schliessen

Filtern

 

Bibliotheken

Magnetization transfer contrast MRI for non-invasive assessment of innate and adaptive immune responses against alginate-encapsulated cells

By means of physical isolation of cells inside semi-permeable hydrogels, encapsulation has been widely used to immunoprotect transplanted cells. While spherical alginate microcapsules are now being used clinically, there still is little known about the patient's immune system response unless biopsie... Full description

Journal Title: Biomaterials September 2014, Vol.35(27), pp.7811-7818
Main Author: Chan, Kannie W.Y
Other Authors: Liu, Guanshu , van Zijl, Peter C.M , Bulte, Jeff W.M , Mcmahon, Michael T
Format: Electronic Article Electronic Article
Language: English
Subjects:
Mri
ID: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2014.05.057
Link: https://www.sciencedirect.com/science/article/pii/S0142961214006140
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: elsevier_sdoi_10_1016_j_biomaterials_2014_05_057
title: Magnetization transfer contrast MRI for non-invasive assessment of innate and adaptive immune responses against alginate-encapsulated cells
format: Article
creator:
  • Chan, Kannie W.Y
  • Liu, Guanshu
  • van Zijl, Peter C.M
  • Bulte, Jeff W.M
  • Mcmahon, Michael T
subjects:
  • Alginate Microcapsules
  • Cellular Imaging
  • Molecular Imaging
  • Immune Response
  • Mri
  • Medicine
  • Engineering
ispartof: Biomaterials, September 2014, Vol.35(27), pp.7811-7818
description: By means of physical isolation of cells inside semi-permeable hydrogels, encapsulation has been widely used to immunoprotect transplanted cells. While spherical alginate microcapsules are now being used clinically, there still is little known about the patient's immune system response unless biopsies are obtained. We investigated the use of Magnetization Transfer (MT) imaging to non-invasively detect host immune responses against alginate capsules containing xenografted human hepatocytes in four groups of animals, including transplanted empty capsules (−Cells/−IS), capsules with live cells with (+LiveCells/+IS) and without immunosuppression (+LiveCells/−IS), and capsules with apoptotic cells in non-immunosuppressed animals (+DeadCells/−IS). The highest MT ratio (MTR) was found in +LiveCells/−IS, which increased from day 0 by 38% and 53% on days 7 and 14 after transplantation respectively, and corresponded to a distinctive increase in cell infiltration on histology. Furthermore, we show that macromolecular ratio maps based on MT data are more sensitive to cell infiltration and fibrosis than conventional MTR maps. Such maps showed a significant difference between +LiveCells/−IS (0.18 ± 0.02) and +DeadCells/−IS (0.13 ± 0.02) on day 7 (  
language: eng
source:
identifier: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2014.05.057
fulltext: fulltext
issn:
  • 0142-9612
  • 01429612
  • 1878-5905
  • 18785905
url: Link


