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The cellular uptake and localization of non-emissive iridium(III) complexes as cellular reaction-based luminescence probes

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.biomaterials.2012.09.014 Byline: Chunyan Li, Yi Liu, Yongquan Wu, Yun Sun, Fuyou Li Abstract: Improvement of cellular uptake and subcellular resolution remains a major obstacle in the successful and broad appl... Full description

Journal Title: Biomaterials January 2013, Vol.34(4), pp.1223-1234
Main Author: Li, Chunyan
Other Authors: Liu, Yi , Wu, Yongquan , Sun, Yun , Li, Fuyou
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2012.09.014
Link: https://www.sciencedirect.com/science/article/pii/S0142961212010071
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recordid: elsevier_sdoi_10_1016_j_biomaterials_2012_09_014
title: The cellular uptake and localization of non-emissive iridium(III) complexes as cellular reaction-based luminescence probes
format: Article
creator:
  • Li, Chunyan
  • Liu, Yi
  • Wu, Yongquan
  • Sun, Yun
  • Li, Fuyou
subjects:
  • Iridium(III) Complex
  • Nucleus Stain
  • Fluorescent Bioimaging
  • Structure–Activity Relationship
  • Medicine
  • Engineering
ispartof: Biomaterials, January 2013, Vol.34(4), pp.1223-1234
description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.biomaterials.2012.09.014 Byline: Chunyan Li, Yi Liu, Yongquan Wu, Yun Sun, Fuyou Li Abstract: Improvement of cellular uptake and subcellular resolution remains a major obstacle in the successful and broad application of cellular optical probes. In this context, we design and synthesize seven non-emissive cyclometalated iridium(III) solvent complexes [Ir(CEN).sub.2(solv).sub.2].sup.+L.sup.- (LIr2-LIr8, in which CEN = 2-phenylpyridine (ppy) or its derivative; solv = DMSO, H.sub.2O or CH.sub.3CN; L.sup.- = PF.sub.6.sup.- or OTf.sup.-) applicable in live cell imaging to facilitate selective visualization of cellular structures. Based on the above variations (including different counter ions, solvent ligands, and CEN ligands), structure-activity relationship analyses reveal a number of clear correlations: (1) variations in counter anions and solvent ligands of iridium(III) complexes do not affect cellular imaging behavior, and (2) length of the side carbon chain in C.sup.EN ligands has significant effects on cellular uptake and localization/accumulation of iridium complexes in living cells. Moreover, investigation of the uptake mechanism via low-temperature and metabolism inhibitor assays reveal that [Ir(4-Meppy).sub.2(CH.sub.3CN).sub.2].sup.+OTf.sup.- (LIr5) with 2-phenylpyridine derivative with side-chain of methyl group at the 4-position as CEN ligand permeates the outer and nuclear membranes of living cells through an energy-dependent, non-endocytic entry pathway, and translocation of the complex from the cell periphery towards the perinuclear region possibly occurs through a microtubule-dependent transport pathway. Nuclear pore complexes (NPCs) appear to selectively control the transport of iridium(III) complexes between the cytoplasm and nucleus. A generalization of trends in behavior and structure-activity relationships is presented, which should provide further insights into the design and optimization of future probes. Author Affiliation: Department of Chemistry & State Key Laboratory of Molecular Engineering of Polymers, Fudan University, 220 Handan Road, Shanghai 200433, PR China Article History: Received 25 August 2012; Accepted 10 September 2012
language: eng
source:
identifier: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2012.09.014
fulltext: fulltext
issn:
  • 0142-9612
  • 01429612
  • 1878-5905
  • 18785905
url: Link


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