access_to_full_text_may_be_restricted)
tmp01ScienceDirect Journals (Elsevier)
tmp02
0--K
1--M
2.~1
31B1
41RT
51~.
6457
74G.
87-5
98P~
109JM
11AABNK
12AAEDT
13AAKOC
14AALCJ
15AAOAW
16AAQFI
17AARKO
18AATLK
19ABBQC
20ABFNM
21ABKYH
22ABMZM
23ABRWV
24ABYKQ
25ACDAQ
26ACIUM
27ACRLP
28ADQTV
29AEKER
30AEQOU
31AEXOQ
32AFKWA
33AFTJW
34AFXIZ
35AGEKW
36AGHFR
37AGUBO
38AGYEJ
39AHHHB
40AIKHN
41AITUG
42AJBFU
43AJOXV
44AJRQY
45AMFUW
46ANZVX
47BLXMC
48BNPGV
49CBWCG
50CJTIS
51EO8
52EO9
53EP2
54EP3
55FDB
56FGOYB
57FIRID
58FNPLU
59G-Q
60GBLVA
61HLV
62HMG
63J1W
64KOM
65LCYCR
66LUGTX
67OAUVE
68P-8
69P-9
70PC.
71Q38
72QYZTP
73R2-
74RPZ
75SAB
76SDF
77SDG
78SDP
79SES
80SEW
81SIN
82SNL
83SSA
84SSH
85SSI
86SSZ
87SVS
88T5K
89~G-
startdate20000101
enddate20001231
lsr40Veterinary Microbiology, 2000, Vol.73 (1), pp.1-12
doi10.1016/S0378-1135(99)00201-1
citationpf 1 pt 12 vol 73 issue 1
lsr30VSR-Enriched:[pqid, description]
sort
titleEffect of temperature modulation and bvg mutation of Bordetella bronchiseptica on adhesion, intracellular survival and cytotoxicity for swine alveolar macrophages
authorBrockmeier, Susan L ; Register, Karen B
creationdate20000000
lso0120000000
facets
frbrgroupid-657170883119317820
frbrtype5
newrecords20190904
languageeng
topic
0Bordetella Bronchiseptica
1Pig
2Alveolar Macrophages
3Adhesion
4Intracellular Survival
5Cytotoxicity
6Biology
7Veterinary Medicine
collectionScienceDirect (Elsevier)
prefilterarticles
rsrctypearticles
creatorcontrib
0Brockmeier, Susan L
1Register, Karen B
jtitleVeterinary Microbiology
creationdate2000
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Brockmeier
1Register
aufirst
0Susan L
1Karen B
auinitS
auinit1S
au
0Brockmeier, Susan L
1Register, Karen B
atitleEffect of temperature modulation and bvg mutation of Bordetella bronchiseptica on adhesion, intracellular survival and cytotoxicity for swine alveolar macrophages
jtitleVeterinary Microbiology
date2000
risdate2000
volume73
issue1
spage1
epage12
pages1-12
issn0378-1135
eissn1873-2542
formatjournal
genrearticle
ristypeJOUR
pubElsevier B.V
doi10.1016/S0378-1135(99)00201-1
lad01Veterinary Microbiology