schliessen

Filtern

 

Bibliotheken

Inactivation of encapsulated cells and their therapeutic effects by means of TGL triple-fusion reporter/biosafety gene

The immobilization of cells within alginate-poly- -lysine-alginate (APA) microcapsules has been demonstrated to be an effective technology design for long term delivery of therapeutic products. Despite promising advances, biosafety aspects still remain to be improved. Here, we describe a complete ch... Full description

Journal Title: Biomaterials January 2013, Vol.34(4), pp.1442-1451
Main Author: Santos, Edorta
Other Authors: Larzabal, Leyre , Calvo, Alfonso , Orive, Gorka , Pedraz, José Luis , Hernández, Rosa M
Format: Electronic Article Electronic Article
Language: English
Subjects:
Tgl
ID: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2012.10.076
Link: https://www.sciencedirect.com/science/article/pii/S0142961212012422
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: elsevier_sdoi_10_1016_j_biomaterials_2012_10_076
title: Inactivation of encapsulated cells and their therapeutic effects by means of TGL triple-fusion reporter/biosafety gene
format: Article
creator:
  • Santos, Edorta
  • Larzabal, Leyre
  • Calvo, Alfonso
  • Orive, Gorka
  • Pedraz, José Luis
  • Hernández, Rosa M
subjects:
  • Microcapsule
  • Tgl
  • Drug Delivery
  • Biosafety
  • Hsv-Tk
  • Medicine
  • Engineering
ispartof: Biomaterials, January 2013, Vol.34(4), pp.1442-1451
description: The immobilization of cells within alginate-poly- -lysine-alginate (APA) microcapsules has been demonstrated to be an effective technology design for long term delivery of therapeutic products. Despite promising advances, biosafety aspects still remain to be improved. Here, we describe a complete characterization of the strategy based on TGL triple-fusion reporter gene – which codifies for Herpes Simplex virus type 1 thymidine-kinase (HSV1-TK), green fluorescent protein (GFP) and Firefly Luciferase – (SFG TGL) to inactivate encapsulated cells and their therapeutic effects. Myoblasts genetically engineered to secrete erythropoietin (EPO) were retroviraly transduced with the SFG TGL plasmid to further characterize their ganciclovir (GCV)-mediated inactivation process. GCV sensitivity of encapsulated cells was 100-fold lower when compared to cells plated onto 2D surfaces. However, the number of cells per capsule and EPO secretion decayed to less than 15% at the same time that proliferation was arrested after 14 days of GCV treatment in vitro. In vivo, ten days of GCV treatment was enough to restore the increased hematocrit levels of mice implanted with encapsulated TGL-expressing and EPO-secreting cells. Altogether, these results show that TGL triple-fusion reporter gene may be a good starting point in the search of a suitable biosafety strategy to inactivate encapsulated cells and control their therapeutic effects.
language: eng
source:
identifier: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2012.10.076
fulltext: fulltext
issn:
  • 0142-9612
  • 01429612
  • 1878-5905
  • 18785905
url: Link


