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Expression and Functional Role of Cdx2 in Intestinal Metaplasia of Cystitis Glandularis

Cdx2 is an essential transcription factor in intestinal epithelial cell differentiation and proliferation. However, to our knowledge the expression and role of Cdx2 in the development of intestinal cystitis glandularis, a metaplastic lesion induced by chronic inflammation, remained to be explored. R... Full description

Journal Title: The Journal of Urology September 2013, Vol.190(3), pp.1083-1089
Main Author: Lin, Ming-En
Other Authors: Huang, Danying , Deng, Bi-Hua , Lv, Yi-Song , Rong, Lu , Yao, You-Sheng
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0022-5347 ; E-ISSN: 1527-3792 ; DOI: 10.1016/j.juro.2013.03.109
Link: https://www.sciencedirect.com/science/article/pii/S002253471303961X
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recordid: elsevier_sdoi_10_1016_j_juro_2013_03_109
title: Expression and Functional Role of Cdx2 in Intestinal Metaplasia of Cystitis Glandularis
format: Article
creator:
  • Lin, Ming-En
  • Huang, Danying
  • Deng, Bi-Hua
  • Lv, Yi-Song
  • Rong, Lu
  • Yao, You-Sheng
subjects:
  • Urinary Bladder
  • Cystitis
  • Cdx2 Protein, Human
  • Villin
  • Cdh17 Protein, Human
  • Medicine
ispartof: The Journal of Urology, September 2013, Vol.190(3), pp.1083-1089
description: Cdx2 is an essential transcription factor in intestinal epithelial cell differentiation and proliferation. However, to our knowledge the expression and role of Cdx2 in the development of intestinal cystitis glandularis, a metaplastic lesion induced by chronic inflammation, remained to be explored. Real-time polymerase chain reaction was used to examine Cdx2, LI-cadherin and villin expression in typical and intestinal cystitis glandularis, and normal bladder tissue. Cdx2 cDNA was subcloned to the retroviral vector pLNCX2 for subsequent transfection into human bladder urothelium cells and rat bladder urothelium. Cdx2 mRNA and protein levels, and cell morphology and proliferation were assessed after transfection using real-time polymerase chain reaction, phase contrast microscopy, transmission electron microscopy and MTT assay, respectively. Higher mRNA levels of Cdx2, villin and LI-cadherin were detected in intestinal cystitis glandularis compared to normal bladder and typical cystitis glandularis. Only Cdx2 groups attained statistical significance (p
language: eng
source:
identifier: ISSN: 0022-5347 ; E-ISSN: 1527-3792 ; DOI: 10.1016/j.juro.2013.03.109
fulltext: fulltext
issn:
  • 0022-5347
  • 00225347
  • 1527-3792
  • 15273792
url: Link


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titleExpression and Functional Role of Cdx2 in Intestinal Metaplasia of Cystitis Glandularis
creatorLin, Ming-En ; Huang, Danying ; Deng, Bi-Hua ; Lv, Yi-Song ; Rong, Lu ; Yao, You-Sheng
ispartofThe Journal of Urology, September 2013, Vol.190(3), pp.1083-1089
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subjectUrinary Bladder ; Cystitis ; Cdx2 Protein, Human ; Villin ; Cdh17 Protein, Human ; Medicine
descriptionCdx2 is an essential transcription factor in intestinal epithelial cell differentiation and proliferation. However, to our knowledge the expression and role of Cdx2 in the development of intestinal cystitis glandularis, a metaplastic lesion induced by chronic inflammation, remained to be explored. Real-time polymerase chain reaction was used to examine Cdx2, LI-cadherin and villin expression in typical and intestinal cystitis glandularis, and normal bladder tissue. Cdx2 cDNA was subcloned to the retroviral vector pLNCX2 for subsequent transfection into human bladder urothelium cells and rat bladder urothelium. Cdx2 mRNA and protein levels, and cell morphology and proliferation were assessed after transfection using real-time polymerase chain reaction, phase contrast microscopy, transmission electron microscopy and MTT assay, respectively. Higher mRNA levels of Cdx2, villin and LI-cadherin were detected in intestinal cystitis glandularis compared to normal bladder and typical cystitis glandularis. Only Cdx2 groups attained statistical significance (p <0.001). Retroviral over expression of Cdx2 resulted in increased mRNA and protein expression of Cdx2 as well as villin and LI-cadherin levels, and increased cell proliferation. A distinct change in cellular morphology, in which cells resembled intestinal-like cells, was also observed in vitro and in vivo. Cdx2 may have a critical role in regulating intestinal metaplasia in cystitis glandularis. Further studies are planned to assess the potential of using Cdx2 as a marker and therapeutic target for cystitis glandularis.
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