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Characterization of valvular interstitial cell function in three dimensional matrix metalloproteinase degradable PEG hydrogels

Valvular interstitial cells (VICs) maintain functional heart valve structure and display transient fibroblast and myofibroblast properties. Most cell characterization studies have been performed on plastic dishes; while insightful, these systems are limited. Thus, a matrix metalloproteinase (MMP) de... Full description

Journal Title: Biomaterials 2009, Vol.30(34), pp.6593-6603
Main Author: Benton, Julie A
Other Authors: Fairbanks, Benjamin D , Anseth, Kristi S
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2009.08.031
Link: https://www.sciencedirect.com/science/article/pii/S0142961209008746
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recordid: elsevier_sdoi_10_1016_j_biomaterials_2009_08_031
title: Characterization of valvular interstitial cell function in three dimensional matrix metalloproteinase degradable PEG hydrogels
format: Article
creator:
  • Benton, Julie A
  • Fairbanks, Benjamin D
  • Anseth, Kristi S
subjects:
  • Fibroblast
  • Heart Valve
  • Hydrogel
  • Peptide
  • Photopolymerisation
  • Matrix Metalloproteinase
  • Medicine
  • Engineering
ispartof: Biomaterials, 2009, Vol.30(34), pp.6593-6603
description: Valvular interstitial cells (VICs) maintain functional heart valve structure and display transient fibroblast and myofibroblast properties. Most cell characterization studies have been performed on plastic dishes; while insightful, these systems are limited. Thus, a matrix metalloproteinase (MMP) degradable poly(ethylene glycol) (PEG) hydrogel system is proposed in this communication as a useful tool for characterizing VIC function in 3D. When encapsulated, VICs attained spread morphology, and proliferated and migrated as shown through real-time cell microscopy. Additionally, fibronectin derived pendant RGD was incorporated into the system to promote integrin binding. As RGD concentration increased from 0 to 2000 μ , VIC process extension and integrin α β binding increased within two days. By day 10, integrin binding was equalized between conditions. VIC morphology and rate of process...
language: eng
source:
identifier: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2009.08.031
fulltext: fulltext
issn:
  • 0142-9612
  • 01429612
  • 1878-5905
  • 18785905
url: Link


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titleCharacterization of valvular interstitial cell function in three dimensional matrix metalloproteinase degradable PEG hydrogels
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subjectFibroblast ; Heart Valve ; Hydrogel ; Peptide ; Photopolymerisation ; Matrix Metalloproteinase ; Medicine ; Engineering
descriptionValvular interstitial cells (VICs) maintain functional heart valve structure and display transient fibroblast and myofibroblast properties. Most cell characterization studies have been performed on plastic dishes; while insightful, these systems are limited. Thus, a matrix metalloproteinase (MMP) degradable poly(ethylene glycol) (PEG) hydrogel system is proposed in this communication as a useful tool for characterizing VIC function in 3D. When encapsulated, VICs attained spread morphology, and proliferated and migrated as shown through real-time cell microscopy. Additionally, fibronectin derived pendant RGD was incorporated into the system to promote integrin binding. As RGD concentration increased from 0 to 2000 μ , VIC process extension and integrin α β binding increased within two days. By day 10, integrin binding was equalized between conditions. VIC morphology and rate of process...
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