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The effect of sustained delivery of vascular endothelial growth factor on angiogenesis in tissue-engineered intestine

Our group has previously created a functional neointestine that is capable of restoring absorptive function. However, the endogenous level of vascular endothelial growth factor (VEGF) is markedly reduced in the construct compared to native bowel. Therefore, we wanted to locally deliver VEGF in a sus... Full description

Journal Title: Biomaterials 2008, Vol.29(19), pp.2884-2890
Main Author: Rocha, Flavio G
Other Authors: Sundback, Cathryn A , Krebs, Nicholas J , Leach, J. Kent , Mooney, David J , Ashley, Stanley W , Vacanti, Joseph P , Whang, Edward E
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2008.03.026
Link: https://www.sciencedirect.com/science/article/pii/S014296120800197X
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recordid: elsevier_sdoi_10_1016_j_biomaterials_2008_03_026
title: The effect of sustained delivery of vascular endothelial growth factor on angiogenesis in tissue-engineered intestine
format: Article
creator:
  • Rocha, Flavio G
  • Sundback, Cathryn A
  • Krebs, Nicholas J
  • Leach, J. Kent
  • Mooney, David J
  • Ashley, Stanley W
  • Vacanti, Joseph P
  • Whang, Edward E
subjects:
  • Small Intestine
  • Vegf
  • Angiogenesis
  • Microspheres
  • Plga
  • Medicine
  • Engineering
ispartof: Biomaterials, 2008, Vol.29(19), pp.2884-2890
description: Our group has previously created a functional neointestine that is capable of restoring absorptive function. However, the endogenous level of vascular endothelial growth factor (VEGF) is markedly reduced in the construct compared to native bowel. Therefore, we wanted to locally deliver VEGF in a sustained fashion to upregulate angiogenesis in the neointestine. Rat recombinant VEGF was encapsulated in poly(lactide- -glycolide) microspheres by a double emulsion method. Release kinetics and bioactivity were determined in vitro. Tissue-engineered intestine was generated by seeding donor neonatal rat intestinal organoid units onto a biodegradable polyglycolic acid scaffold along with VEGF-containing or empty microspheres, and wrapped in the omentum of recipient rats. After 4 weeks, the neointestinal cysts were analyzed for morphometry, VEGF levels, epithelial proliferation, and capillary density. Sustained release of biologically active VEGF was confirmed by...
language: eng
source:
identifier: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2008.03.026
fulltext: fulltext
issn:
  • 0142-9612
  • 01429612
  • 1878-5905
  • 18785905
url: Link


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titleThe effect of sustained delivery of vascular endothelial growth factor on angiogenesis in tissue-engineered intestine
creatorRocha, Flavio G ; Sundback, Cathryn A ; Krebs, Nicholas J ; Leach, J. Kent ; Mooney, David J ; Ashley, Stanley W ; Vacanti, Joseph P ; Whang, Edward E
ispartofBiomaterials, 2008, Vol.29(19), pp.2884-2890
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subjectSmall Intestine ; Vegf ; Angiogenesis ; Microspheres ; Plga ; Medicine ; Engineering
descriptionOur group has previously created a functional neointestine that is capable of restoring absorptive function. However, the endogenous level of vascular endothelial growth factor (VEGF) is markedly reduced in the construct compared to native bowel. Therefore, we wanted to locally deliver VEGF in a sustained fashion to upregulate angiogenesis in the neointestine. Rat recombinant VEGF was encapsulated in poly(lactide- -glycolide) microspheres by a double emulsion method. Release kinetics and bioactivity were determined in vitro. Tissue-engineered intestine was generated by seeding donor neonatal rat intestinal organoid units onto a biodegradable polyglycolic acid scaffold along with VEGF-containing or empty microspheres, and wrapped in the omentum of recipient rats. After 4 weeks, the neointestinal cysts were analyzed for morphometry, VEGF levels, epithelial proliferation, and capillary density. Sustained release of biologically active VEGF was confirmed by...
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titleThe effect of sustained delivery of vascular endothelial growth factor on angiogenesis in tissue-engineered intestine
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Our group has previously created a functional neointestine that is capable of restoring absorptive function. However, the endogenous level of vascular endothelial growth factor (VEGF) is markedly reduced in the construct compared to native bowel. Therefore, we wanted to locally deliver VEGF in a sustained fashion to upregulate angiogenesis in the neointestine. Rat recombinant VEGF was encapsulated in poly(lactide-

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abstract

Our group has previously created a functional neointestine that is capable of restoring absorptive function. However, the endogenous level of vascular endothelial growth factor (VEGF) is markedly reduced in the construct compared to native bowel. Therefore, we wanted to locally deliver VEGF in a sustained fashion to upregulate angiogenesis in the neointestine. Rat recombinant VEGF was encapsulated in poly(lactide-

-glycolide) microspheres by a double emulsion method. Release kinetics and bioactivity were determined in vitro. Tissue-engineered intestine was generated by seeding donor neonatal rat intestinal organoid units onto a biodegradable polyglycolic acid scaffold along with VEGF-containing or empty microspheres, and wrapped in the omentum of recipient rats. After 4 weeks, the neointestinal cysts were analyzed for morphometry, VEGF levels, epithelial proliferation, and capillary density. Sustained release of biologically active VEGF was confirmed by...

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doi10.1016/j.biomaterials.2008.03.026
lad01Biomaterials