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Multifunctional receptor-targeted nanocomplexes for magnetic resonance imaging and transfection of tumours

The efficient targeted delivery of nucleic acids in vivo provides some of the greatest challenges to the development of genetic therapies. We aim to develop nanocomplex formulations that achieve targeted transfection of neuroblastoma tumours that can be monitored simultaneously by MRI. Here, we have... Full description

Journal Title: Biomaterials October 2012, Vol.33(29), pp.7241-7250
Main Author: Kenny, Gavin D
Other Authors: Villegas-Llerena, Claudio , Tagalakis, Aristides D , Campbell, Frederick , Welser, Katharina , Botta, Mauro , Tabor, Alethea B , Hailes, Helen C , Lythgoe, Mark F , Hart, Stephen L
Format: Electronic Article Electronic Article
Language: English
Subjects:
Mri
Mri
ID: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2012.06.042
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recordid: elsevier_sdoi_10_1016_j_biomaterials_2012_06_042
title: Multifunctional receptor-targeted nanocomplexes for magnetic resonance imaging and transfection of tumours
format: Article
creator:
  • Kenny, Gavin D
  • Villegas-Llerena, Claudio
  • Tagalakis, Aristides D
  • Campbell, Frederick
  • Welser, Katharina
  • Botta, Mauro
  • Tabor, Alethea B
  • Hailes, Helen C
  • Lythgoe, Mark F
  • Hart, Stephen L
subjects:
  • Gene Therapy
  • Liposome
  • Mri
  • Nanocomplex
  • Peptide
  • Gene Therapy
  • Liposome
  • Mri
  • Nanocomplex
  • Peptide
  • Medicine
  • Engineering
ispartof: Biomaterials, October 2012, Vol.33(29), pp.7241-7250
description: The efficient targeted delivery of nucleic acids in vivo provides some of the greatest challenges to the development of genetic therapies. We aim to develop nanocomplex formulations that achieve targeted transfection of neuroblastoma tumours that can be monitored simultaneously by MRI. Here, we have compared nanocomplexes comprising self-assembling mixtures of liposomes, plasmid DNA and one of three different peptide ligands derived from ApoE, neurotensin and tetanus toxin for targeted transfection in vitro and in vivo. Neurotensin-targeted nanocomplexes produced the highest levels of transfection and showed a 4.7-fold increase in transfected luciferase expression over non-targeted nanocomplexes in Neuro-2A cells. Transfection of subcutaneous Neuro-2A tumours in vivo with neurotensin-targeted nanocomplexes produced a 9.3-fold increase in gene expression over non-targeted controls. Confocal microscopy analysis elucidated the time course of DNA delivery with fluorescently labelled...
language: eng
source:
identifier: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2012.06.042
fulltext: fulltext
issn:
  • 0142-9612
  • 01429612
  • 1878-5905
  • 18785905
url: Link


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titleMultifunctional receptor-targeted nanocomplexes for magnetic resonance imaging and transfection of tumours
creatorKenny, Gavin D ; Villegas-Llerena, Claudio ; Tagalakis, Aristides D ; Campbell, Frederick ; Welser, Katharina ; Botta, Mauro ; Tabor, Alethea B ; Hailes, Helen C ; Lythgoe, Mark F ; Hart, Stephen L
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subjectGene Therapy ; Liposome ; Mri ; Nanocomplex ; Peptide ; Gene Therapy ; Liposome ; Mri ; Nanocomplex ; Peptide ; Medicine ; Engineering
descriptionThe efficient targeted delivery of nucleic acids in vivo provides some of the greatest challenges to the development of genetic therapies. We aim to develop nanocomplex formulations that achieve targeted transfection of neuroblastoma tumours that can be monitored simultaneously by MRI. Here, we have compared nanocomplexes comprising self-assembling mixtures of liposomes, plasmid DNA and one of three different peptide ligands derived from ApoE, neurotensin and tetanus toxin for targeted transfection in vitro and in vivo. Neurotensin-targeted nanocomplexes produced the highest levels of transfection and showed a 4.7-fold increase in transfected luciferase expression over non-targeted nanocomplexes in Neuro-2A cells. Transfection of subcutaneous Neuro-2A tumours in vivo with neurotensin-targeted nanocomplexes produced a 9.3-fold increase in gene expression over non-targeted controls. Confocal microscopy analysis elucidated the time course of DNA delivery with fluorescently labelled...
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The efficient targeted delivery of nucleic acids in vivo provides some of the greatest challenges to the development of genetic therapies. We aim to develop nanocomplex formulations that achieve targeted transfection of neuroblastoma tumours that can be monitored simultaneously by MRI. Here, we have compared nanocomplexes comprising self-assembling mixtures of liposomes, plasmid DNA and one of three different peptide ligands derived from ApoE, neurotensin and tetanus toxin for targeted transfection in vitro and in vivo. Neurotensin-targeted nanocomplexes produced the highest levels of transfection and showed a 4.7-fold increase in transfected luciferase expression over non-targeted nanocomplexes in Neuro-2A cells. Transfection of subcutaneous Neuro-2A tumours in vivo with neurotensin-targeted nanocomplexes produced a 9.3-fold increase in gene expression over non-targeted controls. Confocal microscopy analysis elucidated the time course of DNA delivery with fluorescently labelled...

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The efficient targeted delivery of nucleic acids in vivo provides some of the greatest challenges to the development of genetic therapies. We aim to develop nanocomplex formulations that achieve targeted transfection of neuroblastoma tumours that can be monitored simultaneously by MRI. Here, we have compared nanocomplexes comprising self-assembling mixtures of liposomes, plasmid DNA and one of three different peptide ligands derived from ApoE, neurotensin and tetanus toxin for targeted transfection in vitro and in vivo. Neurotensin-targeted nanocomplexes produced the highest levels of transfection and showed a 4.7-fold increase in transfected luciferase expression over non-targeted nanocomplexes in Neuro-2A cells. Transfection of subcutaneous Neuro-2A tumours in vivo with neurotensin-targeted nanocomplexes produced a 9.3-fold increase in gene expression over non-targeted controls. Confocal microscopy analysis elucidated the time course of DNA delivery with fluorescently labelled...

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