schliessen

Filtern

 

Bibliotheken

A porous photocurable elastomer for cell encapsulation and culture

Encapsulating cells within a polymer matrix creates a three-dimensional (3D) scaffold that may more accurately represent the native microenvironment and cell organization. Here we report a porous scaffold prepared from a photocurable elastomer, poly(glycerolco-sebacate)-acrylate (PGSA). The scaffold... Full description

Journal Title: Biomaterials 2007, Vol.28(32), pp.4826-4835
Main Author: Gerecht, Sharon
Other Authors: Townsend, Seth A , Pressler, Heather , Zhu, Han , Nijst, Christiaan L.E , Bruggeman, Joost P , Nichol, Jason W , Langer, Robert
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2007.07.039
Link: https://www.sciencedirect.com/science/article/pii/S0142961207005893
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: elsevier_sdoi_10_1016_j_biomaterials_2007_07_039
title: A porous photocurable elastomer for cell encapsulation and culture
format: Article
creator:
  • Gerecht, Sharon
  • Townsend, Seth A
  • Pressler, Heather
  • Zhu, Han
  • Nijst, Christiaan L.E
  • Bruggeman, Joost P
  • Nichol, Jason W
  • Langer, Robert
subjects:
  • Biocompatibility
  • Cell Encapsulation
  • Elastomer
  • Nerve
  • Scaffold
  • Stem Cell
  • Medicine
  • Engineering
ispartof: Biomaterials, 2007, Vol.28(32), pp.4826-4835
description: Encapsulating cells within a polymer matrix creates a three-dimensional (3D) scaffold that may more accurately represent the native microenvironment and cell organization. Here we report a porous scaffold prepared from a photocurable elastomer, poly(glycerolco-sebacate)-acrylate (PGSA). The scaffold porosity, swelling, mass loss, toxicity and mechanical properties, suggest that porous PGSA could be used to support the growth and differentiation of encapsulated cells. Neuroblastoma (NB) and human embryonic stem cells (hESCs) were encapsulated into the matrix and found to adhere to the material and interact with each other within 24 h. After 7 days, encapsulated NB cells were found to grow, and form matrix fibrils and tissue. Undifferentiated hESCs proliferated and differentiated in the PGSA scaffold. experiments showed that both porous scaffolds have similar biocompatibility profiles as non-porous PGSA, but porous PGSA promotes tissue...
language: eng
source:
identifier: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2007.07.039
fulltext: fulltext
issn:
  • 0142-9612
  • 01429612
  • 1878-5905
  • 18785905
url: Link


