schliessen

Filtern

 

Bibliotheken

Designing the epitope flanking regions for optimal generation of CTL epitopes

The flanking amino acids that surround epitopes are critical for effective antigen processing and maintenance of epitope integrity. In the present study, the frequency and characteristics of each amino acid that flanked the peptides generated from the proteasomal degradation of three different subty... Full description

Journal Title: Vaccine 12 June 2014, Vol.32(28), pp.3509-3516
Main Author: Steers, Nicholas J
Other Authors: Currier, Jeffrey R , Jobe, Ousman , Tovanabutra, Sodsai , Ratto-Kim, Silvia , Marovich, Mary A , Kim, Jerome H , Michael, Nelson L , Alving, Carl R , Rao, Mangala
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0264-410X ; E-ISSN: 1873-2518 ; DOI: 10.1016/j.vaccine.2014.04.039
Link: https://www.sciencedirect.com/science/article/pii/S0264410X14005775
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: elsevier_sdoi_10_1016_j_vaccine_2014_04_039
title: Designing the epitope flanking regions for optimal generation of CTL epitopes
format: Article
creator:
  • Steers, Nicholas J
  • Currier, Jeffrey R
  • Jobe, Ousman
  • Tovanabutra, Sodsai
  • Ratto-Kim, Silvia
  • Marovich, Mary A
  • Kim, Jerome H
  • Michael, Nelson L
  • Alving, Carl R
  • Rao, Mangala
subjects:
  • HIV-1
  • Gag-P24
  • Proteasome
  • Ctl-Epitope
  • Synthetic Flanking Region
  • Immunogen Design
  • Medicine
  • Biology
  • Veterinary Medicine
  • Pharmacy, Therapeutics, & Pharmacology
ispartof: Vaccine, 12 June 2014, Vol.32(28), pp.3509-3516
description: The flanking amino acids that surround epitopes are critical for effective antigen processing and maintenance of epitope integrity. In the present study, the frequency and characteristics of each amino acid that flanked the peptides generated from the proteasomal degradation of three different subtypes of HIV-1 Gag-p24 were determined. Synthetic flanking regions were designed based on the highest and the lowest frequencies of amino acid with the ideal characteristics at positions upstream and downstream of the proteasomal cleavage site. Peptides were synthesized that contained known CD8+ CTL-epitopes from HIV-1 Gag, CMV pp65, and vaccinia proteins HRP-2, and C16, flanked by amino acid sequences specifically designed to either generate or inhibit the known CD8+ CTL-epitopes. As predicted, the known CD8+ CTL-epitopes were effectively generated from the peptides with synthetic flanking regions specifically designed to promote epitope generation in a proteasome-dependent manner. The...
language: eng
source:
identifier: ISSN: 0264-410X ; E-ISSN: 1873-2518 ; DOI: 10.1016/j.vaccine.2014.04.039
fulltext: fulltext
issn:
  • 0264-410X
  • 0264410X
  • 1873-2518
  • 18732518
url: Link


@attributes
ID1635082160
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordiddoi_10_1016_j_vaccine_2014_04_039
sourceidelsevier_s
recordidTN_elsevier_sdoi_10_1016_j_vaccine_2014_04_039
sourcesystemPC
dbid
0--K
1--M
2.FO
3.~1
41B1
51P~
61RT
71~.
8457
94G.
107-5
118P~
129JM
13AABNK
14AAEDT
15AAKOC
16AAOAW
17AAQFI
18AARKO
19ABBQC
20ABFNM
21ABKYH
22ABMZM
23ABRWV
24ABYKQ
25ACDAQ
26ACIUM
27ACRLP
28AEKER
29AEVXI
30AEXOQ
31AFKWA
32AFTJW
33AFXIZ
34AGEKW
35AGUBO
36AGYEJ
37AIKHN
38AITUG
39AJBFU
40AJOXV
41AJRQY
42AJUYK
43AMFUW
44ANZVX
45BLXMC
46BNPGV
47CJTIS
48EO8
49EO9
50EP2
51EP3
52FDB
53FIRID
54FNPLU
55G-Q
56GBLVA
57J1W
58KOM
59LCYCR
60LUGTX
61O9~
62OAUVE
63OK0
64P-8
65P-9
66PC.
67Q38
68QYZTP
69RPZ
70SCC
71SDF
72SDG
73SDP
74SES
75SNL
76SSH
77SSI
78SSZ
79T5K
80UV1
81Z5R
82~G-
pqid1527453886
galeid519555886
display
typearticle
titleDesigning the epitope flanking regions for optimal generation of CTL epitopes
creatorSteers, Nicholas J ; Currier, Jeffrey R ; Jobe, Ousman ; Tovanabutra, Sodsai ; Ratto-Kim, Silvia ; Marovich, Mary A ; Kim, Jerome H ; Michael, Nelson L ; Alving, Carl R ; Rao, Mangala
ispartofVaccine, 12 June 2014, Vol.32(28), pp.3509-3516
identifier
subjectHIV-1 ; Gag-P24 ; Proteasome ; Ctl-Epitope ; Synthetic Flanking Region ; Immunogen Design ; Medicine ; Biology ; