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Apoptosis of vascular smooth muscle cells induced by photodynamic therapy with protoporphyrin IX

Photodynamic therapy (PDT) had been shown effective in the treatment of intimal hyperplasia, which contributes to restenosis, by eradicating cells in the vessel wall. This study is designed to evaluate the effects of PDT with protoporphyrin IX (PpIX) on the viability of vascular smooth muscle cells... Full description

Journal Title: Biochemical and Biophysical Research Communications 01 January 2010, Vol.391(1), pp.69-72
Main Author: Li, Qingsong
Other Authors: Cheng, Jiali , Peng, Chenghai , Li, Zhitao , Shi, Sa , Liang, Huijuan , Tian, Ye , Zhang, Zhiguo , Cao, Wenwu
Format: Electronic Article Electronic Article
Language: English
Subjects:
Pdt
ID: ISSN: 0006-291X ; E-ISSN: 1090-2104 ; DOI: 10.1016/j.bbrc.2009.11.003
Link: https://www.sciencedirect.com/science/article/pii/S0006291X09021585
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recordid: elsevier_sdoi_10_1016_j_bbrc_2009_11_003
title: Apoptosis of vascular smooth muscle cells induced by photodynamic therapy with protoporphyrin IX
format: Article
creator:
  • Li, Qingsong
  • Cheng, Jiali
  • Peng, Chenghai
  • Li, Zhitao
  • Shi, Sa
  • Liang, Huijuan
  • Tian, Ye
  • Zhang, Zhiguo
  • Cao, Wenwu
subjects:
  • Pdt
  • Protoporphyrin IX
  • Smcs
  • Apoptosis
  • Biology
  • Chemistry
  • Anatomy & Physiology
ispartof: Biochemical and Biophysical Research Communications, 01 January 2010, Vol.391(1), pp.69-72
description: Photodynamic therapy (PDT) had been shown effective in the treatment of intimal hyperplasia, which contributes to restenosis, by eradicating cells in the vessel wall. This study is designed to evaluate the effects of PDT with protoporphyrin IX (PpIX) on the viability of vascular smooth muscle cells (SMCs) and to define the cell-death pathway. Fluorescence microscopy and laser-induced fluorescence spectroscopic detection showed that SMCs selectively uptake PpIX, and the intracellular PpIX concentration increases with the amount of PpIX in the incubation solution. PDT with PpIX impaired cellular viability from 93 ± 3.4% to 36 ± 3.9% when the light intensity increases from 2 to 9 J/cm and intracellular PpIX concentration increases from 0.5 to 20 μg/ml. Although PDT induced both apoptosis and necrosis, the ratio of apoptotic cells increased with light dosage or intracellular PpIX concentration. The loss of mitochondrial membrane potential coincided with the apoptotic ratio. Our results indicated that the induction of apoptosis of SMCs may be one of the mechanisms by which PDT inhibits restenosis .
language: eng
source:
identifier: ISSN: 0006-291X ; E-ISSN: 1090-2104 ; DOI: 10.1016/j.bbrc.2009.11.003
fulltext: fulltext
issn:
  • 0006-291X
  • 0006291X
  • 1090-2104
  • 10902104
url: Link


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titleApoptosis of vascular smooth muscle cells induced by photodynamic therapy with protoporphyrin IX
creatorLi, Qingsong ; Cheng, Jiali ; Peng, Chenghai ; Li, Zhitao ; Shi, Sa ; Liang, Huijuan ; Tian, Ye ; Zhang, Zhiguo ; Cao, Wenwu
ispartofBiochemical and Biophysical Research Communications, 01 January 2010, Vol.391(1), pp.69-72
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descriptionPhotodynamic therapy (PDT) had been shown effective in the treatment of intimal hyperplasia, which contributes to restenosis, by eradicating cells in the vessel wall. This study is designed to evaluate the effects of PDT with protoporphyrin IX (PpIX) on the viability of vascular smooth muscle cells (SMCs) and to define the cell-death pathway. Fluorescence microscopy and laser-induced fluorescence spectroscopic detection showed that SMCs selectively uptake PpIX, and the intracellular PpIX concentration increases with the amount of PpIX in the incubation solution. PDT with PpIX impaired cellular viability from 93 ± 3.4% to 36 ± 3.9% when the light intensity increases from 2 to 9 J/cm and intracellular PpIX concentration increases from 0.5 to 20 μg/ml. Although PDT induced both apoptosis and necrosis, the ratio of apoptotic cells increased with light dosage or intracellular PpIX concentration. The loss of mitochondrial membrane potential coincided with the apoptotic ratio. Our results indicated that the induction of apoptosis of SMCs may be one of the mechanisms by which PDT inhibits restenosis .
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