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c-Kit+ progenitors generate vascular cells for tissue-engineered grafts through modulation of the Wnt/Klf4 pathway

The development of decellularised scaffolds for small diameter vascular grafts is hampered by their limited patency, due to the lack of luminal cell coverage by endothelial cells (EC) and to the low tone of the vessel due to absence of a contractile smooth muscle cells (SMC). In this study, we ident... Full description

Journal Title: Biomaterials August 2015, Vol.60, pp.53-61
Main Author: Campagnolo, Paola
Other Authors: Tsai, Tsung-Neng , Hong, Xuechong , Kirton, John Paul , So, Po-Wah , Margariti, Andriana , Di Bernardini, Elisabetta , Wong, Mei Mei , Hu, Yanhua , Stevens, Molly M , Xu, Qingbo
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2015.04.055
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recordid: elsevier_sdoi_10_1016_j_biomaterials_2015_04_055
title: c-Kit+ progenitors generate vascular cells for tissue-engineered grafts through modulation of the Wnt/Klf4 pathway
format: Article
creator:
  • Campagnolo, Paola
  • Tsai, Tsung-Neng
  • Hong, Xuechong
  • Kirton, John Paul
  • So, Po-Wah
  • Margariti, Andriana
  • Di Bernardini, Elisabetta
  • Wong, Mei Mei
  • Hu, Yanhua
  • Stevens, Molly M
  • Xu, Qingbo
subjects:
  • Vascular Graft
  • Stem Cells
  • Endothelialization
  • Cell Signalling
  • Medicine
  • Engineering
ispartof: Biomaterials, August 2015, Vol.60, pp.53-61
description: The development of decellularised scaffolds for small diameter vascular grafts is hampered by their limited patency, due to the lack of luminal cell coverage by endothelial cells (EC) and to the low tone of the vessel due to absence of a contractile smooth muscle cells (SMC). In this study, we identify a population of vascular progenitor c-Kit+/Sca-1- cells available in large numbers and derived from immuno-privileged embryonic stem cells (ESCs). We also define an efficient and controlled differentiation protocol yielding fully to differentiated ECs and SMCs in sufficient numbers to allow the repopulation of a tissue engineered vascular graft. When seeded on a decellularised vessel, c-Kit+/Sca-1-derived cells recapitulated the native vessel structure and upon implantation in the mouse, markedly reduced neointima formation and mortality, restoring functional vascularisation. We showed that Krüppel-like transcription factor 4 (Klf4) regulates...
language: eng
source:
identifier: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2015.04.055
fulltext: fulltext
issn:
  • 0142-9612
  • 01429612
  • 1878-5905
  • 18785905
url: Link


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titlec-Kit+ progenitors generate vascular cells for tissue-engineered grafts through modulation of the Wnt/Klf4 pathway
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subjectVascular Graft ; Stem Cells ; Endothelialization ; Cell Signalling ; Medicine ; Engineering
descriptionThe development of decellularised scaffolds for small diameter vascular grafts is hampered by their limited patency, due to the lack of luminal cell coverage by endothelial cells (EC) and to the low tone of the vessel due to absence of a contractile smooth muscle cells (SMC). In this study, we identify a population of vascular progenitor c-Kit+/Sca-1- cells available in large numbers and derived from immuno-privileged embryonic stem cells (ESCs). We also define an efficient and controlled differentiation protocol yielding fully to differentiated ECs and SMCs in sufficient numbers to allow the repopulation of a tissue engineered vascular graft. When seeded on a decellularised vessel, c-Kit+/Sca-1-derived cells recapitulated the native vessel structure and upon implantation in the mouse, markedly reduced neointima formation and mortality, restoring functional vascularisation. We showed that Krüppel-like transcription factor 4 (Klf4) regulates...
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titlec-Kit+ progenitors generate vascular cells for tissue-engineered grafts through modulation of the Wnt/Klf4 pathway
description

The development of decellularised scaffolds for small diameter vascular grafts is hampered by their limited patency, due to the lack of luminal cell coverage by endothelial cells (EC) and to the low tone of the vessel due to absence of a contractile smooth muscle cells (SMC). In this study, we identify a population of vascular progenitor c-Kit+/Sca-1- cells available in large numbers and derived from immuno-privileged embryonic stem cells (ESCs). We also define an efficient and controlled differentiation protocol yielding fully to differentiated ECs and SMCs in sufficient numbers to allow the repopulation of a tissue engineered vascular graft. When seeded

on a decellularised vessel, c-Kit+/Sca-1-derived cells recapitulated the native vessel structure and upon

implantation in the mouse, markedly reduced neointima formation and mortality, restoring functional vascularisation. We showed that Krüppel-like transcription factor 4 (Klf4) regulates...

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abstract

The development of decellularised scaffolds for small diameter vascular grafts is hampered by their limited patency, due to the lack of luminal cell coverage by endothelial cells (EC) and to the low tone of the vessel due to absence of a contractile smooth muscle cells (SMC). In this study, we identify a population of vascular progenitor c-Kit+/Sca-1- cells available in large numbers and derived from immuno-privileged embryonic stem cells (ESCs). We also define an efficient and controlled differentiation protocol yielding fully to differentiated ECs and SMCs in sufficient numbers to allow the repopulation of a tissue engineered vascular graft. When seeded

on a decellularised vessel, c-Kit+/Sca-1-derived cells recapitulated the native vessel structure and upon

implantation in the mouse, markedly reduced neointima formation and mortality, restoring functional vascularisation. We showed that Krüppel-like transcription factor 4 (Klf4) regulates...

pubElsevier Ltd
doi10.1016/j.biomaterials.2015.04.055
lad01Biomaterials
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date2015-08