@attributes
ID11076984
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordiddoi_10_1016_j_biomaterials_2007_07_039
sourceidelsevier_s
recordidTN_elsevier_sdoi_10_1016_j_biomaterials_2007_07_039
sourcesystemPC
dbid
0--K
1--M
2.FO
3.~1
41B1
51P~
61RT
71~.
8457
94G.
107-5
118P~
129JM
139JN
14AABNK
15AAEDT
16AAEPC
17AAKOC
18AAOAW
19AAQFI
20ABFNM
21ABGSF
22ABNUV
23ABXRA
24ABYKQ
25ACDAQ
26ACIUM
27ACRLP
28ADEWK
29ADTZH
30ADUVX
31AECPX
32AEHWI
33AEKER
34AEVXI
35AEZYN
36AFKWA
37AFTJW
38AFXIZ
39AGHFR
40AGRDE
41AGUBO
42AGYEJ
43AHHHB
44AHPOS
45AIKHN
46AITUG
47AJBFU
48AJOXV
49AJUYK
50AMFUW
51BJAXD
52BLXMC
53DOVZS
54ENUVR
55EO8
56EO9
57EP2
58EP3
59FDB
60FGOYB
61FIRID
62FNPLU
63G-Q
64GBLVA
65HMK
66HMO
67J1W
68JJJVA
69KOM
70MAGPM
71OAUVE
72OB-
73OM.
74P-8
75P-9
76PC.
77Q38
78R2-
79RPZ
80SAE
81SCC
82SDF
83SDG
84SDP
85SES
86SEW
87SMS
88SPC
89SSG
90SSM
91SST
92SSU
93SSZ
94T5K
95Z5R
96~G-
pqid20330224
galeid168095856
display
typearticle
titleA porous photocurable elastomer for cell encapsulation and culture
creatorGerecht, Sharon ; Townsend, Seth A ; Pressler, Heather ; Zhu, Han ; Nijst, Christiaan L.E ; Bruggeman, Joost P ; Nichol, Jason W ; Langer, Robert
ispartofBiomaterials, 2007, Vol.28(32), pp.4826-4835
identifier
subjectBiocompatibility ; Cell Encapsulation ; Elastomer ; Nerve ; Scaffold ; Stem Cell ; Medicine ; Engineering
descriptionEncapsulating cells within a polymer matrix creates a three-dimensional (3D) scaffold that may more accurately represent the native microenvironment and cell organization. Here we report a porous scaffold prepared from a photocurable elastomer, poly(glycerolco-sebacate)-acrylate (PGSA). The scaffold porosity, swelling, mass loss, toxicity and mechanical properties, suggest that porous PGSA could be used to support the growth and differentiation of encapsulated cells. Neuroblastoma (NB) and human embryonic stem cells (hESCs) were encapsulated into the matrix and found to adhere to the material and interact with each other within 24 h. After 7 days, encapsulated NB cells were found to grow, and form matrix fibrils and tissue. Undifferentiated hESCs proliferated and differentiated in the PGSA scaffold. experiments showed that both porous scaffolds have similar biocompatibility profiles as non-porous PGSA, but porous PGSA promotes tissue...
languageeng
source
version7
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961207005893$$EView_record_in_ScienceDirect_(Access_to_full_text_may_be_restricted)
search
creatorcontrib
0Gerecht, Sharon
1Townsend, Seth A
2Pressler, Heather
3Zhu, Han
4Nijst, Christiaan L.E
5Bruggeman, Joost P
6Nichol, Jason W
7Langer, Robert
titleA porous photocurable elastomer for cell encapsulation and culture
description

Encapsulating cells within a polymer matrix creates a three-dimensional (3D) scaffold that may more accurately represent the native microenvironment and cell organization. Here we report a porous scaffold prepared from a photocurable elastomer, poly(glycerolco-sebacate)-acrylate (PGSA). The scaffold porosity, swelling, mass loss, toxicity and mechanical properties, suggest that porous PGSA could be used to support the growth and differentiation of encapsulated cells. Neuroblastoma (NB) and human embryonic stem cells (hESCs) were encapsulated into the matrix and found to adhere to the material and interact with each other within 24

h. After 7 days, encapsulated NB cells were found to grow, and form matrix fibrils and tissue. Undifferentiated hESCs proliferated and differentiated in the PGSA scaffold.

experiments showed that both porous scaffolds have similar biocompatibility profiles as non-porous PGSA, but porous PGSA promotes tissue...

