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Newly-derived neuroblastoma cell lines propagated in serum-free media recapitulate the genotype and phenotype of primary neuroblastoma tumours

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.ejca.2013.11.015 Byline: Laurel T. Bate-Eya, Marli E. Ebus, Jan Koster, Ilona J.M. den Hartog, Danny A. Zwijnenburg, Linda Schild, Ida van der Ploeg, M. Emmy M. Dolman, Huib N. Caron, Rogier Versteeg, Jan J. M... Full description

Journal Title: European Journal of Cancer February 2014, Vol.50(3), pp.628-637
Main Author: Bate-Eya, Laurel T
Other Authors: Ebus, Marli E , Koster, Jan , Den Hartog, Ilona J.M , Zwijnenburg, Danny A , Schild, Linda , van Der Ploeg, Ida , Dolman, M. Emmy M , Caron, Huib N , Versteeg, Rogier , Molenaar, Jan J
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0959-8049 ; E-ISSN: 1879-0852 ; DOI: 10.1016/j.ejca.2013.11.015
Link: https://www.sciencedirect.com/science/article/pii/S0959804913010101
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recordid: elsevier_sdoi_10_1016_j_ejca_2013_11_015
title: Newly-derived neuroblastoma cell lines propagated in serum-free media recapitulate the genotype and phenotype of primary neuroblastoma tumours
format: Article
creator:
  • Bate-Eya, Laurel T
  • Ebus, Marli E
  • Koster, Jan
  • Den Hartog, Ilona J.M
  • Zwijnenburg, Danny A
  • Schild, Linda
  • van Der Ploeg, Ida
  • Dolman, M. Emmy M
  • Caron, Huib N
  • Versteeg, Rogier
  • Molenaar, Jan J
subjects:
  • Neuroblastoma
  • Neuroblastoma Tics
  • Acgh
  • Whole Genome Sequencing
  • Principal Component Analysis
  • Medicine
ispartof: European Journal of Cancer, February 2014, Vol.50(3), pp.628-637
description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.ejca.2013.11.015 Byline: Laurel T. Bate-Eya, Marli E. Ebus, Jan Koster, Ilona J.M. den Hartog, Danny A. Zwijnenburg, Linda Schild, Ida van der Ploeg, M. Emmy M. Dolman, Huib N. Caron, Rogier Versteeg, Jan J. Molenaar Abstract: Recently protocols have been devised for the culturing of cell lines from fresh tumours under serum-free conditions in defined neural stem cell medium. These cells, frequently called tumour initiating cells (TICs) closely retained characteristics of the tumours of origin. We report the isolation of eight newly-derived neuroblastoma TICs from six primary neuroblastoma tumours and two bone marrow metastases. The primary tumours from which these TICs were generated have previously been fully typed by whole genome sequencing (WGS). Array comparative genomic hybridisation (aCGH) analysis showed that TIC lines retained essential characteristics of the primary tumours and exhibited typical neuroblastoma chromosomal aberrations such as MYCN amplification, gain of chromosome 17q and deletion of 1p36. Protein analysis showed expression for neuroblastoma markers MYCN, NCAM, CHGA, DBH and TH while haematopoietic markers CD19 and CD11b were absent. We analysed the growth characteristics and confirmed tumour-forming potential using sphere-forming assays, subcutaneous and orthotopic injection of these cells into immune-compromised mice. Affymetrix mRNA expression profiling of TIC line xenografts showed an expression pattern more closely mimicking primary tumours compared to xenografts from classical cell lines. This establishes that these neuroblastoma TICs cultured under serum-free conditions are relevant and useful neuroblastoma tumour models.
language: eng
source:
identifier: ISSN: 0959-8049 ; E-ISSN: 1879-0852 ; DOI: 10.1016/j.ejca.2013.11.015
fulltext: fulltext
issn:
  • 0959-8049
  • 09598049
  • 1879-0852
  • 18790852
url: Link


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titleNewly-derived neuroblastoma cell lines propagated in serum-free media recapitulate the genotype and phenotype of primary neuroblastoma tumours
creatorBate-Eya, Laurel T ; Ebus, Marli E ; Koster, Jan ; Den Hartog, Ilona J.M ; Zwijnenburg, Danny A ; Schild, Linda ; van Der Ploeg, Ida ; Dolman, M. Emmy M ; Caron, Huib N ; Versteeg, Rogier ; Molenaar, Jan J
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titleNewly-derived neuroblastoma cell lines propagated in serum-free media recapitulate the genotype and phenotype of primary neuroblastoma tumours
description

Recently protocols have been devised for the culturing of cell lines from fresh tumours under serum-free conditions in defined neural stem cell medium. These cells, frequently called tumour initiating cells (TICs) closely retained characteristics of the tumours of origin. We report the isolation of eight newly-derived neuroblastoma TICs from six primary neuroblastoma tumours and two bone marrow metastases. The primary tumours from which these TICs were generated have previously been fully typed by whole genome sequencing (WGS). Array comparative genomic hybridisation (aCGH) analysis showed that TIC lines retained essential characteristics of the primary tumours and exhibited typical neuroblastoma chromosomal aberrations such as MYCN amplification, gain of chromosome 17q and deletion of 1p36. Protein analysis showed expression for neuroblastoma markers MYCN, NCAM, CHGA, DBH and TH while haematopoietic markers CD19 and CD11b were absent. We analysed the growth characteristics and...

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abstract

Recently protocols have been devised for the culturing of cell lines from fresh tumours under serum-free conditions in defined neural stem cell medium. These cells, frequently called tumour initiating cells (TICs) closely retained characteristics of the tumours of origin. We report the isolation of eight newly-derived neuroblastoma TICs from six primary neuroblastoma tumours and two bone marrow metastases. The primary tumours from which these TICs were generated have previously been fully typed by whole genome sequencing (WGS). Array comparative genomic hybridisation (aCGH) analysis showed that TIC lines retained essential characteristics of the primary tumours and exhibited typical neuroblastoma chromosomal aberrations such as MYCN amplification, gain of chromosome 17q and deletion of 1p36. Protein analysis showed expression for neuroblastoma markers MYCN, NCAM, CHGA, DBH and TH while haematopoietic markers CD19 and CD11b were absent. We analysed the growth characteristics and...

pubElsevier Ltd
doi10.1016/j.ejca.2013.11.015
lad01European Journal of Cancer
date2014-02