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Sperm cryopreservation of yellow drum Nibea albiflora: A special emphasis on post-thaw sperm quality

This study was intended to develop an optimal sperm cryopreservation protocol for yellow drum ( ), a commercially important fish species in East Asia. Specifically, we evaluated cryoprotectant type, concentration, and cooling rate and found that the highest fertilization (56–61%) and hatch (42–47%)... Full description

Journal Title: Aquaculture 24 November 2012, Vol.368-369, pp.82-88
Main Author: Dai, Tongren
Other Authors: Zhao, Enhui , Lu, Gang , Che, Kai , He, Qiutao , Lu, Yonglin , Fang, Qiongshan , Wang, Hansheng , Zheng, Leyun , Li, Suping , Huang, Changjiang , Dong, Qiaoxiang
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0044-8486 ; E-ISSN: 1873-5622 ; DOI: 10.1016/j.aquaculture.2012.09.017
Link: https://www.sciencedirect.com/science/article/pii/S0044848612005510
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recordid: elsevier_sdoi_10_1016_j_aquaculture_2012_09_017
title: Sperm cryopreservation of yellow drum Nibea albiflora: A special emphasis on post-thaw sperm quality
format: Article
creator:
  • Dai, Tongren
  • Zhao, Enhui
  • Lu, Gang
  • Che, Kai
  • He, Qiutao
  • Lu, Yonglin
  • Fang, Qiongshan
  • Wang, Hansheng
  • Zheng, Leyun
  • Li, Suping
  • Huang, Changjiang
  • Dong, Qiaoxiang
subjects:
  • Yellow Drum
  • Nibea Albiflora
  • Sperm Cryopreservation
  • Sperm Quality
  • Cholesterol
  • Sphingomyelin
  • Agriculture
ispartof: Aquaculture, 24 November 2012, Vol.368-369, pp.82-88
description: This study was intended to develop an optimal sperm cryopreservation protocol for yellow drum ( ), a commercially important fish species in East Asia. Specifically, we evaluated cryoprotectant type, concentration, and cooling rate and found that the highest fertilization (56–61%) and hatch (42–47%) can be obtained from yellow drum sperm suspended in 5% ethylene glycol or 10% dimethyl sulfoxide, cooled at 220 °C/min, thawed in 40 °C water bath for 7 s, and fertilized with fresh (non-frozen) eggs at a sperm-to-egg ratio of 10 . We also compared other sperm quality parameters such as sperm motility, motility duration, membrane integrity, mitochondrial membrane potential, and DNA fragmentation between fresh and thawed sperm samples. Although cryopreservation resulted in a significant increase in DNA fragmentation and significant decreases in the remaining parameters, the magnitude of these...
language: eng
source:
identifier: ISSN: 0044-8486 ; E-ISSN: 1873-5622 ; DOI: 10.1016/j.aquaculture.2012.09.017
fulltext: fulltext
issn:
  • 0044-8486
  • 00448486
  • 1873-5622
  • 18735622
url: Link


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titleSperm cryopreservation of yellow drum Nibea albiflora: A special emphasis on post-thaw sperm quality
creatorDai, Tongren ; Zhao, Enhui ; Lu, Gang ; Che, Kai ; He, Qiutao ; Lu, Yonglin ; Fang, Qiongshan ; Wang, Hansheng ; Zheng, Leyun ; Li, Suping ; Huang, Changjiang ; Dong, Qiaoxiang
ispartofAquaculture, 24 November 2012, Vol.368-369, pp.82-88
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subjectYellow Drum ; Nibea Albiflora ; Sperm Cryopreservation ; Sperm Quality ; Cholesterol ; Sphingomyelin ; Agriculture
descriptionThis study was intended to develop an optimal sperm cryopreservation protocol for yellow drum ( ), a commercially important fish species in East Asia. Specifically, we evaluated cryoprotectant type, concentration, and cooling rate and found that the highest fertilization (56–61%) and hatch (42–47%) can be obtained from yellow drum sperm suspended in 5% ethylene glycol or 10% dimethyl sulfoxide, cooled at 220 °C/min, thawed in 40 °C water bath for 7 s, and fertilized with fresh (non-frozen) eggs at a sperm-to-egg ratio of 10 . We also compared other sperm quality parameters such as sperm motility, motility duration, membrane integrity, mitochondrial membrane potential, and DNA fragmentation between fresh and thawed sperm samples. Although cryopreservation resulted in a significant increase in DNA fragmentation and significant decreases in the remaining parameters, the magnitude of these...
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