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Protection effect of [Gly14]-Humanin from apoptosis induced by high glucose in human umbilical vein endothelial cells

Humanin (HN) is known for its anti-apoptotic functions in neuronal cells. In this study, we sought to investigate the protective effect of [Gly14]-Humanin (HNG) in high glucose (HG)-induced apoptosis of human umbilical vein endothelial cells (HUVECs). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazo... Full description

Journal Title: Diabetes Research and Clinical Practice December 2014, Vol.106(3), pp.560-566
Main Author: Xie, Ying
Other Authors: Liu, Zhi-Hua , Li, Xiao-Yun , Zhou, Yan-de , Xu, Xingshun , Hu, Li-Fang , Zhang, Yan-Lin , Liu, Chun-Feng
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0168-8227 ; E-ISSN: 1872-8227 ; DOI: 10.1016/j.diabres.2014.09.020
Link: http://dx.doi.org/10.1016/j.diabres.2014.09.020
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recordid: elsevier_sdoi_10_1016_j_diabres_2014_09_020
title: Protection effect of [Gly14]-Humanin from apoptosis induced by high glucose in human umbilical vein endothelial cells
format: Article
creator:
  • Xie, Ying
  • Liu, Zhi-Hua
  • Li, Xiao-Yun
  • Zhou, Yan-de
  • Xu, Xingshun
  • Hu, Li-Fang
  • Zhang, Yan-Lin
  • Liu, Chun-Feng
subjects:
  • Humanin
  • Apoptosis
  • High Glucose
  • Huvecs
  • Humanin
  • Apoptosis
  • High Glucose
  • Huvecs
  • Medicine
ispartof: Diabetes Research and Clinical Practice, December 2014, Vol.106(3), pp.560-566
description: Humanin (HN) is known for its anti-apoptotic functions in neuronal cells. In this study, we sought to investigate the protective effect of [Gly14]-Humanin (HNG) in high glucose (HG)-induced apoptosis of human umbilical vein endothelial cells (HUVECs). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to examine cell viability, DNA chromatin morphology was assessed using Hoechst 33342 staining, and the generation of intracellular reactive oxygen species (ROS) was assessed using the fluorescent probe dichlorofluorescein diacetate (DCFH-DA). The expression of poly ADP-ribose polymerase (PARP), the pro-apoptotic protein bax and the anti-apoptotic protein bcl-2 were examined using western blot analysis. The mRNA level of bax and bcl-2 were detected by quantitative Real-Time PCR. Compared with treatment with HG 72h, pretreatment with HNG for 3h significantly increased cell viability (P0.001), reduced nuclear fluorescence...
language: eng
source:
identifier: ISSN: 0168-8227 ; E-ISSN: 1872-8227 ; DOI: 10.1016/j.diabres.2014.09.020
fulltext: fulltext
issn:
  • 0168-8227
  • 01688227
  • 1872-8227
  • 18728227
url: Link


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titleProtection effect of [Gly14]-Humanin from apoptosis induced by high glucose in human umbilical vein endothelial cells
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subjectHumanin ; Apoptosis ; High Glucose ; Huvecs ; Humanin ; Apoptosis ; High Glucose ; Huvecs ; Medicine
descriptionHumanin (HN) is known for its anti-apoptotic functions in neuronal cells. In this study, we sought to investigate the protective effect of [Gly14]-Humanin (HNG) in high glucose (HG)-induced apoptosis of human umbilical vein endothelial cells (HUVECs). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to examine cell viability, DNA chromatin morphology was assessed using Hoechst 33342 staining, and the generation of intracellular reactive oxygen species (ROS) was assessed using the fluorescent probe dichlorofluorescein diacetate (DCFH-DA). The expression of poly ADP-ribose polymerase (PARP), the pro-apoptotic protein bax and the anti-apoptotic protein bcl-2 were examined using western blot analysis. The mRNA level of bax and bcl-2 were detected by quantitative Real-Time PCR. Compared with treatment with HG 72h, pretreatment with HNG for 3h significantly increased cell viability (P0.001), reduced nuclear fluorescence...
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