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High-nitrate wastewater treatment in an expanded granular sludge bed reactor and microbial diversity using 454 pyrosequencing analysis

► Complete denitrification can be achieved with nitrate nitrogen concentration as high as 14000mg/L. ► The optimal performance parameters were achieved: C/N ratio for 2, Vup for 3.0, pH of 6.2–8.2. ► 454-Pyrosequencing technology was used to analyze bacterial diversity. ► The EGSB bioreactor offers... Full description

Journal Title: Bioresource Technology April 2013, Vol.134, pp.190-197
Main Author: Liao, Runhua
Other Authors: Shen, Ke , Li, Ai-Min , Shi, Peng , Li, Yan , Shi, Qianqian , Wang, Zhu
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0960-8524 ; E-ISSN: 1873-2976 ; DOI: 10.1016/j.biortech.2012.12.057
Link: http://dx.doi.org/10.1016/j.biortech.2012.12.057
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recordid: elsevier_sdoi_10_1016_j_biortech_2012_12_057
title: High-nitrate wastewater treatment in an expanded granular sludge bed reactor and microbial diversity using 454 pyrosequencing analysis
format: Article
creator:
  • Liao, Runhua
  • Shen, Ke
  • Li, Ai-Min
  • Shi, Peng
  • Li, Yan
  • Shi, Qianqian
  • Wang, Zhu
subjects:
  • Denitrification
  • Expanded Granular Sludge Bed (Egsb) Reactor
  • High-Nitrate Waste
  • 454-Pyrosequencing
  • Denitrification
  • Expanded Granular Sludge Bed (Egsb) Reactor
  • High-Nitrate Waste
  • 454-Pyrosequencing
  • Agriculture
  • Engineering
  • Chemistry
ispartof: Bioresource Technology, April 2013, Vol.134, pp.190-197
description: ► Complete denitrification can be achieved with nitrate nitrogen concentration as high as 14000mg/L. ► The optimal performance parameters were achieved: C/N ratio for 2, Vup for 3.0, pH of 6.2–8.2. ► 454-Pyrosequencing technology was used to analyze bacterial diversity. ► The EGSB bioreactor offers bright prospects for the treatment of high strength nitrate wastewater. Denitrification of high concentration of nitrate wastewater was investigated in expanded granular sludge bed (EGSB) reactor with sodium acetate as the carbon source. The optimal parameters were achieved with C/N mole ratio of 2.0, liquid up-flow velocity (Vup) of 3.0m/h and pH of 6.2–8.2. Complete denitrification can be achieved even with nitrate nitrogen concentration as high as 14000mg/L. Furthermore, 454-pyrosequencing technology was used to analyze bacterial diversity. Results showed that a total of 5573 sequences were obtained which could be affiliated to 6 phylogenetic groups, including...
language: eng
source:
identifier: ISSN: 0960-8524 ; E-ISSN: 1873-2976 ; DOI: 10.1016/j.biortech.2012.12.057
fulltext: no_fulltext
issn:
  • 0960-8524
  • 09608524
  • 1873-2976
  • 18732976
url: Link


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subjectDenitrification ; Expanded Granular Sludge Bed (Egsb) Reactor ; High-Nitrate Waste ; 454-Pyrosequencing ; Denitrification ; Expanded Granular Sludge Bed (Egsb) Reactor ; High-Nitrate Waste ; 454-Pyrosequencing ; Agriculture ; Engineering ; Chemistry
description► Complete denitrification can be achieved with nitrate nitrogen concentration as high as 14000mg/L. ► The optimal performance parameters were achieved: C/N ratio for 2, Vup for 3.0, pH of 6.2–8.2. ► 454-Pyrosequencing technology was used to analyze bacterial diversity. ► The EGSB bioreactor offers bright prospects for the treatment of high strength nitrate wastewater. Denitrification of high concentration of nitrate wastewater was investigated in expanded granular sludge bed (EGSB) reactor with sodium acetate as the carbon source. The optimal parameters were achieved with C/N mole ratio of 2.0, liquid up-flow velocity (Vup) of 3.0m/h and pH of 6.2–8.2. Complete denitrification can be achieved even with nitrate nitrogen concentration as high as 14000mg/L. Furthermore, 454-pyrosequencing technology was used to analyze bacterial diversity. Results showed that a total of 5573 sequences were obtained which could be affiliated to 6 phylogenetic groups, including...
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► Complete denitrification can be achieved with nitrate nitrogen concentration as high as 14000mg/L. ► The optimal performance parameters were achieved: C/N ratio for 2, Vup for 3.0, pH of 6.2–8.2. ► 454-Pyrosequencing technology was used to analyze bacterial diversity. ► The EGSB bioreactor offers bright prospects for the treatment of high strength nitrate wastewater.

Denitrification of high concentration of nitrate wastewater was investigated in expanded granular sludge bed (EGSB) reactor with sodium acetate as the carbon source. The optimal parameters were achieved with C/N mole ratio of 2.0, liquid up-flow velocity (Vup) of 3.0m/h and pH of 6.2–8.2. Complete denitrification can be achieved even with nitrate nitrogen concentration as high as 14000mg/L. Furthermore, 454-pyrosequencing technology was used to analyze bacterial diversity. Results showed that a total of 5573 sequences were obtained which could be affiliated to 6 phylogenetic groups, including...

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► Complete denitrification can be achieved with nitrate nitrogen concentration as high as 14000mg/L. ► The optimal performance parameters were achieved: C/N ratio for 2, Vup for 3.0, pH of 6.2–8.2. ► 454-Pyrosequencing technology was used to analyze bacterial diversity. ► The EGSB bioreactor offers bright prospects for the treatment of high strength nitrate wastewater.

Denitrification of high concentration of nitrate wastewater was investigated in expanded granular sludge bed (EGSB) reactor with sodium acetate as the carbon source. The optimal parameters were achieved with C/N mole ratio of 2.0, liquid up-flow velocity (Vup) of 3.0m/h and pH of 6.2–8.2. Complete denitrification can be achieved even with nitrate nitrogen concentration as high as 14000mg/L. Furthermore, 454-pyrosequencing technology was used to analyze bacterial diversity. Results showed that a total of 5573 sequences were obtained which could be affiliated to 6 phylogenetic groups, including...

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lad01Bioresource Technology
date2013-04