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Effect of NH 4 + and glycerol on cytidine 5′-diphosphocholine synthesis in Saccharomyces cerevisiae

Both stimulation of ammonium ion on the glycolytic flux and regulation by glycerol of enzymes in Kennedy pathway for cytidine diphosphate choline production in were studied. The conventional transformation course featured four stages. Firstly, CMP and choline chloride were phosphorylated and CDP-cho... Full description

Journal Title: Bioresource Technology 2009, Vol.100(20), pp.4848-4853
Main Author: Tang, Jiapeng
Other Authors: Yao, Yuelan , Ying, Hanjie , Xiong, Jian , Zhang, Lei , Li, Zhenjiang , Bai, Jianxin , Zhang, Yeyong , Ouyang, Pingkai
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0960-8524 ; E-ISSN: 1873-2976 ; DOI: 10.1016/j.biortech.2009.04.045
Link: https://www.sciencedirect.com/science/article/pii/S0960852409004544
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recordid: elsevier_sdoi_10_1016_j_biortech_2009_04_045
title: Effect of NH 4 + and glycerol on cytidine 5′-diphosphocholine synthesis in Saccharomyces cerevisiae
format: Article
creator:
  • Tang, Jiapeng
  • Yao, Yuelan
  • Ying, Hanjie
  • Xiong, Jian
  • Zhang, Lei
  • Li, Zhenjiang
  • Bai, Jianxin
  • Zhang, Yeyong
  • Ouyang, Pingkai
subjects:
  • Ammonium Ion
  • Cytidine Diphosphate Choline
  • Glycerol
  • Saccharomyces Cerevisiae
  • Utilization Efficiency of Energy
  • Agriculture
  • Engineering
  • Chemistry
ispartof: Bioresource Technology, 2009, Vol.100(20), pp.4848-4853
description: Both stimulation of ammonium ion on the glycolytic flux and regulation by glycerol of enzymes in Kennedy pathway for cytidine diphosphate choline production in were studied. The conventional transformation course featured four stages. Firstly, CMP and choline chloride were phosphorylated and CDP-choline was formed rapidly; secondly, the rate of CDP-choline formation declined and CMP was not detected in the mixture; thirdly, CMP was released and the CDP-choline concentration reached a peak; Fourthly, the compound concentrations did not practically changes eventually. Using the central composite design, the concentration, yield, and utilization efficiency of energy reached 24.7 mmol/L, 82.3% and 10.6%, with 30 mmol/L of ammonium ion and 1% (V/V) of glycerol, respectively. Ammonium ion not only strengthened the glycolytic pathway, but also coordinated the reaction rate between the glycolytic pathway and the Kennedy pathway. Glycerol alleviated the activity decrease of the key enzymes in the mixture.
language: eng
source:
identifier: ISSN: 0960-8524 ; E-ISSN: 1873-2976 ; DOI: 10.1016/j.biortech.2009.04.045
fulltext: no_fulltext
issn:
  • 0960-8524
  • 09608524
  • 1873-2976
  • 18732976
url: Link


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titleEffect of NH 4 + and glycerol on cytidine 5′-diphosphocholine synthesis in Saccharomyces cerevisiae
creatorTang, Jiapeng ; Yao, Yuelan ; Ying, Hanjie ; Xiong, Jian ; Zhang, Lei ; Li, Zhenjiang ; Bai, Jianxin ; Zhang, Yeyong ; Ouyang, Pingkai
ispartofBioresource Technology, 2009, Vol.100(20), pp.4848-4853
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subjectAmmonium Ion ; Cytidine Diphosphate Choline ; Glycerol ; Saccharomyces Cerevisiae ; Utilization Efficiency of Energy ; Agriculture ; Engineering ; Chemistry
descriptionBoth stimulation of ammonium ion on the glycolytic flux and regulation by glycerol of enzymes in Kennedy pathway for cytidine diphosphate choline production in were studied. The conventional transformation course featured four stages. Firstly, CMP and choline chloride were phosphorylated and CDP-choline was formed rapidly; secondly, the rate of CDP-choline formation declined and CMP was not detected in the mixture; thirdly, CMP was released and the CDP-choline concentration reached a peak; Fourthly, the compound concentrations did not practically changes eventually. Using the central composite design, the concentration, yield, and utilization efficiency of energy reached 24.7 mmol/L, 82.3% and 10.6%, with 30 mmol/L of ammonium ion and 1% (V/V) of glycerol, respectively. Ammonium ion not only strengthened the glycolytic pathway, but also coordinated the reaction rate between the glycolytic pathway and the Kennedy pathway. Glycerol alleviated the activity decrease of the key enzymes in the mixture.
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