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Controlled architectural and chemotactic studies of 3D cell migration

Chemotaxis plays a critical role in tissue development and wound repair, and is widely studied using model systems in applications such as immunotherapy. However, typical chemotactic models employ 2D systems that are less physiologically relevant or use end-point assays, that reveal little about the... Full description

Journal Title: Biomaterials 2011, Vol.32(10), pp.2634-2641
Main Author: Tayalia, Prakriti
Other Authors: Mazur, Eric , Mooney, David J
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2010.12.019
Link: https://www.sciencedirect.com/science/article/pii/S0142961210015796
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recordid: elsevier_sdoi_10_1016_j_biomaterials_2010_12_019
title: Controlled architectural and chemotactic studies of 3D cell migration
format: Article
creator:
  • Tayalia, Prakriti
  • Mazur, Eric
  • Mooney, David J
subjects:
  • Live Imaging
  • Dendritic Cells
  • Two-Photon Polymerization
  • Microfabrication
  • Scaffolds
  • Ccl19 Gradient
  • Medicine
  • Engineering
ispartof: Biomaterials, 2011, Vol.32(10), pp.2634-2641
description: Chemotaxis plays a critical role in tissue development and wound repair, and is widely studied using model systems in applications such as immunotherapy. However, typical chemotactic models employ 2D systems that are less physiologically relevant or use end-point assays, that reveal little about the stepwise dynamics of the migration process. To overcome these limitations, we developed a new model system using microfabrication techniques, sustained drug delivery approaches, and theoretical modeling of chemotactic agent diffusion. This model system allows us to study the effects of 3D architecture and chemotactic agent gradient on immune cell migration in real time. We find that dendritic cell migration is characterized by a strong interplay between matrix architecture and chemotactic gradients, and migration is also influenced dramatically by the cell activation state. Our results indicate that Lipopolysaccharide-activated dendritic cells studied in a...
language: eng
source:
identifier: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2010.12.019
fulltext: fulltext
issn:
  • 0142-9612
  • 01429612
  • 1878-5905
  • 18785905
url: Link


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subjectLive Imaging ; Dendritic Cells ; Two-Photon Polymerization ; Microfabrication ; Scaffolds ; Ccl19 Gradient ; Medicine ; Engineering
descriptionChemotaxis plays a critical role in tissue development and wound repair, and is widely studied using model systems in applications such as immunotherapy. However, typical chemotactic models employ 2D systems that are less physiologically relevant or use end-point assays, that reveal little about the stepwise dynamics of the migration process. To overcome these limitations, we developed a new model system using microfabrication techniques, sustained drug delivery approaches, and theoretical modeling of chemotactic agent diffusion. This model system allows us to study the effects of 3D architecture and chemotactic agent gradient on immune cell migration in real time. We find that dendritic cell migration is characterized by a strong interplay between matrix architecture and chemotactic gradients, and migration is also influenced dramatically by the cell activation state. Our results indicate that Lipopolysaccharide-activated dendritic cells studied in a...
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Chemotaxis plays a critical role in tissue development and wound repair, and is widely studied using

model systems in applications such as immunotherapy. However, typical chemotactic models employ 2D systems that are less physiologically relevant or use end-point assays, that reveal little about the stepwise dynamics of the migration process. To overcome these limitations, we developed a new model system using microfabrication techniques, sustained drug delivery approaches, and theoretical modeling of chemotactic agent diffusion. This model system allows us to study the effects of 3D architecture and chemotactic agent gradient on immune cell migration in real time. We find that dendritic cell migration is characterized by a strong interplay between matrix architecture and chemotactic gradients, and migration is also influenced dramatically by the cell activation state. Our results indicate that Lipopolysaccharide-activated dendritic cells studied in a...

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