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Amniotic membrane extract ameliorates benzalkonium chloride-induced dry eye in a murine model

Human amniotic membrane (AM) is avascular but contains various beneficial bioactive factors, its extract (AE) is also effective in treating many ocular surface disorders. In this study, we for the first time evaluated the therapeutic effects of AE on dry eye induced by benzalkonium chloride in a BAL... Full description

Journal Title: Experimental Eye Research October 2013, Vol.115, pp.31-40
Main Author: Xiao, Xinye
Other Authors: Luo, Pingping , Zhao, Hui , Chen, Jingyao , He, Hui , Xu, Yuxue , Lin, Zhirong , Zhou, Yueping , Xu, Jianjiang , Liu, Zuguo
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0014-4835 ; E-ISSN: 1096-0007 ; DOI: 10.1016/j.exer.2013.06.005
Link: https://www.sciencedirect.com/science/article/pii/S0014483513001450
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recordid: elsevier_sdoi_10_1016_j_exer_2013_06_005
title: Amniotic membrane extract ameliorates benzalkonium chloride-induced dry eye in a murine model
format: Article
creator:
  • Xiao, Xinye
  • Luo, Pingping
  • Zhao, Hui
  • Chen, Jingyao
  • He, Hui
  • Xu, Yuxue
  • Lin, Zhirong
  • Zhou, Yueping
  • Xu, Jianjiang
  • Liu, Zuguo
subjects:
  • Dry Eye
  • Amniotic Membrane Extract
  • Benzalkonium Chloride
  • Anti-Inflammation
  • Pathological Change
  • Medicine
  • Anatomy & Physiology
ispartof: Experimental Eye Research, October 2013, Vol.115, pp.31-40
description: Human amniotic membrane (AM) is avascular but contains various beneficial bioactive factors, its extract (AE) is also effective in treating many ocular surface disorders. In this study, we for the first time evaluated the therapeutic effects of AE on dry eye induced by benzalkonium chloride in a BALB/c mouse model. Topical application of AE (1.5 and 3 μg/eye/day) resulted in significantly longer tear break-up time on Day 3 and 6, lower fluorescein staining scores on Day 3, and lower inflammatory index on Day 6. AE reduced corneal epithelial K10 expression, inflammatory infiltration, and levels of TNF-α, IL-1β and IL-6 in BAC treated mice than that in the control mice. Moreover, decreased TUNEL positive cells in cornea and increased goblet cells in conjunctiva were also observed in AE treated corneas. Finally, AE induced more Ki-67 positive cells in corneal epithelium of dry eye mouse. Taken together, our data provide further support for BAC induced dry eye model as a valuable for dry eye study and suggest a great potential for AE as a therapeutic agent in the clinical treatment of dry eye.
language: eng
source:
identifier: ISSN: 0014-4835 ; E-ISSN: 1096-0007 ; DOI: 10.1016/j.exer.2013.06.005
fulltext: fulltext
issn:
  • 0014-4835
  • 00144835
  • 1096-0007
  • 10960007
url: Link


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titleAmniotic membrane extract ameliorates benzalkonium chloride-induced dry eye in a murine model
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subjectDry Eye ; Amniotic Membrane Extract ; Benzalkonium Chloride ; Anti-Inflammation ; Pathological Change ; Medicine ; Anatomy & Physiology
descriptionHuman amniotic membrane (AM) is avascular but contains various beneficial bioactive factors, its extract (AE) is also effective in treating many ocular surface disorders. In this study, we for the first time evaluated the therapeutic effects of AE on dry eye induced by benzalkonium chloride in a BALB/c mouse model. Topical application of AE (1.5 and 3 μg/eye/day) resulted in significantly longer tear break-up time on Day 3 and 6, lower fluorescein staining scores on Day 3, and lower inflammatory index on Day 6. AE reduced corneal epithelial K10 expression, inflammatory infiltration, and levels of TNF-α, IL-1β and IL-6 in BAC treated mice than that in the control mice. Moreover, decreased TUNEL positive cells in cornea and increased goblet cells in conjunctiva were also observed in AE treated corneas. Finally, AE induced more Ki-67 positive cells in corneal epithelium of dry eye mouse. Taken together, our data provide further support for BAC induced dry eye model as a valuable for dry eye study and suggest a great potential for AE as a therapeutic agent in the clinical treatment of dry eye.
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titleAmniotic membrane extract ameliorates benzalkonium chloride-induced dry eye in a murine model
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Human amniotic membrane (AM) is avascular but contains various beneficial bioactive factors, its extract (AE) is also effective in treating many ocular surface disorders. In this study, we for the first time evaluated the therapeutic effects of AE on dry eye induced by benzalkonium chloride in a BALB/c mouse model. Topical application of AE (1.5 and 3 μg/eye/day) resulted in significantly longer tear break-up time on Day 3 and 6, lower fluorescein staining scores on Day 3, and lower inflammatory index on Day 6. AE reduced corneal epithelial K10 expression, inflammatory infiltration, and levels of TNF-α, IL-1β and IL-6 in BAC treated mice than that in the control mice. Moreover, decreased TUNEL positive cells in cornea and increased goblet cells in conjunctiva were also observed in AE treated corneas. Finally, AE induced more Ki-67 positive cells in corneal epithelium of dry eye mouse. Taken together, our data provide further support for BAC induced dry eye model as a valuable for dry eye study and suggest a great potential for AE as a therapeutic agent in the clinical treatment of dry eye.

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Human amniotic membrane (AM) is avascular but contains various beneficial bioactive factors, its extract (AE) is also effective in treating many ocular surface disorders. In this study, we for the first time evaluated the therapeutic effects of AE on dry eye induced by benzalkonium chloride in a BALB/c mouse model. Topical application of AE (1.5 and 3 μg/eye/day) resulted in significantly longer tear break-up time on Day 3 and 6, lower fluorescein staining scores on Day 3, and lower inflammatory index on Day 6. AE reduced corneal epithelial K10 expression, inflammatory infiltration, and levels of TNF-α, IL-1β and IL-6 in BAC treated mice than that in the control mice. Moreover, decreased TUNEL positive cells in cornea and increased goblet cells in conjunctiva were also observed in AE treated corneas. Finally, AE induced more Ki-67 positive cells in corneal epithelium of dry eye mouse. Taken together, our data provide further support for BAC induced dry eye model as a valuable for dry eye study and suggest a great potential for AE as a therapeutic agent in the clinical treatment of dry eye.

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lad01Experimental Eye Research
date2013-10