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A phosphorescent silver(I)–gold (I) cluster complex that specifically lights up the nucleolus of living cells with FLIM imaging

The phosphorescent silver(I)–gold(I) cluster complex [CAu Ag (dppy) ](BF ) ( ) selectively stains the nucleolus, with a much lower uptake in the nucleus and cytoplasm, and exhibits excellent photostability. This Ag–Au cluster, which has a photoluminescent lifetime of microseconds, is particularly at... Full description

Journal Title: Biomaterials June 2013, Vol.34(17), pp.4284-4295
Main Author: Chen, Min
Other Authors: Lei, Zhen , Feng, Wei , Li, Chunyan , Wang, Quan-Ming , Li, Fuyou
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2013.02.032
Link: https://www.sciencedirect.com/science/article/pii/S0142961213002019
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recordid: elsevier_sdoi_10_1016_j_biomaterials_2013_02_032
title: A phosphorescent silver(I)–gold (I) cluster complex that specifically lights up the nucleolus of living cells with FLIM imaging
format: Article
creator:
  • Chen, Min
  • Lei, Zhen
  • Feng, Wei
  • Li, Chunyan
  • Wang, Quan-Ming
  • Li, Fuyou
subjects:
  • Silver(I)–Gold (I) Cluster Complex
  • Nucleolus
  • Selective Staining
  • Flim Imaging
  • Medicine
  • Engineering
ispartof: Biomaterials, June 2013, Vol.34(17), pp.4284-4295
description: The phosphorescent silver(I)–gold(I) cluster complex [CAu Ag (dppy) ](BF ) ( ) selectively stains the nucleolus, with a much lower uptake in the nucleus and cytoplasm, and exhibits excellent photostability. This Ag–Au cluster, which has a photoluminescent lifetime of microseconds, is particularly attractive as a probe in applications of time-gated microscopy. Investigation of the pathway of cellular entry indicated that permeates the outer membrane and nuclear membrane of living cells through an energy-dependent and non-endocytic route within 10 min. High concentrations of in the nucleolus have been quantified by inductively coupled plasma atomic emission spectroscopy (ICP-AES) and transmission electron microscopy coupled with an energy dispersive X-ray analysis (TEM-EDXA), which also helped to elucidate the mechanism of the...
language: eng
source:
identifier: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2013.02.032
fulltext: fulltext
issn:
  • 0142-9612
  • 01429612
  • 1878-5905
  • 18785905
url: Link


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titleA phosphorescent silver(I)–gold (I) cluster complex that specifically lights up the nucleolus of living cells with FLIM imaging
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descriptionThe phosphorescent silver(I)–gold(I) cluster complex [CAu Ag (dppy) ](BF ) ( ) selectively stains the nucleolus, with a much lower uptake in the nucleus and cytoplasm, and exhibits excellent photostability. This Ag–Au cluster, which has a photoluminescent lifetime of microseconds, is particularly attractive as a probe in applications of time-gated microscopy. Investigation of the pathway of cellular entry indicated that permeates the outer membrane and nuclear membrane of living cells through an energy-dependent and non-endocytic route within 10 min. High concentrations of in the nucleolus have been quantified by inductively coupled plasma atomic emission spectroscopy (ICP-AES) and transmission electron microscopy coupled with an energy dispersive X-ray analysis (TEM-EDXA), which also helped to elucidate the mechanism of the...
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