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An interferon-beta promoter reporter assay for high throughput identification of compounds against multiple RNA viruses

•A cell-based IFN-β reporter assay was established for high throughput screen.•This assay can be applied to any virus that is able to activate IFN response in the report cells.•The assay allows for identification of compounds with antiviral and innate immune response modulating activity. Virus infec... Full description

Journal Title: Antiviral Research July 2014, Vol.107, pp.56-65
Main Author: Guo, Fang
Other Authors: Zhao, Xuesen , Gill, Tina , Zhou, Yan , Campagna, Matthew , Wang, Lijuan , Liu, Fei , Zhang, Pinghu , Dipaolo, Laura , Du, Yanming , Xu, Xiaodong , Jiang, Dong , Wei, Lai , Cuconati, Andrea , Block, Timothy M , Guo, Ju-Tao , Chang, Jinhong
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0166-3542 ; E-ISSN: 1872-9096 ; DOI: 10.1016/j.antiviral.2014.04.010
Link: http://dx.doi.org/10.1016/j.antiviral.2014.04.010
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recordid: elsevier_sdoi_10_1016_j_antiviral_2014_04_010
title: An interferon-beta promoter reporter assay for high throughput identification of compounds against multiple RNA viruses
format: Article
creator:
  • Guo, Fang
  • Zhao, Xuesen
  • Gill, Tina
  • Zhou, Yan
  • Campagna, Matthew
  • Wang, Lijuan
  • Liu, Fei
  • Zhang, Pinghu
  • Dipaolo, Laura
  • Du, Yanming
  • Xu, Xiaodong
  • Jiang, Dong
  • Wei, Lai
  • Cuconati, Andrea
  • Block, Timothy M
  • Guo, Ju-Tao
  • Chang, Jinhong
subjects:
  • High Throughput Assay
  • Antiviral
  • Innate Immune Modulator
  • Dengue Virus
  • High Throughput Assay
  • Antiviral
  • Innate Immune Modulator
  • Dengue Virus
  • Medicine
  • Biology
ispartof: Antiviral Research, July 2014, Vol.107, pp.56-65
description: •A cell-based IFN-β reporter assay was established for high throughput screen.•This assay can be applied to any virus that is able to activate IFN response in the report cells.•The assay allows for identification of compounds with antiviral and innate immune response modulating activity. Virus infection of host cells is sensed by innate pattern recognition receptors (PRRs) and induces production of type I interferons (IFNs) and other inflammatory cytokines. These cytokines orchestrate the elimination of the viruses but are occasionally detrimental to the hosts. The outcomes and pathogenesis of viral infection are largely determined by the specific interaction between the viruses and their host cells. Therefore, compounds that either inhibit viral infection or modulate virus-induced cytokine response should be considered as candidates for managing virus infection. The aim of the study was to identify compounds in both categories, using a single cell-based assay....
language: eng
source:
identifier: ISSN: 0166-3542 ; E-ISSN: 1872-9096 ; DOI: 10.1016/j.antiviral.2014.04.010
fulltext: fulltext
issn:
  • 0166-3542
  • 01663542
  • 1872-9096
  • 18729096
url: Link


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titleAn interferon-beta promoter reporter assay for high throughput identification of compounds against multiple RNA viruses
creatorGuo, Fang ; Zhao, Xuesen ; Gill, Tina ; Zhou, Yan ; Campagna, Matthew ; Wang, Lijuan ; Liu, Fei ; Zhang, Pinghu ; Dipaolo, Laura ; Du, Yanming ; Xu, Xiaodong ; Jiang, Dong ; Wei, Lai ; Cuconati, Andrea ; Block, Timothy M ; Guo, Ju-Tao ; Chang, Jinhong
ispartofAntiviral Research, July 2014, Vol.107, pp.56-65
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subjectHigh Throughput Assay ; Antiviral ; Innate Immune Modulator ; Dengue Virus ; High Throughput Assay ; Antiviral ; Innate Immune Modulator ; Dengue Virus ; Medicine ; Biology
description•A cell-based IFN-β reporter assay was established for high throughput screen.•This assay can be applied to any virus that is able to activate IFN response in the report cells.•The assay allows for identification of compounds with antiviral and innate immune response modulating activity. Virus infection of host cells is sensed by innate pattern recognition receptors (PRRs) and induces production of type I interferons (IFNs) and other inflammatory cytokines. These cytokines orchestrate the elimination of the viruses but are occasionally detrimental to the hosts. The outcomes and pathogenesis of viral infection are largely determined by the specific interaction between the viruses and their host cells. Therefore, compounds that either inhibit viral infection or modulate virus-induced cytokine response should be considered as candidates for managing virus infection. The aim of the study was to identify compounds in both categories, using a single cell-based assay....
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•A cell-based IFN-β reporter assay was established for high throughput screen.•This assay can be applied to any virus that is able to activate IFN response in the report cells.•The assay allows for identification of compounds with antiviral and innate immune response modulating activity.

Virus infection of host cells is sensed by innate pattern recognition receptors (PRRs) and induces production of type I interferons (IFNs) and other inflammatory cytokines. These cytokines orchestrate the elimination of the viruses but are occasionally detrimental to the hosts. The outcomes and pathogenesis of viral infection are largely determined by the specific interaction between the viruses and their host cells. Therefore, compounds that either inhibit viral infection or modulate virus-induced cytokine response should be considered as candidates for managing virus infection. The aim of the study was to identify compounds in both categories, using a single cell-based assay....

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authorGuo, Fang ; Zhao, Xuesen ; Gill, Tina ; Zhou, Yan ; Campagna, Matthew ; Wang, Lijuan ; Liu, Fei ; Zhang, Pinghu ; Dipaolo, Laura ; Du, Yanming ; Xu, Xiaodong ; Jiang, Dong ; Wei, Lai ; Cuconati, Andrea ; Block, Timothy M ; Guo, Ju-Tao ; Chang, Jinhong
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abstract

•A cell-based IFN-β reporter assay was established for high throughput screen.•This assay can be applied to any virus that is able to activate IFN response in the report cells.•The assay allows for identification of compounds with antiviral and innate immune response modulating activity.

Virus infection of host cells is sensed by innate pattern recognition receptors (PRRs) and induces production of type I interferons (IFNs) and other inflammatory cytokines. These cytokines orchestrate the elimination of the viruses but are occasionally detrimental to the hosts. The outcomes and pathogenesis of viral infection are largely determined by the specific interaction between the viruses and their host cells. Therefore, compounds that either inhibit viral infection or modulate virus-induced cytokine response should be considered as candidates for managing virus infection. The aim of the study was to identify compounds in both categories, using a single cell-based assay....

pubElsevier B.V
doi10.1016/j.antiviral.2014.04.010
lad01Antiviral Research
issue1
date2014-07