schliessen

Filtern

 

Bibliotheken

Effect of binding affinity for siRNA on the in vivo antitumor efficacy of polyplexes

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.biomaterials.2013.03.060 Byline: Lu Han, Cui Tang, Chunhua Yin Abstract: To elucidate the effect of binding affinity for siRNA on the in vivo antitumor efficacy of polyplexes, five kinds of galactose modified... Full description

Journal Title: Biomaterials July 2013, Vol.34(21), pp.5317-5327
Main Author: Han, Lu
Other Authors: Tang, Cui , Yin, Chunhua
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2013.03.060
Link: https://www.sciencedirect.com/science/article/pii/S0142961213003827
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: elsevier_sdoi_10_1016_j_biomaterials_2013_03_060
title: Effect of binding affinity for siRNA on the in vivo antitumor efficacy of polyplexes
format: Article
creator:
  • Han, Lu
  • Tang, Cui
  • Yin, Chunhua
subjects:
  • Sirna Delivery
  • Binding Affinity
  • Extracellular Stability
  • Intracellular Dissociation
  • Antitumor Efficacy
  • Medicine
  • Engineering
ispartof: Biomaterials, July 2013, Vol.34(21), pp.5317-5327
description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.biomaterials.2013.03.060 Byline: Lu Han, Cui Tang, Chunhua Yin Abstract: To elucidate the effect of binding affinity for siRNA on the in vivo antitumor efficacy of polyplexes, five kinds of galactose modified trimethyl chitosan-cysteine (GTC) conjugate-based polyplexes were developed through adjusting the incorporated ionic crosslinkers. The resultant polyplexes exhibited similar particle size (135-170 nm) and zeta potential (30-35 mV). Their distinct binding affinities for siRNA were evaluated by gel retardation, heparin displacement, and in vitro siRNA release assays. GTC polyplexes with weak polymer-siRNA binding were structurally unstable and highly susceptible to nuclease degradation, resulting in poor cellular uptake. However, strong binding affinity for siRNA was correlated to delayed intracellular dissociation of polyplexes. The polyplexes with optimized binding affinity for siRNA provided enough protection of siRNA prior to releasing it efficiently in the cytoplasm, resulting in efficient and persistent gene knockdown in vitro. Furthermore, they had remarkable antitumor efficacy in vivo with regard to the tumor growth retardation, gene knockdown, angiogenesis inhibition, and apoptosis induction in QGY-7703 tumor bearing mice. Therefore, tailoring of the binding strength between the polymeric vector and its siRNA cargo in polyplexes may serve as a feasible tool for improving their therapeutic efficacy. Author Affiliation: State Key Laboratory of Genetic Engineering, Department of Pharmaceutical Sciences, School of Life Sciences, Fudan University, Shanghai 200433, China Article History: Received 4 February 2013; Accepted 20 March 2013
language: eng
source:
identifier: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2013.03.060
fulltext: fulltext
issn:
  • 0142-9612
  • 01429612
  • 1878-5905
  • 18785905
url: Link


