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Transduced PEP-1-FK506BP inhibits the inflammatory response in the Raw 264.7 cell and mouse models

FK506 binding protein 12 (FK506BP) is an immunophilin that acts as a receptor for the immunosuppressant drug FK506. Although the precise action of FK506BP remains unclear, it has emerged as a potential drug target for several inflammatory diseases. This study investigated the protective effects of F... Full description

Journal Title: Immunobiology 2011, Vol.216(7), pp.771-781
Main Author: Kim, So Young
Other Authors: Jeong, Hoon Jae , Kim, Dae Won , Kim, Mi Jin , An, Jae Jin , Sohn, Eun Jeong , Kang, Hye Won , Shin, Min Jea , Ahn, Eun Hee , Kwon, Soon Won , Kim, Duk-Soo , Cho, Sung-Woo , Park, Jinseu , Eum, Won Sik , Choi, Soo Young
Format: Electronic Article Electronic Article
Language: English
Subjects:
Lps
Tpa
ID: ISSN: 0171-2985 ; E-ISSN: 1878-3279 ; DOI: 10.1016/j.imbio.2010.12.008
Link: http://dx.doi.org/10.1016/j.imbio.2010.12.008
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recordid: elsevier_sdoi_10_1016_j_imbio_2010_12_008
title: Transduced PEP-1-FK506BP inhibits the inflammatory response in the Raw 264.7 cell and mouse models
format: Article
creator:
  • Kim, So Young
  • Jeong, Hoon Jae
  • Kim, Dae Won
  • Kim, Mi Jin
  • An, Jae Jin
  • Sohn, Eun Jeong
  • Kang, Hye Won
  • Shin, Min Jea
  • Ahn, Eun Hee
  • Kwon, Soon Won
  • Kim, Duk-Soo
  • Cho, Sung-Woo
  • Park, Jinseu
  • Eum, Won Sik
  • Choi, Soo Young
subjects:
  • Cytokines
  • Inflammation
  • Mitogen-Activated Protein Kinase
  • Nf-Κb
  • PEP-1-Fk506bp
  • Protein Therapy
  • Cox-2
  • Fk506bp
  • Lps
  • Mapk
  • Nf-Κb
  • Tpa
  • Cytokines
  • Inflammation
  • Mitogen-Activated Protein Kinase
  • Nf-Κb
  • PEP-1-Fk506bp
ispartof: Immunobiology, 2011, Vol.216(7), pp.771-781
description: FK506 binding protein 12 (FK506BP) is an immunophilin that acts as a receptor for the immunosuppressant drug FK506. Although the precise action of FK506BP remains unclear, it has emerged as a potential drug target for several inflammatory diseases. This study investigated the protective effects of FK506BP on inflammation in vitro and in vivo using protein transduction. A cell-permeable expression vector PEP-1-FK506BP was constructed. Lipopolysaccharide (LPS)- or 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated Raw 264.7 cells and ICR mice were treated with PEP-1-FK506BP. The expression of inflammatory response enzymes and cytokines was analyzed by Western blot, reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, and electrophoretic mobility shift assay. PEP-1-FK506BP efficiently transduced into Raw 264.7 cells and markedly inhibited the expression levels of cyclooxygenase-2 as well as pro-inflammatory cytokines. Furthermore, transduced...
language: eng
source:
identifier: ISSN: 0171-2985 ; E-ISSN: 1878-3279 ; DOI: 10.1016/j.imbio.2010.12.008
fulltext: fulltext
issn:
  • 0171-2985
  • 01712985
  • 1878-3279
  • 18783279
url: Link


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titleTransduced PEP-1-FK506BP inhibits the inflammatory response in the Raw 264.7 cell and mouse models
creatorKim, So Young ; Jeong, Hoon Jae ; Kim, Dae Won ; Kim, Mi Jin ; An, Jae Jin ; Sohn, Eun Jeong ; Kang, Hye Won ; Shin, Min Jea ; Ahn, Eun Hee ; Kwon, Soon Won ; Kim, Duk-Soo ; Cho, Sung-Woo ; Park, Jinseu ; Eum, Won Sik ; Choi, Soo Young
ispartofImmunobiology, 2011, Vol.216(7), pp.771-781
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descriptionFK506 binding protein 12 (FK506BP) is an immunophilin that acts as a receptor for the immunosuppressant drug FK506. Although the precise action of FK506BP remains unclear, it has emerged as a potential drug target for several inflammatory diseases. This study investigated the protective effects of FK506BP on inflammation in vitro and in vivo using protein transduction. A cell-permeable expression vector PEP-1-FK506BP was constructed. Lipopolysaccharide (LPS)- or 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated Raw 264.7 cells and ICR mice were treated with PEP-1-FK506BP. The expression of inflammatory response enzymes and cytokines was analyzed by Western blot, reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, and electrophoretic mobility shift assay. PEP-1-FK506BP efficiently transduced into Raw 264.7 cells and markedly inhibited the expression levels of cyclooxygenase-2 as well as pro-inflammatory cytokines. Furthermore, transduced...
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FK506 binding protein 12 (FK506BP) is an immunophilin that acts as a receptor for the immunosuppressant drug FK506. Although the precise action of FK506BP remains unclear, it has emerged as a potential drug target for several inflammatory diseases. This study investigated the protective effects of FK506BP on inflammation in vitro and in vivo using protein transduction. A cell-permeable expression vector PEP-1-FK506BP was constructed. Lipopolysaccharide (LPS)- or 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated Raw 264.7 cells and ICR mice were treated with PEP-1-FK506BP. The expression of inflammatory response enzymes and cytokines was analyzed by Western blot, reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, and electrophoretic mobility shift assay. PEP-1-FK506BP efficiently transduced into Raw 264.7 cells and markedly inhibited the expression levels of cyclooxygenase-2 as well as pro-inflammatory cytokines. Furthermore, transduced...

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atitleTransduced PEP-1-FK506BP inhibits the inflammatory response in the Raw 264.7 cell and mouse models
jtitleImmunobiology
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abstract

FK506 binding protein 12 (FK506BP) is an immunophilin that acts as a receptor for the immunosuppressant drug FK506. Although the precise action of FK506BP remains unclear, it has emerged as a potential drug target for several inflammatory diseases. This study investigated the protective effects of FK506BP on inflammation in vitro and in vivo using protein transduction. A cell-permeable expression vector PEP-1-FK506BP was constructed. Lipopolysaccharide (LPS)- or 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated Raw 264.7 cells and ICR mice were treated with PEP-1-FK506BP. The expression of inflammatory response enzymes and cytokines was analyzed by Western blot, reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, and electrophoretic mobility shift assay. PEP-1-FK506BP efficiently transduced into Raw 264.7 cells and markedly inhibited the expression levels of cyclooxygenase-2 as well as pro-inflammatory cytokines. Furthermore, transduced...

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