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Comparative structural and functional studies of nanoparticle formulations for DNA and siRNA delivery

The transfection efficiencies of 25-kDa branched polyethylenimine (B-PEI) and 22-kDa linear PEI (L-PEI) with both DNA and small interfering RNA (siRNA) were compared and correlated with their biophysical properties relating to complex formation, stability, and disassembly. L-PEI-DNA complexes transf... Full description

Journal Title: Nanomedicine: Nanotechnology Biology, and Medicine, 2011, Vol.7(2), pp.210-219
Main Author: Kwok, Albert
Other Authors: Hart, Stephen L
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1549-9634 ; E-ISSN: 1549-9642 ; DOI: 10.1016/j.nano.2010.07.005
Link: http://dx.doi.org/10.1016/j.nano.2010.07.005
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recordid: elsevier_sdoi_10_1016_j_nano_2010_07_005
title: Comparative structural and functional studies of nanoparticle formulations for DNA and siRNA delivery
format: Article
creator:
  • Kwok, Albert
  • Hart, Stephen L
subjects:
  • Polyethylenimine (PEI)
  • Biophysical Characteristics
  • DNA Delivery
  • Sirna Delivery
  • RNA Interference
  • Polyethylenimine (PEI)
  • Biophysical Characteristics
  • DNA Delivery
  • Sirna Delivery
  • RNA Interference
  • Medicine
ispartof: Nanomedicine: Nanotechnology, Biology, and Medicine, 2011, Vol.7(2), pp.210-219
description: The transfection efficiencies of 25-kDa branched polyethylenimine (B-PEI) and 22-kDa linear PEI (L-PEI) with both DNA and small interfering RNA (siRNA) were compared and correlated with their biophysical properties relating to complex formation, stability, and disassembly. L-PEI-DNA complexes transfected (5.18 × 10 8 relative luminescence units [RLU]/mg) around fivefold better than B-PEI-DNA complexes (0.95 × 10 8 RLU/mg), whereas B-PEI-siRNA complexes gave approximately 60% gene knockdown and L-PEI-siRNA complexes were inactive. Both B-PEI and L-PEI packaged DNA and siRNA to form positively charged nanoparticles; however, L-PEI nanoparticles were less stable than B-PEI nanoparticles, particularly with siRNA. The poor stability of L-PEI-siRNA complexes seemed to be the major factor contributing to an observed lack of cellular uptake and hence poor transfection. The more stable B-PEI-siRNA complexes, however, were bound, internalized, and detectable in the cytoplasm. These...
language: eng
source:
identifier: ISSN: 1549-9634 ; E-ISSN: 1549-9642 ; DOI: 10.1016/j.nano.2010.07.005
fulltext: fulltext
issn:
  • 1549-9634
  • 15499634
  • 1549-9642
  • 15499642
url: Link


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descriptionThe transfection efficiencies of 25-kDa branched polyethylenimine (B-PEI) and 22-kDa linear PEI (L-PEI) with both DNA and small interfering RNA (siRNA) were compared and correlated with their biophysical properties relating to complex formation, stability, and disassembly. L-PEI-DNA complexes transfected (5.18 × 10 8 relative luminescence units [RLU]/mg) around fivefold better than B-PEI-DNA complexes (0.95 × 10 8 RLU/mg), whereas B-PEI-siRNA complexes gave approximately 60% gene knockdown and L-PEI-siRNA complexes were inactive. Both B-PEI and L-PEI packaged DNA and siRNA to form positively charged nanoparticles; however, L-PEI nanoparticles were less stable than B-PEI nanoparticles, particularly with siRNA. The poor stability of L-PEI-siRNA complexes seemed to be the major factor contributing to an observed lack of cellular uptake and hence poor transfection. The more stable B-PEI-siRNA complexes, however, were bound, internalized, and detectable in the cytoplasm. These...
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The transfection efficiencies of 25-kDa branched polyethylenimine (B-PEI) and 22-kDa linear PEI (L-PEI) with both DNA and small interfering RNA (siRNA) were compared and correlated with their biophysical properties relating to complex formation, stability, and disassembly. L-PEI-DNA complexes transfected (5.18 × 10 8 relative luminescence units [RLU]/mg) around fivefold better than B-PEI-DNA complexes (0.95 × 10 8 RLU/mg), whereas B-PEI-siRNA complexes gave approximately 60% gene knockdown and L-PEI-siRNA complexes were inactive. Both B-PEI and L-PEI packaged DNA and siRNA to form positively charged nanoparticles; however, L-PEI nanoparticles were less stable than B-PEI nanoparticles, particularly with siRNA. The poor stability of L-PEI-siRNA complexes seemed to be the major factor contributing to an observed lack of cellular uptake and hence poor transfection. The more stable B-PEI-siRNA complexes, however, were bound, internalized, and detectable in the cytoplasm. These...

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