@attributes
ID1147695593
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordiddoi_10_1016_j_biomaterials_2013_03_060
sourceidelsevier_s
recordidTN_elsevier_sdoi_10_1016_j_biomaterials_2013_03_060
sourcesystemPC
dbid
0--K
1--M
2.FO
3.~1
41B1
51P~
61RT
71~.
8457
94G.
107-5
118P~
129JM
139JN
14AABNK
15AAEDT
16AAEPC
17AAKOC
18AAOAW
19AAQFI
20ABFNM
21ABGSF
22ABNUV
23ABXRA
24ABYKQ
25ACDAQ
26ACIUM
27ACRLP
28ADEWK
29ADTZH
30ADUVX
31AECPX
32AEHWI
33AEKER
34AEVXI
35AEZYN
36AFKWA
37AFTJW
38AFXIZ
39AGHFR
40AGRDE
41AGUBO
42AGYEJ
43AHHHB
44AHPOS
45AIKHN
46AITUG
47AJBFU
48AJOXV
49AJUYK
50AMFUW
51BJAXD
52BLXMC
53DOVZS
54ENUVR
55EO8
56EO9
57EP2
58EP3
59FDB
60FGOYB
61FIRID
62FNPLU
63G-Q
64GBLVA
65HMK
66HMO
67J1W
68JJJVA
69KOM
70MAGPM
71OAUVE
72OB-
73OM.
74P-8
75P-9
76PC.
77Q38
78R2-
79RPZ
80SAE
81SCC
82SDF
83SDG
84SDP
85SES
86SEW
87SMS
88SPC
89SSG
90SSM
91SST
92SSU
93SSZ
94T5K
95Z5R
96~G-
pqid1349092048
galeid333595299
display
typearticle
titleEffect of binding affinity for siRNA on the in vivo antitumor efficacy of polyplexes
creatorHan, Lu ; Tang, Cui ; Yin, Chunhua
ispartofBiomaterials, July 2013, Vol.34(21), pp.5317-5327
identifier
subjectSirna Delivery ; Binding Affinity ; Extracellular Stability ; Intracellular Dissociation ; Antitumor Efficacy ; Medicine ; Engineering
languageeng
source
descriptionTo link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.biomaterials.2013.03.060 Byline: Lu Han, Cui Tang, Chunhua Yin Abstract: To elucidate the effect of binding affinity for siRNA on the in vivo antitumor efficacy of polyplexes, five kinds of galactose modified trimethyl chitosan-cysteine (GTC) conjugate-based polyplexes were developed through adjusting the incorporated ionic crosslinkers. The resultant polyplexes exhibited similar particle size (135-170 nm) and zeta potential (30-35 mV). Their distinct binding affinities for siRNA were evaluated by gel retardation, heparin displacement, and in vitro siRNA release assays. GTC polyplexes with weak polymer-siRNA binding were structurally unstable and highly susceptible to nuclease degradation, resulting in poor cellular uptake. However, strong binding affinity for siRNA was correlated to delayed intracellular dissociation of polyplexes. The polyplexes with optimized binding affinity for siRNA provided enough protection of siRNA prior to releasing it efficiently in the cytoplasm, resulting in efficient and persistent gene knockdown in vitro. Furthermore, they had remarkable antitumor efficacy in vivo with regard to the tumor growth retardation, gene knockdown, angiogenesis inhibition, and apoptosis induction in QGY-7703 tumor bearing mice. Therefore, tailoring of the binding strength between the polymeric vector and its siRNA cargo in polyplexes may serve as a feasible tool for improving their therapeutic efficacy. Author Affiliation: State Key Laboratory of Genetic Engineering, Department of Pharmaceutical Sciences, School of Life Sciences, Fudan University, Shanghai 200433, China Article History: Received 4 February 2013; Accepted 20 March 2013
version7
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961213003827$$EView_record_in_ScienceDirect_(Access_to_full_text_may_be_restricted)
search
creatorcontrib
0Han, Lu
1Tang, Cui
2Yin, Chunhua
titleEffect of binding affinity for siRNA on the in vivo antitumor efficacy of polyplexes
description

To elucidate the effect of binding affinity for siRNA on the

antitumor efficacy of polyplexes, five kinds of galactose modified trimethyl chitosan-cysteine (GTC) conjugate-based polyplexes were developed through adjusting the incorporated ionic crosslinkers. The resultant polyplexes exhibited similar particle size (135–170 nm) and zeta potential (30–35 mV). Their distinct binding affinities for siRNA were evaluated by gel retardation, heparin displacement, and

siRNA release assays. GTC polyplexes with weak polymer-siRNA binding were structurally unstable and highly susceptible to nuclease degradation, resulting in poor cellular uptake. However, strong binding affinity for siRNA was correlated to delayed intracellular dissociation of polyplexes. The polyplexes with optimized binding affinity for siRNA provided enough protection of siRNA prior to releasing it efficiently in the cytoplasm, resulting in efficient and persistent gene knockdown...

