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10-Hydroxy-2-decenoic acid inhibiting the proliferation of fibroblast-like synoviocytes by PI3K–AKT pathway

To reveal the mechanism of 10H2DA inhibiting the proliferation of fibroblast-like synoviocytes (FLSs) of RA patients. Cell proliferation, HDAC activity and histone acetylation level of FLS cells treated with 10H2DA were detected by MTT assay, Colorimetric HDAC Activity Assay and Western-blot. Differ... Full description

Journal Title: International Immunopharmacology September 2015, Vol.28(1), pp.97-104
Main Author: Wang, Jianguang
Other Authors: Zhang, Wei , Zou, Hai , Lin, Yaoyao , Lin, Ke , Zhou, Zhonghao , Qiang, Jiaping , Lin, Jialiang , Chuka, Chifundo Martha , Ge, Renshan , Zhao, Shujiang , Yang, Xinyu
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1567-5769 ; E-ISSN: 1878-1705 ; DOI: 10.1016/j.intimp.2015.05.036
Link: https://www.sciencedirect.com/science/article/pii/S1567576915002659
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recordid: elsevier_sdoi_10_1016_j_intimp_2015_05_036
title: 10-Hydroxy-2-decenoic acid inhibiting the proliferation of fibroblast-like synoviocytes by PI3K–AKT pathway
format: Article
creator:
  • Wang, Jianguang
  • Zhang, Wei
  • Zou, Hai
  • Lin, Yaoyao
  • Lin, Ke
  • Zhou, Zhonghao
  • Qiang, Jiaping
  • Lin, Jialiang
  • Chuka, Chifundo Martha
  • Ge, Renshan
  • Zhao, Shujiang
  • Yang, Xinyu
subjects:
  • Rheumatoid Arthritis
  • 10h2da
  • Hdacis
  • Microarray
  • Pi3k–Akt Pathway
  • Biology
  • Pharmacy, Therapeutics, & Pharmacology
ispartof: International Immunopharmacology, September 2015, Vol.28(1), pp.97-104
description: To reveal the mechanism of 10H2DA inhibiting the proliferation of fibroblast-like synoviocytes (FLSs) of RA patients. Cell proliferation, HDAC activity and histone acetylation level of FLS cells treated with 10H2DA were detected by MTT assay, Colorimetric HDAC Activity Assay and Western-blot. Different genes in FLS cells from RA patients were primary cultured and treated with 10H2DA. They were then screened by Human Transcriptome 1.0 ST microarrays and verified by real-time PCR. The results showed dose-dependent and time-dependent decreases in cell viability and HDAC activity in FLSs treated with 10H2DA, and time-dependent induction in the acetylation of H3 and H4 at the same time. 697 different genes were identified by HTA 1.0. The expressions of 7 target genes of the PI3K–AKT pathway were decreased and 4 target genes of cytokine-cytokine receptor interaction were increased verified by real-time PCR. These results imply that 10H2DA is a potential HDACI which inhibits the proliferation of FLS cells by PI3K–AKT pathway.
language: eng
source:
identifier: ISSN: 1567-5769 ; E-ISSN: 1878-1705 ; DOI: 10.1016/j.intimp.2015.05.036
fulltext: fulltext
issn:
  • 1567-5769
  • 15675769
  • 1878-1705
  • 18781705
url: Link


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title10-Hydroxy-2-decenoic acid inhibiting the proliferation of fibroblast-like synoviocytes by PI3K–AKT pathway
creatorWang, Jianguang ; Zhang, Wei ; Zou, Hai ; Lin, Yaoyao ; Lin, Ke ; Zhou, Zhonghao ; Qiang, Jiaping ; Lin, Jialiang ; Chuka, Chifundo Martha ; Ge, Renshan ; Zhao, Shujiang ; Yang, Xinyu
ispartofInternational Immunopharmacology, September 2015, Vol.28(1), pp.97-104
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subjectRheumatoid Arthritis ; 10h2da ; Hdacis ; Microarray ; Pi3k–Akt Pathway ; Biology ; Pharmacy, Therapeutics, & Pharmacology
descriptionTo reveal the mechanism of 10H2DA inhibiting the proliferation of fibroblast-like synoviocytes (FLSs) of RA patients. Cell proliferation, HDAC activity and histone acetylation level of FLS cells treated with 10H2DA were detected by MTT assay, Colorimetric HDAC Activity Assay and Western-blot. Different genes in FLS cells from RA patients were primary cultured and treated with 10H2DA. They were then screened by Human Transcriptome 1.0 ST microarrays and verified by real-time PCR. The results showed dose-dependent and time-dependent decreases in cell viability and HDAC activity in FLSs treated with 10H2DA, and time-dependent induction in the acetylation of H3 and H4 at the same time. 697 different genes were identified by HTA 1.0. The expressions of 7 target genes of the PI3K–AKT pathway were decreased and 4 target genes of cytokine-cytokine receptor interaction were increased verified by real-time PCR. These results imply that 10H2DA is a potential HDACI which inhibits the proliferation of FLS cells by PI3K–AKT pathway.
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title10-Hydroxy-2-decenoic acid inhibiting the proliferation of fibroblast-like synoviocytes by PI3K–AKT pathway
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To reveal the mechanism of 10H2DA inhibiting the proliferation of fibroblast-like synoviocytes (FLSs) of RA patients. Cell proliferation, HDAC activity and histone acetylation level of FLS cells treated with 10H2DA were detected by MTT assay, Colorimetric HDAC Activity Assay and Western-blot. Different genes in FLS cells from RA patients were primary cultured and treated with 10H2DA. They were then screened by Human Transcriptome 1.0 ST microarrays and verified by real-time PCR. The results showed dose-dependent and time-dependent decreases in cell viability and HDAC activity in FLSs treated with 10H2DA, and time-dependent induction in the acetylation of H3 and H4 at the same time. 697 different genes were identified by HTA 1.0. The expressions of 7 target genes of the PI3K–AKT pathway were decreased and 4 target genes of cytokine-cytokine receptor interaction were increased verified by real-time PCR. These results imply that 10H2DA is a potential HDACI which inhibits the proliferation of FLS cells by PI3K–AKT pathway.

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To reveal the mechanism of 10H2DA inhibiting the proliferation of fibroblast-like synoviocytes (FLSs) of RA patients. Cell proliferation, HDAC activity and histone acetylation level of FLS cells treated with 10H2DA were detected by MTT assay, Colorimetric HDAC Activity Assay and Western-blot. Different genes in FLS cells from RA patients were primary cultured and treated with 10H2DA. They were then screened by Human Transcriptome 1.0 ST microarrays and verified by real-time PCR. The results showed dose-dependent and time-dependent decreases in cell viability and HDAC activity in FLSs treated with 10H2DA, and time-dependent induction in the acetylation of H3 and H4 at the same time. 697 different genes were identified by HTA 1.0. The expressions of 7 target genes of the PI3K–AKT pathway were decreased and 4 target genes of cytokine-cytokine receptor interaction were increased verified by real-time PCR. These results imply that 10H2DA is a potential HDACI which inhibits the proliferation of FLS cells by PI3K–AKT pathway.

pubElsevier B.V
doi10.1016/j.intimp.2015.05.036
lad01International Immunopharmacology
date2015-09