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Expression and proliferation profiles of PKC, JNK and p38MAPK in physiologically stretched human bladder smooth muscle cells

•Stretch induces proliferation in human bladder smooth muscle cells (HBSMC).•5% Equibiaxial elongation produces maximum proliferation.•Physiologic stretch decreases apoptotic cell death.•PKC is involved in functional modulation of bladder.•JNK and p38 are not involved in proliferating HBSMC. To dete... Full description

Journal Title: Biochemical and Biophysical Research Communications 30 August 2013, Vol.438(3), pp.479-482
Main Author: Wazir, Romel
Other Authors: Luo, De-Yi , Dai, Yi , Yue, Xuan , Tian, Ye , Wang, Kun-Jie
Format: Electronic Article Electronic Article
Language: English
Subjects:
Pkc
Jnk
P38
Pkc
Jnk
P38
ID: ISSN: 0006-291X ; E-ISSN: 1090-2104 ; DOI: 10.1016/j.bbrc.2013.07.115
Link: http://dx.doi.org/10.1016/j.bbrc.2013.07.115
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recordid: elsevier_sdoi_10_1016_j_bbrc_2013_07_115
title: Expression and proliferation profiles of PKC, JNK and p38MAPK in physiologically stretched human bladder smooth muscle cells
format: Article
creator:
  • Wazir, Romel
  • Luo, De-Yi
  • Dai, Yi
  • Yue, Xuan
  • Tian, Ye
  • Wang, Kun-Jie
subjects:
  • Bladder Smooth Muscle Cell
  • Stretch
  • Proliferation
  • Pkc
  • Jnk
  • P38
  • Bladder Smooth Muscle Cell
  • Stretch
  • Proliferation
  • Pkc
  • Jnk
  • P38
  • Biology
  • Chemistry
  • Anatomy & Physiology
ispartof: Biochemical and Biophysical Research Communications, 30 August 2013, Vol.438(3), pp.479-482
description: •Stretch induces proliferation in human bladder smooth muscle cells (HBSMC).•5% Equibiaxial elongation produces maximum proliferation.•Physiologic stretch decreases apoptotic cell death.•PKC is involved in functional modulation of bladder.•JNK and p38 are not involved in proliferating HBSMC. To determine protein kinase C (PKC), c-Jun NH2-Terminal Kinase (JNK) and P38 mitogen-activated protein kinases (p38MAPK) expression levels and effects of their respective inhibitors on proliferation of human bladder smooth muscle cells (HBSMCs) when physiologically stretched in vitro. HBSMCs were grown on silicone membrane and stretch was applied under varying conditions; (equibiaxial elongation: 2.5%, 5%, 10%, 15%, 20%, 25%), (frequency: 0.05, 0.1, 0.2, 0.5, 1Hz). Optimal physiological stretch was established by assessing proliferation with 5-Bromo-2-deoxyuridine (BrdU) assay and flow cytometry. PKC, JNK and p38 expression levels were analyzed by Western...
language: eng
source:
identifier: ISSN: 0006-291X ; E-ISSN: 1090-2104 ; DOI: 10.1016/j.bbrc.2013.07.115
fulltext: fulltext
issn:
  • 0006-291X
  • 0006291X
  • 1090-2104
  • 10902104
url: Link


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titleExpression and proliferation profiles of PKC, JNK and p38MAPK in physiologically stretched human bladder smooth muscle cells
creatorWazir, Romel ; Luo, De-Yi ; Dai, Yi ; Yue, Xuan ; Tian, Ye ; Wang, Kun-Jie
ispartofBiochemical and Biophysical Research Communications, 30 August 2013, Vol.438(3), pp.479-482
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description•Stretch induces proliferation in human bladder smooth muscle cells (HBSMC).•5% Equibiaxial elongation produces maximum proliferation.•Physiologic stretch decreases apoptotic cell death.•PKC is involved in functional modulation of bladder.•JNK and p38 are not involved in proliferating HBSMC. To determine protein kinase C (PKC), c-Jun NH2-Terminal Kinase (JNK) and P38 mitogen-activated protein kinases (p38MAPK) expression levels and effects of their respective inhibitors on proliferation of human bladder smooth muscle cells (HBSMCs) when physiologically stretched in vitro. HBSMCs were grown on silicone membrane and stretch was applied under varying conditions; (equibiaxial elongation: 2.5%, 5%, 10%, 15%, 20%, 25%), (frequency: 0.05, 0.1, 0.2, 0.5, 1Hz). Optimal physiological stretch was established by assessing proliferation with 5-Bromo-2-deoxyuridine (BrdU) assay and flow cytometry. PKC, JNK and p38 expression levels were analyzed by Western...
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titleExpression and proliferation profiles of PKC, JNK and p38MAPK in physiologically stretched human bladder smooth muscle cells
description

•Stretch induces proliferation in human bladder smooth muscle cells (HBSMC).•5% Equibiaxial elongation produces maximum proliferation.•Physiologic stretch decreases apoptotic cell death.•PKC is involved in functional modulation of bladder.•JNK and p38 are not involved in proliferating HBSMC.

To determine protein kinase C (PKC), c-Jun NH2-Terminal Kinase (JNK) and P38 mitogen-activated protein kinases (p38MAPK) expression levels and effects of their respective inhibitors on proliferation of human bladder smooth muscle cells (HBSMCs) when physiologically stretched in vitro.

HBSMCs were grown on silicone membrane and stretch was applied under varying conditions; (equibiaxial elongation: 2.5%, 5%, 10%, 15%, 20%, 25%), (frequency: 0.05, 0.1, 0.2, 0.5, 1Hz). Optimal physiological stretch was established by assessing proliferation with 5-Bromo-2-deoxyuridine (BrdU) assay and flow cytometry. PKC, JNK and p38 expression levels were analyzed by Western...

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•Stretch induces proliferation in human bladder smooth muscle cells (HBSMC).•5% Equibiaxial elongation produces maximum proliferation.•Physiologic stretch decreases apoptotic cell death.•PKC is involved in functional modulation of bladder.•JNK and p38 are not involved in proliferating HBSMC.

To determine protein kinase C (PKC), c-Jun NH2-Terminal Kinase (JNK) and P38 mitogen-activated protein kinases (p38MAPK) expression levels and effects of their respective inhibitors on proliferation of human bladder smooth muscle cells (HBSMCs) when physiologically stretched in vitro.

HBSMCs were grown on silicone membrane and stretch was applied under varying conditions; (equibiaxial elongation: 2.5%, 5%, 10%, 15%, 20%, 25%), (frequency: 0.05, 0.1, 0.2, 0.5, 1Hz). Optimal physiological stretch was established by assessing proliferation with 5-Bromo-2-deoxyuridine (BrdU) assay and flow cytometry. PKC, JNK and p38 expression levels were analyzed by Western...

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date2013-08-30