subject
0Sirna Delivery
1Binding Affinity
2Extracellular Stability
3Intracellular Dissociation
4Antitumor Efficacy
5Medicine
6Engineering
general
0English
1Elsevier Ltd
210.1016/j.biomaterials.2013.03.060
3ScienceDirect (Elsevier)
4ScienceDirect Journals (Elsevier)
sourceidelsevier_s
recordidelsevier_sdoi_10_1016_j_biomaterials_2013_03_060
issn
00142-9612
101429612
21878-5905
318785905
rsrctypearticle
creationdate2013
addtitleBiomaterials
searchscope
0elsevier_full
1elsevier2
scope
0elsevier_full
1elsevier2
lsr44$$EView_record_in_ScienceDirect_(Access_to_full_text_may_be_restricted)
tmp01ScienceDirect Journals (Elsevier)
tmp02
0--K
1--M
2.FO
3.~1
41B1
51P~
61RT
71~.
8457
94G.
107-5
118P~
129JM
139JN
14AABNK
15AAEDT
16AAEPC
17AAKOC
18AAOAW
19AAQFI
20ABFNM
21ABGSF
22ABNUV
23ABXRA
24ABYKQ
25ACDAQ
26ACIUM
27ACRLP
28ADEWK
29ADTZH
30ADUVX
31AECPX
32AEHWI
33AEKER
34AEVXI
35AEZYN
36AFKWA
37AFTJW
38AFXIZ
39AGHFR
40AGRDE
41AGUBO
42AGYEJ
43AHHHB
44AHPOS
45AIKHN
46AITUG
47AJBFU
48AJOXV
49AJUYK
50AMFUW
51BJAXD
52BLXMC
53DOVZS
54ENUVR
55EO8
56EO9
57EP2
58EP3
59FDB
60FGOYB
61FIRID
62FNPLU
63G-Q
64GBLVA
65HMK
66HMO
67J1W
68JJJVA
69KOM
70MAGPM
71OAUVE
72OB-
73OM.
74P-8
75P-9
76PC.
77Q38
78R2-
79RPZ
80SAE
81SCC
82SDF
83SDG
84SDP
85SES
86SEW
87SMS
88SPC
89SSG
90SSM
91SST
92SSU
93SSZ
94T5K
95Z5R
96~G-
startdate20130701
enddate20130731
lsr40Biomaterials, July 2013, Vol.34 (21), pp.5317-5327
doi10.1016/j.biomaterials.2013.03.060
citationpf 5317 pt 5327 vol 34 issue 21
lsr30VSR-Enriched:[description, galeid, pqid]
sort
titleEffect of binding affinity for siRNA on the in vivo antitumor efficacy of polyplexes
authorHan, Lu ; Tang, Cui ; Yin, Chunhua
creationdate20130700
lso0120130700
facets
frbrgroupid9208616508422194920
frbrtype5
newrecords20190904
languageeng
topic
0Sirna Delivery
1Binding Affinity
2Extracellular Stability
3Intracellular Dissociation
4Antitumor Efficacy
5Medicine
6Engineering
collectionScienceDirect (Elsevier)
prefilterarticles
rsrctypearticles
creatorcontrib
0Han, Lu
1Tang, Cui
2Yin, Chunhua
jtitleBiomaterials
creationdate2013
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Han
1Tang
2Yin
aufirst
0Lu
1Cui
2Chunhua
auinitL
auinit1L
au
0Han, Lu
1Tang, Cui
2Yin, Chunhua
atitleEffect of binding affinity for siRNA on the in vivo antitumor efficacy of polyplexes
jtitleBiomaterials
risdate201307
volume34
issue21
spage5317
epage5327
pages5317-5327
issn0142-9612
eissn1878-5905
formatjournal
genrearticle
ristypeJOUR
abstract

To elucidate the effect of binding affinity for siRNA on the

antitumor efficacy of polyplexes, five kinds of galactose modified trimethyl chitosan-cysteine (GTC) conjugate-based polyplexes were developed through adjusting the incorporated ionic crosslinkers. The resultant polyplexes exhibited similar particle size (135–170 nm) and zeta potential (30–35 mV). Their distinct binding affinities for siRNA were evaluated by gel retardation, heparin displacement, and

siRNA release assays. GTC polyplexes with weak polymer-siRNA binding were structurally unstable and highly susceptible to nuclease degradation, resulting in poor cellular uptake. However, strong binding affinity for siRNA was correlated to delayed intracellular dissociation of polyplexes. The polyplexes with optimized binding affinity for siRNA provided enough protection of siRNA prior to releasing it efficiently in the cytoplasm, resulting in efficient and persistent gene knockdown...

pubElsevier Ltd
doi10.1016/j.biomaterials.2013.03.060
lad01Biomaterials
date2013-07