@attributes
ID1094156449
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordiddoi_10_1016_j_biomaterials_2012_10_076
sourceidelsevier_s
recordidTN_elsevier_sdoi_10_1016_j_biomaterials_2012_10_076
sourcesystemOther
dbid
0--K
1--M
2.FO
3.~1
41B1
51P~
61RT
71~.
8457
94G.
107-5
118P~
129JM
139JN
14AABNK
15AAEDT
16AAEPC
17AAKOC
18AAOAW
19AAQFI
20ABFNM
21ABGSF
22ABNUV
23ABXRA
24ABYKQ
25ACDAQ
26ACIUM
27ACRLP
28ADEWK
29ADTZH
30ADUVX
31AECPX
32AEHWI
33AEKER
34AEVXI
35AEZYN
36AFKWA
37AFTJW
38AFXIZ
39AGHFR
40AGRDE
41AGUBO
42AGYEJ
43AHHHB
44AHPOS
45AIKHN
46AITUG
47AJBFU
48AJOXV
49AJUYK
50AMFUW
51BJAXD
52BLXMC
53DOVZS
54ENUVR
55EO8
56EO9
57EP2
58EP3
59FDB
60FGOYB
61FIRID
62FNPLU
63G-Q
64GBLVA
65HMK
66HMO
67J1W
68JJJVA
69KOM
70MAGPM
71OAUVE
72OB-
73OM.
74P-8
75P-9
76PC.
77Q38
78R2-
79RPZ
80SAE
81SCC
82SDF
83SDG
84SDP
85SES
86SEW
87SMS
88SPC
89SSG
90SSM
91SST
92SSU
93SSZ
94T5K
95Z5R
96~G-
pqid1664201763
galeid310714787
display
typearticle
titleInactivation of encapsulated cells and their therapeutic effects by means of TGL triple-fusion reporter/biosafety gene
creatorSantos, Edorta ; Larzabal, Leyre ; Calvo, Alfonso ; Orive, Gorka ; Pedraz, José Luis ; Hernández, Rosa M
ispartofBiomaterials, January 2013, Vol.34(4), pp.1442-1451
identifier
subjectMicrocapsule ; Tgl ; Drug Delivery ; Biosafety ; Hsv-Tk ; Medicine ; Engineering
descriptionThe immobilization of cells within alginate-poly- -lysine-alginate (APA) microcapsules has been demonstrated to be an effective technology design for long term delivery of therapeutic products. Despite promising advances, biosafety aspects still remain to be improved. Here, we describe a complete characterization of the strategy based on TGL triple-fusion reporter gene – which codifies for Herpes Simplex virus type 1 thymidine-kinase (HSV1-TK), green fluorescent protein (GFP) and Firefly Luciferase – (SFG TGL) to inactivate encapsulated cells and their therapeutic effects. Myoblasts genetically engineered to secrete erythropoietin (EPO) were retroviraly transduced with the SFG TGL plasmid to further characterize their ganciclovir (GCV)-mediated inactivation process. GCV sensitivity of encapsulated cells was 100-fold lower when compared to cells plated onto 2D surfaces. However, the number of cells per capsule and EPO secretion decayed to less than 15% at the same time that proliferation was arrested after 14 days of GCV treatment in vitro. In vivo, ten days of GCV treatment was enough to restore the increased hematocrit levels of mice implanted with encapsulated TGL-expressing and EPO-secreting cells. Altogether, these results show that TGL triple-fusion reporter gene may be a good starting point in the search of a suitable biosafety strategy to inactivate encapsulated cells and control their therapeutic effects.
languageeng
source
version7
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961212012422$$EView_record_in_ScienceDirect_(Access_to_full_text_may_be_restricted)
search
creatorcontrib
0Santos, Edorta
1Larzabal, Leyre
2Calvo, Alfonso
3Orive, Gorka
4Pedraz, José Luis
5Hernández, Rosa M
titleInactivation of encapsulated cells and their therapeutic effects by means of TGL triple-fusion reporter/biosafety gene
description
subject
0Microcapsule
1Tgl
2Drug Delivery
3Biosafety
4Hsv-Tk
5Medicine
6Engineering
general
0English
1Elsevier Ltd
210.1016/j.biomaterials.2012.10.076
3ScienceDirect (Elsevier B.V.)
4ScienceDirect Journals (Elsevier)
sourceidelsevier_s
recordidelsevier_sdoi_10_1016_j_biomaterials_2012_10_076
issn
00142-9612
101429612
21878-5905
318785905
rsrctypearticle
creationdate2013
addtitleBiomaterials
searchscope
0elsevier_full
1elsevier2
scope
0elsevier_full
1elsevier2
lsr44$$EView_record_in_ScienceDirect_(Access_to_full_text_may_be_restricted)
tmp01ScienceDirect Journals (Elsevier)
tmp02
0--K
1--M
2.FO
3.~1
41B1
51P~
61RT
71~.
8457
94G.
107-5
118P~
129JM
139JN
14AABNK
15AAEDT
16AAEPC
17AAKOC
18AAOAW
19AAQFI
20ABFNM
21ABGSF
22ABNUV
23ABXRA
24ABYKQ
25ACDAQ
26ACIUM
27ACRLP
28ADEWK
29ADTZH
30ADUVX
31AECPX
32AEHWI
33AEKER
34AEVXI
35AEZYN
36AFKWA
37AFTJW
38AFXIZ
39AGHFR
40AGRDE
41AGUBO
42AGYEJ
43AHHHB
44AHPOS
45AIKHN
46AITUG
47AJBFU
48AJOXV
49AJUYK
50AMFUW
51BJAXD
52BLXMC
53DOVZS
54ENUVR
55EO8
56EO9
57EP2
58EP3
59FDB
60FGOYB
61FIRID
62FNPLU
63G-Q
64GBLVA
65HMK
66HMO
67J1W
68JJJVA
69KOM
70MAGPM
71OAUVE
72OB-
73OM.
74P-8
75P-9
76PC.
77Q38
78R2-
79RPZ
80SAE
81SCC
82SDF
83SDG
84SDP
85SES
86SEW
87SMS
88SPC
89SSG
90SSM
91SST
92SSU
93SSZ
94T5K
95Z5R
96~G-
startdate20130101
enddate20130131
lsr40Biomaterials, January 2013, Vol.34 (4), pp.1442-1451
doi10.1016/j.biomaterials.2012.10.076
citationpf 1442 pt 1451 vol 34 issue 4
lsr30VSR-Enriched:[pqid, galeid]
sort
titleInactivation of encapsulated cells and their therapeutic effects by means of TGL triple-fusion reporter/biosafety gene
authorSantos, Edorta ; Larzabal, Leyre ; Calvo, Alfonso ; Orive, Gorka ; Pedraz, José Luis ; Hernández, Rosa M
creationdate20130100
lso0120130100
facets
frbrgroupid7191811289333564010
frbrtype5
newrecords20190904
languageeng
topic
0Microcapsule
1Tgl
2Drug Delivery
3Biosafety
4Hsv-Tk
5Medicine
6Engineering
collectionScienceDirect (Elsevier B.V.)
prefilterarticles
rsrctypearticles
creatorcontrib
0Santos, Edorta
1Larzabal, Leyre
2Calvo, Alfonso
3Orive, Gorka
4Pedraz, José Luis
5Hernández, Rosa M
jtitleBiomaterials
creationdate2013
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Santos
1Larzabal
2Calvo
3Orive
4Pedraz
5Hernández
aufirst
0Edorta
1Leyre
2Alfonso
3Gorka
4José Luis
5Rosa M
auinitE
auinit1E
au
0Santos, Edorta
1Larzabal, Leyre
2Calvo, Alfonso
3Orive, Gorka
4Pedraz, José Luis
5Hernández, Rosa M
atitleInactivation of encapsulated cells and their therapeutic effects by means of TGL triple-fusion reporter/biosafety gene
jtitleBiomaterials
risdate201301
volume34
issue4
spage1442
epage1451
pages1442-1451
issn0142-9612
eissn1878-5905
formatjournal
genrearticle
ristypeJOUR
abstract
pubElsevier Ltd
doi10.1016/j.biomaterials.2012.10.076
lad01Biomaterials
date2013-01