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Intervertebral disc regeneration in an ex vivo culture system using mesenchymal stem cells and platelet-rich plasma

An degenerative intervertebral disc (IVD) organ culture system was established for the screening of disc regeneration agents. Its application was demonstrated by a stem cell and growth factor-based therapeutic approach for the amelioration of IVD. An culture system using chymopapain to partially dig... Full description

Journal Title: Biomaterials 2009, Vol.30(29), pp.5523-5533
Main Author: Chen, Wei-Hong
Other Authors: Liu, Hen-Yu , Lo, Wen-Cheng , Wu, Shinn-Chih , Chi, Chau-Hwa , Chang, Hsueh-Yuan , Hsiao, Shih-Hsiang , Wu, Chih-Hsiung , Chiu, Wen-Ta , Chen, Bao-Ji , Deng, Win-Ping
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2009.07.019
Link: https://www.sciencedirect.com/science/article/pii/S0142961209007169
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recordid: elsevier_sdoi_10_1016_j_biomaterials_2009_07_019
title: Intervertebral disc regeneration in an ex vivo culture system using mesenchymal stem cells and platelet-rich plasma
format: Article
creator:
  • Chen, Wei-Hong
  • Liu, Hen-Yu
  • Lo, Wen-Cheng
  • Wu, Shinn-Chih
  • Chi, Chau-Hwa
  • Chang, Hsueh-Yuan
  • Hsiao, Shih-Hsiang
  • Wu, Chih-Hsiung
  • Chiu, Wen-Ta
  • Chen, Bao-Ji
  • Deng, Win-Ping
subjects:
  • Intervertebral Disc
  • Mesenchymal Stem Cell
  • Platelet-Rich Plasma
  • Nucleus Pulposus
  • Regeneration
  • Medicine
  • Engineering
ispartof: Biomaterials, 2009, Vol.30(29), pp.5523-5533
description: An degenerative intervertebral disc (IVD) organ culture system was established for the screening of disc regeneration agents. Its application was demonstrated by a stem cell and growth factor-based therapeutic approach for the amelioration of IVD. An culture system using chymopapain to partially digest nucleus proposus tissue was established to mimic human IVD degeneration. This system was then used for the evaluation of different therapeutic regimens including: mesenchymal stem cell derived from eGFP-transgenic porcine (MSC-GFP), platelet-rich plasma (PRP) and MSC-GFP/PRP combined treatment, and confirmed in animal model. Chondrogenic-specific gene products including and were found upregulated and chondrogenic matrix deposition increased, as evident by sustained fluorescent signals over 4 weeks, in the MSC-GFP implanted group. Previously, we demonstrated...
language: eng
source:
identifier: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2009.07.019
fulltext: fulltext
issn:
  • 0142-9612
  • 01429612
  • 1878-5905
  • 18785905
url: Link


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titleIntervertebral disc regeneration in an ex vivo culture system using mesenchymal stem cells and platelet-rich plasma
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subjectIntervertebral Disc ; Mesenchymal Stem Cell ; Platelet-Rich Plasma ; Nucleus Pulposus ; Regeneration ; Medicine ; Engineering
descriptionAn degenerative intervertebral disc (IVD) organ culture system was established for the screening of disc regeneration agents. Its application was demonstrated by a stem cell and growth factor-based therapeutic approach for the amelioration of IVD. An culture system using chymopapain to partially digest nucleus proposus tissue was established to mimic human IVD degeneration. This system was then used for the evaluation of different therapeutic regimens including: mesenchymal stem cell derived from eGFP-transgenic porcine (MSC-GFP), platelet-rich plasma (PRP) and MSC-GFP/PRP combined treatment, and confirmed in animal model. Chondrogenic-specific gene products including and were found upregulated and chondrogenic matrix deposition increased, as evident by sustained fluorescent signals over 4 weeks, in the MSC-GFP implanted group. Previously, we demonstrated...
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