@attributes
ID1479993027
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordiddoi_10_1016_j_biomaterials_2014_05_057
sourceidelsevier_s
recordidTN_elsevier_sdoi_10_1016_j_biomaterials_2014_05_057
sourcesystemOther
dbid
0--K
1--M
2.FO
3.~1
41B1
51P~
61RT
71~.
8457
94G.
107-5
118P~
129JM
139JN
14AABNK
15AAEDT
16AAEPC
17AAKOC
18AAOAW
19AAQFI
20ABFNM
21ABGSF
22ABNUV
23ABXRA
24ABYKQ
25ACDAQ
26ACIUM
27ACRLP
28ADEWK
29ADTZH
30ADUVX
31AECPX
32AEHWI
33AEKER
34AEVXI
35AEZYN
36AFKWA
37AFTJW
38AFXIZ
39AGHFR
40AGRDE
41AGUBO
42AGYEJ
43AHHHB
44AHPOS
45AIKHN
46AITUG
47AJBFU
48AJOXV
49AJUYK
50AMFUW
51BJAXD
52BLXMC
53DOVZS
54ENUVR
55EO8
56EO9
57EP2
58EP3
59FDB
60FGOYB
61FIRID
62FNPLU
63G-Q
64GBLVA
65HMK
66HMO
67J1W
68JJJVA
69KOM
70MAGPM
71OAUVE
72OB-
73OM.
74P-8
75P-9
76PC.
77Q38
78R2-
79RPZ
80SAE
81SCC
82SDF
83SDG
84SDP
85SES
86SEW
87SMS
88SPC
89SSG
90SSM
91SST
92SSU
93SSZ
94T5K
95Z5R
96~G-
pqid1642251009
galeid519483736
display
typearticle
titleMagnetization transfer contrast MRI for non-invasive assessment of innate and adaptive immune responses against alginate-encapsulated cells
creatorChan, Kannie W.Y ; Liu, Guanshu ; van Zijl, Peter C.M ; Bulte, Jeff W.M ; Mcmahon, Michael T
ispartofBiomaterials, September 2014, Vol.35(27), pp.7811-7818
identifier
subjectAlginate Microcapsules ; Cellular Imaging ; Molecular Imaging ; Immune Response ; Mri ; Medicine ; Engineering
descriptionBy means of physical isolation of cells inside semi-permeable hydrogels, encapsulation has been widely used to immunoprotect transplanted cells. While spherical alginate microcapsules are now being used clinically, there still is little known about the patient's immune system response unless biopsies are obtained. We investigated the use of Magnetization Transfer (MT) imaging to non-invasively detect host immune responses against alginate capsules containing xenografted human hepatocytes in four groups of animals, including transplanted empty capsules (−Cells/−IS), capsules with live cells with (+LiveCells/+IS) and without immunosuppression (+LiveCells/−IS), and capsules with apoptotic cells in non-immunosuppressed animals (+DeadCells/−IS). The highest MT ratio (MTR) was found in +LiveCells/−IS, which increased from day 0 by 38% and 53% on days 7 and 14 after transplantation respectively, and corresponded to a distinctive increase in cell infiltration on histology. Furthermore, we show that macromolecular ratio maps based on MT data are more sensitive to cell infiltration and fibrosis than conventional MTR maps. Such maps showed a significant difference between +LiveCells/−IS (0.18 ± 0.02) and +DeadCells/−IS (0.13 ± 0.02) on day 7 (  < 0.01) existed, which was not observed on MTR imaging. We conclude that MT imaging, which is clinically available, can be applied for non-invasive monitoring of the occurrence of a host immune response against encapsulated cells.
languageeng
source
version8
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961214006140$$EView_record_in_ScienceDirect_(Access_to_full_text_may_be_restricted)
search
creatorcontrib
0Chan, Kannie W.Y
1Liu, Guanshu
2van Zijl, Peter C.M
3Bulte, Jeff W.M
4Mcmahon, Michael T
titleMagnetization transfer contrast MRI for non-invasive assessment of innate and adaptive immune responses against alginate-encapsulated cells
description

By means of physical isolation of cells inside semi-permeable hydrogels, encapsulation has been widely used to immunoprotect transplanted cells. While spherical alginate microcapsules are now being used clinically, there still is little known about the patient's immune system response unless biopsies are obtained. We investigated the use of Magnetization Transfer (MT) imaging to non-invasively detect host immune responses against alginate capsules containing xenografted human hepatocytes in four groups of animals, including transplanted empty capsules (−Cells/−IS), capsules with live cells with (+LiveCells/+IS) and without immunosuppression (+LiveCells/−IS), and capsules with apoptotic cells in non-immunosuppressed animals (+DeadCells/−IS). The highest MT ratio (MTR) was found in +LiveCells/−IS, which increased from day 0 by 38% and 53% on days 7 and 14 after transplantation respectively, and corresponded to a distinctive increase in cell infiltration on histology. Furthermore, we show that macromolecular ratio maps based on MT data are more sensitive to cell infiltration and fibrosis than conventional MTR maps. Such maps showed a significant difference between +LiveCells/−IS (0.18 ± 0.02) and +DeadCells/−IS (0.13 ± 0.02) on day 7 (

 < 0.01) existed, which was not observed on MTR imaging. We conclude that MT imaging, which is clinically available, can be applied for non-invasive monitoring of the occurrence of a host immune response against encapsulated cells.

