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WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins

The lipoglycoproteins of the WNT family act on seven transmembrane-spanning Class Frizzled receptors. Here, we show that WNT-5A evokes a proliferative response in a mouse microglia-like cell line (N13), which is sensitive to pertussis toxin, thus implicating the involvement of heterotrimeric G prote... Full description

Journal Title: Cellular Signalling March 2011, Vol.23(3), pp.550-554
Main Author: Kilander, Michaela Bc
Other Authors: Dijksterhuis, Jacomijn P , Ganji, Ranjani Sri , Bryja, Vitezslav , Schulte, Gunnar
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0898-6568 ; E-ISSN: 1873-3913 ; DOI: 10.1016/j.cellsig.2010.11.004
Link: https://www.sciencedirect.com/science/article/pii/S0898656810003165
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recordid: elsevier_sdoi_10_1016_j_cellsig_2010_11_004
title: WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins
format: Article
creator:
  • Kilander, Michaela Bc
  • Dijksterhuis, Jacomijn P
  • Ganji, Ranjani Sri
  • Bryja, Vitezslav
  • Schulte, Gunnar
subjects:
  • Frizzled
  • Gpcr
  • G Protein
  • [Γ-S]Gtp
  • Non-Canonical
  • Wnt Signaling
  • Biology
ispartof: Cellular Signalling, March 2011, Vol.23(3), pp.550-554
description: The lipoglycoproteins of the WNT family act on seven transmembrane-spanning Class Frizzled receptors. Here, we show that WNT-5A evokes a proliferative response in a mouse microglia-like cell line (N13), which is sensitive to pertussis toxin, thus implicating the involvement of heterotrimeric G proteins of the G family. We continue to show that WNT-5A stimulation of N13 membranes and permeabilized cells evokes the exchange of GDP for GTP of pertussis toxin-sensitive G proteins employing [γ- S]GTP assay and activity state-specific antibodies to GTP-bound G proteins. Our functional analysis of the PTX-sensitivity of WNT-induced G protein activation and PCR analysis of G protein and FZD expression patterns suggest that WNT-5A stimulation leads to the activation of G proteins in N13 cells possibly mediated by FZD , the predominant FZD expressed. In summary, we provide for the first time...
language: eng
source:
identifier: ISSN: 0898-6568 ; E-ISSN: 1873-3913 ; DOI: 10.1016/j.cellsig.2010.11.004
fulltext: fulltext
issn:
  • 0898-6568
  • 08986568
  • 1873-3913
  • 18733913
url: Link


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titleWNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins
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subjectFrizzled ; Gpcr ; G Protein ; [Γ-S]Gtp ; Non-Canonical ; Wnt Signaling ; Biology
descriptionThe lipoglycoproteins of the WNT family act on seven transmembrane-spanning Class Frizzled receptors. Here, we show that WNT-5A evokes a proliferative response in a mouse microglia-like cell line (N13), which is sensitive to pertussis toxin, thus implicating the involvement of heterotrimeric G proteins of the G family. We continue to show that WNT-5A stimulation of N13 membranes and permeabilized cells evokes the exchange of GDP for GTP of pertussis toxin-sensitive G proteins employing [γ- S]GTP assay and activity state-specific antibodies to GTP-bound G proteins. Our functional analysis of the PTX-sensitivity of WNT-induced G protein activation and PCR analysis of G protein and FZD expression patterns suggest that WNT-5A stimulation leads to the activation of G proteins in N13 cells possibly mediated by FZD , the predominant FZD expressed. In summary, we provide for the first time...
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