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Effect of methionine sulfoxide reductase B1 silencing on high-glucose-induced apoptosis of human lens epithelial cells

To determine roles of methionine sulfoxide reductase B1 (MsrB1) in protecting lens mitochondria against oxidative damage, the influences of MsrB1 gene silencing on high-glucose-induced apoptosis in human lens epithelial (HLE) cells were studied. Our study used four groups of cells: normal control, M... Full description

Journal Title: Life Sciences 27 February 2013, Vol.92(3), pp.193-201
Main Author: Li, Yi
Other Authors: Jia, Yi , Zhou, Jun , Huang, Kaixun
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0024-3205 ; E-ISSN: 1879-0631 ; DOI: 10.1016/j.lfs.2012.11.021
Link: https://www.sciencedirect.com/science/article/pii/S0024320512007163
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recordid: elsevier_sdoi_10_1016_j_lfs_2012_11_021
title: Effect of methionine sulfoxide reductase B1 silencing on high-glucose-induced apoptosis of human lens epithelial cells
format: Article
creator:
  • Li, Yi
  • Jia, Yi
  • Zhou, Jun
  • Huang, Kaixun
subjects:
  • Apoptosis
  • Cytochrome C
  • High Glucose
  • Human Lens Epithelial Cells
  • Methionine Sulfoxide Reductase B1
  • Mitochondrial Membrane Potential
  • Sciences (General)
  • Biology
ispartof: Life Sciences, 27 February 2013, Vol.92(3), pp.193-201
description: To determine roles of methionine sulfoxide reductase B1 (MsrB1) in protecting lens mitochondria against oxidative damage, the influences of MsrB1 gene silencing on high-glucose-induced apoptosis in human lens epithelial (HLE) cells were studied. Our study used four groups of cells: normal control, MsrB1 gene silenced, high glucose (30 mM) exposed and MsrB1 gene silenced cells followed with high glucose exposure. In all cases we detected cell viability, cell apoptosis rate, intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels, alteration of mitochondrial membrane potential, release of mitochondrial cytochrome c as well as an increase in activity of caspase-3. The results showed that MsrB1 gene silencing by short interfering RNA (siRNA) in HLE cells clearly resulted in oxidative stress, decrease in mitochondrial membrane potential and release of mitochondrial cytochrome c as well as an increase in activity...
language: eng
source:
identifier: ISSN: 0024-3205 ; E-ISSN: 1879-0631 ; DOI: 10.1016/j.lfs.2012.11.021
fulltext: fulltext
issn:
  • 0024-3205
  • 00243205
  • 1879-0631
  • 18790631
url: Link


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titleEffect of methionine sulfoxide reductase B1 silencing on high-glucose-induced apoptosis of human lens epithelial cells
creatorLi, Yi ; Jia, Yi ; Zhou, Jun ; Huang, Kaixun
ispartofLife Sciences, 27 February 2013, Vol.92(3), pp.193-201
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subjectApoptosis ; Cytochrome C ; High Glucose ; Human Lens Epithelial Cells ; Methionine Sulfoxide Reductase B1 ; Mitochondrial Membrane Potential ; Sciences (General) ; Biology
descriptionTo determine roles of methionine sulfoxide reductase B1 (MsrB1) in protecting lens mitochondria against oxidative damage, the influences of MsrB1 gene silencing on high-glucose-induced apoptosis in human lens epithelial (HLE) cells were studied. Our study used four groups of cells: normal control, MsrB1 gene silenced, high glucose (30 mM) exposed and MsrB1 gene silenced cells followed with high glucose exposure. In all cases we detected cell viability, cell apoptosis rate, intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels, alteration of mitochondrial membrane potential, release of mitochondrial cytochrome c as well as an increase in activity of caspase-3. The results showed that MsrB1 gene silencing by short interfering RNA (siRNA) in HLE cells clearly resulted in oxidative stress, decrease in mitochondrial membrane potential and release of mitochondrial cytochrome c as well as an increase in activity...
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