subject
0Alginate Microcapsules
1Cellular Imaging
2Molecular Imaging
3Immune Response
4Mri
5Medicine
6Engineering
general
0English
1Elsevier Ltd
210.1016/j.biomaterials.2014.05.057
3ScienceDirect (Elsevier B.V.)
4ScienceDirect Journals (Elsevier)
sourceidelsevier_s
recordidelsevier_sdoi_10_1016_j_biomaterials_2014_05_057
issn
00142-9612
101429612
21878-5905
318785905
rsrctypearticle
creationdate2014
addtitleBiomaterials
searchscope
0elsevier_full
1elsevier2
scope
0elsevier_full
1elsevier2
lsr44$$EView_record_in_ScienceDirect_(Access_to_full_text_may_be_restricted)
tmp01ScienceDirect Journals (Elsevier)
tmp02
0--K
1--M
2.FO
3.~1
41B1
51P~
61RT
71~.
8457
94G.
107-5
118P~
129JM
139JN
14AABNK
15AAEDT
16AAEPC
17AAKOC
18AAOAW
19AAQFI
20ABFNM
21ABGSF
22ABNUV
23ABXRA
24ABYKQ
25ACDAQ
26ACIUM
27ACRLP
28ADEWK
29ADTZH
30ADUVX
31AECPX
32AEHWI
33AEKER
34AEVXI
35AEZYN
36AFKWA
37AFTJW
38AFXIZ
39AGHFR
40AGRDE
41AGUBO
42AGYEJ
43AHHHB
44AHPOS
45AIKHN
46AITUG
47AJBFU
48AJOXV
49AJUYK
50AMFUW
51BJAXD
52BLXMC
53DOVZS
54ENUVR
55EO8
56EO9
57EP2
58EP3
59FDB
60FGOYB
61FIRID
62FNPLU
63G-Q
64GBLVA
65HMK
66HMO
67J1W
68JJJVA
69KOM
70MAGPM
71OAUVE
72OB-
73OM.
74P-8
75P-9
76PC.
77Q38
78R2-
79RPZ
80SAE
81SCC
82SDF
83SDG
84SDP
85SES
86SEW
87SMS
88SPC
89SSG
90SSM
91SST
92SSU
93SSZ
94T5K
95Z5R
96~G-
startdate20140901
enddate20140931
lsr40Biomaterials, September 2014, Vol.35 (27), pp.7811-7818
doi10.1016/j.biomaterials.2014.05.057
citationpf 7811 pt 7818 vol 35 issue 27
lsr30VSR-Enriched:[pqid, galeid]
sort
titleMagnetization transfer contrast MRI for non-invasive assessment of innate and adaptive immune responses against alginate-encapsulated cells
authorChan, Kannie W.Y ; Liu, Guanshu ; van Zijl, Peter C.M ; Bulte, Jeff W.M ; Mcmahon, Michael T
creationdate20140900
lso0120140900
facets
frbrgroupid7713092314002798802
frbrtype5
newrecords20190904
languageeng
topic
0Alginate Microcapsules
1Cellular Imaging
2Molecular Imaging
3Immune Response
4Mri
5Medicine
6Engineering
collectionScienceDirect (Elsevier B.V.)
prefilterarticles
rsrctypearticles
creatorcontrib
0Chan, Kannie W.Y
1Liu, Guanshu
2van Zijl, Peter C.M
3Bulte, Jeff W.M
4Mcmahon, Michael T
jtitleBiomaterials
creationdate2014
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Chan
1Liu
2van Zijl
3Bulte
4Mcmahon
aufirst
0Kannie W.Y
1Guanshu
2Peter C.M
3Jeff W.M
4Michael T
auinitK
auinit1K
au
0Chan, Kannie W.Y
1Liu, Guanshu
2van Zijl, Peter C.M
3Bulte, Jeff W.M
4Mcmahon, Michael T
atitleMagnetization transfer contrast MRI for non-invasive assessment of innate and adaptive immune responses against alginate-encapsulated cells
jtitleBiomaterials
risdate201409
volume35
issue27
spage7811
epage7818
pages7811-7818
issn0142-9612
eissn1878-5905
formatjournal
genrearticle
ristypeJOUR
abstract

By means of physical isolation of cells inside semi-permeable hydrogels, encapsulation has been widely used to immunoprotect transplanted cells. While spherical alginate microcapsules are now being used clinically, there still is little known about the patient's immune system response unless biopsies are obtained. We investigated the use of Magnetization Transfer (MT) imaging to non-invasively detect host immune responses against alginate capsules containing xenografted human hepatocytes in four groups of animals, including transplanted empty capsules (−Cells/−IS), capsules with live cells with (+LiveCells/+IS) and without immunosuppression (+LiveCells/−IS), and capsules with apoptotic cells in non-immunosuppressed animals (+DeadCells/−IS). The highest MT ratio (MTR) was found in +LiveCells/−IS, which increased from day 0 by 38% and 53% on days 7 and 14 after transplantation respectively, and corresponded to a distinctive increase in cell infiltration on histology. Furthermore, we show that macromolecular ratio maps based on MT data are more sensitive to cell infiltration and fibrosis than conventional MTR maps. Such maps showed a significant difference between +LiveCells/−IS (0.18 ± 0.02) and +DeadCells/−IS (0.13 ± 0.02) on day 7 (

 < 0.01) existed, which was not observed on MTR imaging. We conclude that MT imaging, which is clinically available, can be applied for non-invasive monitoring of the occurrence of a host immune response against encapsulated cells.

pubElsevier Ltd
doi10.1016/j.biomaterials.2014.05.057
lad01Biomaterials
date2014-09