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A hydrogen peroxide biosensor based on the direct electron transfer of hemoglobin encapsulated in liquid-crystalline cubic phase on electrode

Liquid crystal cubic phase formed with monoolein has been used as immobilizing matrix to host redox protein hemoglobin on glassy carbon electrode surface. The promoted direct electron transfer between hemoglobin and electrode was observed and a large average kinetic electron transfer rate constant k... Full description

Journal Title: Colloids and Surfaces B: Biointerfaces 2011, Vol.82(2), pp.359-364
Main Author: Gao, Feng
Other Authors: Yao, Zhen , Huang, Qin’an , Chen, Xiaoxiao , Guo, Xinyin , Ye, Qingqing , Wang, Lun
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0927-7765 ; E-ISSN: 1873-4367 ; DOI: 10.1016/j.colsurfb.2010.09.009
Link: http://dx.doi.org/10.1016/j.colsurfb.2010.09.009
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recordid: elsevier_sdoi_10_1016_j_colsurfb_2010_09_009
title: A hydrogen peroxide biosensor based on the direct electron transfer of hemoglobin encapsulated in liquid-crystalline cubic phase on electrode
format: Article
creator:
  • Gao, Feng
  • Yao, Zhen
  • Huang, Qin’an
  • Chen, Xiaoxiao
  • Guo, Xinyin
  • Ye, Qingqing
  • Wang, Lun
subjects:
  • Cubic Phase
  • Monoolein
  • Hemoglobin
  • Bioelectrocatalysis
  • Direct Electron Transfer
  • Biosensor
  • Hydrogen Peroxide
  • Cubic Phase
  • Monoolein
  • Hemoglobin
  • Bioelectrocatalysis
  • Direct Electron Transfer
  • Biosensor
  • Hydrogen Peroxide
  • Engineering
  • Chemistry
  • Anatomy & Physiology
ispartof: Colloids and Surfaces B: Biointerfaces, 2011, Vol.82(2), pp.359-364
description: Liquid crystal cubic phase formed with monoolein has been used as immobilizing matrix to host redox protein hemoglobin on glassy carbon electrode surface. The promoted direct electron transfer between hemoglobin and electrode was observed and a large average kinetic electron transfer rate constant k s of 3.03(±0.02) s −1 was estimated. The electrode modified with cubic phase containing hemoglobin retains the bioactivity of hemoglobin and shows excellent bioelectrocatalytic activity to the reduction of hydrogen peroxide with a small apparent Michaelis–Menten constant of 0.25(±0.03) mM. A novel reagentless hydrogen peroxide biosensor was constructed using the hemoglobin-containing cubic phase modified electrode and the proposed hydrogen peroxide biosensor shows a linear range of 7.0–239 μM with a detection limit of 3.1(±0.2) μM and good stability and reproducibility.
language: eng
source:
identifier: ISSN: 0927-7765 ; E-ISSN: 1873-4367 ; DOI: 10.1016/j.colsurfb.2010.09.009
fulltext: fulltext
issn:
  • 0927-7765
  • 09277765
  • 1873-4367
  • 18734367
url: Link


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titleA hydrogen peroxide biosensor based on the direct electron transfer of hemoglobin encapsulated in liquid-crystalline cubic phase on electrode
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descriptionLiquid crystal cubic phase formed with monoolein has been used as immobilizing matrix to host redox protein hemoglobin on glassy carbon electrode surface. The promoted direct electron transfer between hemoglobin and electrode was observed and a large average kinetic electron transfer rate constant k s of 3.03(±0.02) s −1 was estimated. The electrode modified with cubic phase containing hemoglobin retains the bioactivity of hemoglobin and shows excellent bioelectrocatalytic activity to the reduction of hydrogen peroxide with a small apparent Michaelis–Menten constant of 0.25(±0.03) mM. A novel reagentless hydrogen peroxide biosensor was constructed using the hemoglobin-containing cubic phase modified electrode and the proposed hydrogen peroxide biosensor shows a linear range of 7.0–239 μM with a detection limit of 3.1(±0.2) μM and good stability and reproducibility.
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Liquid crystal cubic phase formed with monoolein has been used as immobilizing matrix to host redox protein hemoglobin on glassy carbon electrode surface. The promoted direct electron transfer between hemoglobin and electrode was observed and a large average kinetic electron transfer rate constant k s of 3.03(±0.02) s −1 was estimated. The electrode modified with cubic phase containing hemoglobin retains the bioactivity of hemoglobin and shows excellent bioelectrocatalytic activity to the reduction of hydrogen peroxide with a small apparent Michaelis–Menten constant of 0.25(±0.03) mM. A novel reagentless hydrogen peroxide biosensor was constructed using the hemoglobin-containing cubic phase modified electrode and the proposed hydrogen peroxide biosensor shows a linear range of 7.0–239 μM with a detection limit of 3.1(±0.2) μM and good stability and reproducibility.

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abstract

Liquid crystal cubic phase formed with monoolein has been used as immobilizing matrix to host redox protein hemoglobin on glassy carbon electrode surface. The promoted direct electron transfer between hemoglobin and electrode was observed and a large average kinetic electron transfer rate constant k s of 3.03(±0.02) s −1 was estimated. The electrode modified with cubic phase containing hemoglobin retains the bioactivity of hemoglobin and shows excellent bioelectrocatalytic activity to the reduction of hydrogen peroxide with a small apparent Michaelis–Menten constant of 0.25(±0.03) mM. A novel reagentless hydrogen peroxide biosensor was constructed using the hemoglobin-containing cubic phase modified electrode and the proposed hydrogen peroxide biosensor shows a linear range of 7.0–239 μM with a detection limit of 3.1(±0.2) μM and good stability and reproducibility.

pubElsevier B.V
doi10.1016/j.colsurfb.2010.09.009
lad01Colloids and Surfaces B: Biointerfaces
date2011-02-01