subject
0Biocompatibility
1Cell Encapsulation
2Elastomer
3Nerve
4Scaffold
5Stem Cell
6Medicine
7Engineering
general
0English
1Elsevier Ltd
210.1016/j.biomaterials.2007.07.039
3ScienceDirect (Elsevier)
4ScienceDirect Journals (Elsevier)
sourceidelsevier_s
recordidelsevier_sdoi_10_1016_j_biomaterials_2007_07_039
issn
00142-9612
101429612
21878-5905
318785905
rsrctypearticle
creationdate2007
addtitleBiomaterials
searchscope
0elsevier_full
1elsevier2
scope
0elsevier_full
1elsevier2
lsr44$$EView_record_in_ScienceDirect_(Access_to_full_text_may_be_restricted)
tmp01ScienceDirect Journals (Elsevier)
tmp02
0--K
1--M
2.FO
3.~1
41B1
51P~
61RT
71~.
8457
94G.
107-5
118P~
129JM
139JN
14AABNK
15AAEDT
16AAEPC
17AAKOC
18AAOAW
19AAQFI
20ABFNM
21ABGSF
22ABNUV
23ABXRA
24ABYKQ
25ACDAQ
26ACIUM
27ACRLP
28ADEWK
29ADTZH
30ADUVX
31AECPX
32AEHWI
33AEKER
34AEVXI
35AEZYN
36AFKWA
37AFTJW
38AFXIZ
39AGHFR
40AGRDE
41AGUBO
42AGYEJ
43AHHHB
44AHPOS
45AIKHN
46AITUG
47AJBFU
48AJOXV
49AJUYK
50AMFUW
51BJAXD
52BLXMC
53DOVZS
54ENUVR
55EO8
56EO9
57EP2
58EP3
59FDB
60FGOYB
61FIRID
62FNPLU
63G-Q
64GBLVA
65HMK
66HMO
67J1W
68JJJVA
69KOM
70MAGPM
71OAUVE
72OB-
73OM.
74P-8
75P-9
76PC.
77Q38
78R2-
79RPZ
80SAE
81SCC
82SDF
83SDG
84SDP
85SES
86SEW
87SMS
88SPC
89SSG
90SSM
91SST
92SSU
93SSZ
94T5K
95Z5R
96~G-
startdate20070101
enddate20071231
lsr40Biomaterials, 2007, Vol.28 (32), pp.4826-4835
doi10.1016/j.biomaterials.2007.07.039
citationpf 4826 pt 4835 vol 28 issue 32
lsr30VSR-Enriched:[galeid, pqid]
sort
titleA porous photocurable elastomer for cell encapsulation and culture
authorGerecht, Sharon ; Townsend, Seth A ; Pressler, Heather ; Zhu, Han ; Nijst, Christiaan L.E ; Bruggeman, Joost P ; Nichol, Jason W ; Langer, Robert
creationdate20070000
lso0120070000
facets
frbrgroupid7491510597181507312
frbrtype5
newrecords20190904
languageeng
topic
0Biocompatibility
1Cell Encapsulation
2Elastomer
3Nerve
4Scaffold
5Stem Cell
6Medicine
7Engineering
collectionScienceDirect (Elsevier)
prefilterarticles
rsrctypearticles
creatorcontrib
0Gerecht, Sharon
1Townsend, Seth A
2Pressler, Heather
3Zhu, Han
4Nijst, Christiaan L.E
5Bruggeman, Joost P
6Nichol, Jason W
7Langer, Robert
jtitleBiomaterials
creationdate2007
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Gerecht
1Townsend
2Pressler
3Zhu
4Nijst
5Bruggeman
6Nichol
7Langer
aufirst
0Sharon
1Seth A
2Heather
3Han
4Christiaan L.E
5Joost P
6Jason W
7Robert
auinitS
auinit1S
au
0Gerecht, Sharon
1Townsend, Seth A
2Pressler, Heather
3Zhu, Han
4Nijst, Christiaan L.E
5Bruggeman, Joost P
6Nichol, Jason W
7Langer, Robert
atitleA porous photocurable elastomer for cell encapsulation and culture
jtitleBiomaterials
date2007
risdate2007
volume28
issue32
spage4826
epage4835
pages4826-4835
issn0142-9612
eissn1878-5905
formatjournal
genrearticle
ristypeJOUR
abstract

Encapsulating cells within a polymer matrix creates a three-dimensional (3D) scaffold that may more accurately represent the native microenvironment and cell organization. Here we report a porous scaffold prepared from a photocurable elastomer, poly(glycerolco-sebacate)-acrylate (PGSA). The scaffold porosity, swelling, mass loss, toxicity and mechanical properties, suggest that porous PGSA could be used to support the growth and differentiation of encapsulated cells. Neuroblastoma (NB) and human embryonic stem cells (hESCs) were encapsulated into the matrix and found to adhere to the material and interact with each other within 24

h. After 7 days, encapsulated NB cells were found to grow, and form matrix fibrils and tissue. Undifferentiated hESCs proliferated and differentiated in the PGSA scaffold.

experiments showed that both porous scaffolds have similar biocompatibility profiles as non-porous PGSA, but porous PGSA promotes tissue...

pubElsevier Ltd
doi10.1016/j.biomaterials.2007.07.039
lad01Biomaterials