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The use of injectable, thermosensitive poly(organophosphazene)–RGD conjugates for the enhancement of mesenchymal stem cell osteogenic differentiation

An injectable and thermosensitive poly(organophosphazene)–RGD conjugate to enhance functionality was synthesized by a covalent amide linkage between a cell adhesion peptide, GRGDS and carboxylic acid-terminated poly(organophosphazene). The aqueous solutions of synthesized poly(organophosphazene)–GRG... Full description

Journal Title: Biomaterials 2009, Vol.30(31), pp.6295-6308
Main Author: Chun, Changju
Other Authors: Lim, Hye Jin , Hong, Ki-Yun , Park, Keun-Hong , Song, Soo-Chang
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2009.08.011
Link: http://dx.doi.org/10.1016/j.biomaterials.2009.08.011
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recordid: elsevier_sdoi_10_1016_j_biomaterials_2009_08_011
title: The use of injectable, thermosensitive poly(organophosphazene)–RGD conjugates for the enhancement of mesenchymal stem cell osteogenic differentiation
format: Article
creator:
  • Chun, Changju
  • Lim, Hye Jin
  • Hong, Ki-Yun
  • Park, Keun-Hong
  • Song, Soo-Chang
subjects:
  • Thermosensitive
  • Injectable
  • Polyphosphazene–Rgd Conjugate
  • Mesenchymal Stem Cells (Mscs)
  • Osteogenic Differentiation
  • Hydrogel
  • Thermosensitive
  • Injectable
  • Polyphosphazene–Rgd Conjugate
  • Mesenchymal Stem Cells (Mscs)
  • Osteogenic Differentiation
  • Hydrogel
  • Medicine
  • Engineering
ispartof: Biomaterials, 2009, Vol.30(31), pp.6295-6308
description: An injectable and thermosensitive poly(organophosphazene)–RGD conjugate to enhance functionality was synthesized by a covalent amide linkage between a cell adhesion peptide, GRGDS and carboxylic acid-terminated poly(organophosphazene). The aqueous solutions of synthesized poly(organophosphazene)–GRGDS conjugates existed in an injectable fluid state at room temperature and immediately formed a hydrogel at body temperature. The rabbit mesenchymal stem cells (rMSCs) on the polymer–GRGDS conjugate (conjugate 1- 2, 0.05 mol fraction as GRGDS) hydrogel constructs using an injection method into a nude mouse were proved to express markers at mRNA level for all stages towards osteogenesis and mainly a sharp increase of osteocalcin (OCN, a typical late osteogenic differentiation marker) levels at 4th week post-induction indicated that the maturation process has started within this period. By histological and immunohistochemical evaluations, significantly high mineralization level...
language: eng
source:
identifier: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2009.08.011
fulltext: fulltext
issn:
  • 0142-9612
  • 01429612
  • 1878-5905
  • 18785905
url: Link


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titleThe use of injectable, thermosensitive poly(organophosphazene)–RGD conjugates for the enhancement of mesenchymal stem cell osteogenic differentiation
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subjectThermosensitive ; Injectable ; Polyphosphazene–Rgd Conjugate ; Mesenchymal Stem Cells (Mscs) ; Osteogenic Differentiation ; Hydrogel ; Thermosensitive ; Injectable ; Polyphosphazene–Rgd Conjugate ; Mesenchymal Stem Cells (Mscs) ; Osteogenic Differentiation ; Hydrogel ; Medicine ; Engineering
descriptionAn injectable and thermosensitive poly(organophosphazene)–RGD conjugate to enhance functionality was synthesized by a covalent amide linkage between a cell adhesion peptide, GRGDS and carboxylic acid-terminated poly(organophosphazene). The aqueous solutions of synthesized poly(organophosphazene)–GRGDS conjugates existed in an injectable fluid state at room temperature and immediately formed a hydrogel at body temperature. The rabbit mesenchymal stem cells (rMSCs) on the polymer–GRGDS conjugate (conjugate 1- 2, 0.05 mol fraction as GRGDS) hydrogel constructs using an injection method into a nude mouse were proved to express markers at mRNA level for all stages towards osteogenesis and mainly a sharp increase of osteocalcin (OCN, a typical late osteogenic differentiation marker) levels at 4th week post-induction indicated that the maturation process has started within this period. By histological and immunohistochemical evaluations, significantly high mineralization level...
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An injectable and thermosensitive poly(organophosphazene)–RGD conjugate to enhance functionality was synthesized by a covalent amide linkage between a cell adhesion peptide, GRGDS and carboxylic acid-terminated poly(organophosphazene). The aqueous solutions of synthesized poly(organophosphazene)–GRGDS conjugates existed in an injectable fluid state at room temperature and immediately formed a hydrogel at body temperature. The rabbit mesenchymal stem cells (rMSCs) on the polymer–GRGDS conjugate (conjugate 1- 2, 0.05 mol fraction as GRGDS) hydrogel constructs using an injection method into a nude mouse were proved to express markers at mRNA level for all stages towards osteogenesis and mainly a sharp increase of osteocalcin (OCN, a typical late osteogenic differentiation marker) levels at 4th week post-induction indicated that the maturation process has started within this period. By histological and immunohistochemical evaluations, significantly high mineralization level...

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abstract

An injectable and thermosensitive poly(organophosphazene)–RGD conjugate to enhance functionality was synthesized by a covalent amide linkage between a cell adhesion peptide, GRGDS and carboxylic acid-terminated poly(organophosphazene). The aqueous solutions of synthesized poly(organophosphazene)–GRGDS conjugates existed in an injectable fluid state at room temperature and immediately formed a hydrogel at body temperature. The rabbit mesenchymal stem cells (rMSCs) on the polymer–GRGDS conjugate (conjugate 1- 2, 0.05 mol fraction as GRGDS) hydrogel constructs using an injection method into a nude mouse were proved to express markers at mRNA level for all stages towards osteogenesis and mainly a sharp increase of osteocalcin (OCN, a typical late osteogenic differentiation marker) levels at 4th week post-induction indicated that the maturation process has started within this period. By histological and immunohistochemical evaluations, significantly high mineralization level...

pubElsevier Ltd
doi10.1016/j.biomaterials.2009.08.011
lad01Biomaterials