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Lentiviral vector-mediated transduction of goat undifferentiated spermatogonia

Recent studies show that spermatogonial stem cells (SSCs) are able to colonize and form mature spermatozoa following transplantation into germ cell depleted testes of recipient males. Therefore, efficient ways for enrichment and gene transfer into SSCs provides a powerful tool for production of tran... Full description

Journal Title: Animal Reproduction Science December 2015, Vol.163, pp.10-17
Main Author: Abbasi, Hassan
Other Authors: Hosseini, Sayyed Morteza , Hajian, Mahdi , Nasiri, Zahra , Bahadorani, Mehrnoosh , Tahmoorespur, Mojtaba , Nasiri, Mohammad Reza , Nasr-Esfahani, Mohammad Hossein
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0378-4320 ; E-ISSN: 1873-2232 ; DOI: 10.1016/j.anireprosci.2015.09.002
Link: https://www.sciencedirect.com/science/article/pii/S0378432015300130
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recordid: elsevier_sdoi_10_1016_j_anireprosci_2015_09_002
title: Lentiviral vector-mediated transduction of goat undifferentiated spermatogonia
format: Article
creator:
  • Abbasi, Hassan
  • Hosseini, Sayyed Morteza
  • Hajian, Mahdi
  • Nasiri, Zahra
  • Bahadorani, Mehrnoosh
  • Tahmoorespur, Mojtaba
  • Nasiri, Mohammad Reza
  • Nasr-Esfahani, Mohammad Hossein
subjects:
  • Goat
  • Sscs
  • Lentiviral Vectors
  • MACS
  • Thy1
  • Transduction
  • Agriculture
  • Veterinary Medicine
  • Anatomy & Physiology
ispartof: Animal Reproduction Science, December 2015, Vol.163, pp.10-17
description: Recent studies show that spermatogonial stem cells (SSCs) are able to colonize and form mature spermatozoa following transplantation into germ cell depleted testes of recipient males. Therefore, efficient ways for enrichment and gene transfer into SSCs provides a powerful tool for production of transgenic animals. In order to adapt the technique to goats, three issues were addressed: (i) enrichment of the undifferentiated spermatogonia including SSCs using magnetic activated cell sorting (MACS), (ii) lentiviral vector-mediated transduction of an enhanced green fluorescent protein (EGFP) transgene into enriched cells, and (iii) transplantation of transduced undifferentiated spermatogonia into the germ cell depleted testes of immune-suppressed mice to assess for migration and colony formation ability. Enriched cells were transduced by lentiviral vectors and subsequently analyzed for expression of , , , ...
language: eng
source:
identifier: ISSN: 0378-4320 ; E-ISSN: 1873-2232 ; DOI: 10.1016/j.anireprosci.2015.09.002
fulltext: fulltext
issn:
  • 0378-4320
  • 03784320
  • 1873-2232
  • 18732232
url: Link


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titleLentiviral vector-mediated transduction of goat undifferentiated spermatogonia
creatorAbbasi, Hassan ; Hosseini, Sayyed Morteza ; Hajian, Mahdi ; Nasiri, Zahra ; Bahadorani, Mehrnoosh ; Tahmoorespur, Mojtaba ; Nasiri, Mohammad Reza ; Nasr-Esfahani, Mohammad Hossein
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descriptionRecent studies show that spermatogonial stem cells (SSCs) are able to colonize and form mature spermatozoa following transplantation into germ cell depleted testes of recipient males. Therefore, efficient ways for enrichment and gene transfer into SSCs provides a powerful tool for production of transgenic animals. In order to adapt the technique to goats, three issues were addressed: (i) enrichment of the undifferentiated spermatogonia including SSCs using magnetic activated cell sorting (MACS), (ii) lentiviral vector-mediated transduction of an enhanced green fluorescent protein (EGFP) transgene into enriched cells, and (iii) transplantation of transduced undifferentiated spermatogonia into the germ cell depleted testes of immune-suppressed mice to assess for migration and colony formation ability. Enriched cells were transduced by lentiviral vectors and subsequently analyzed for expression of , , , ...
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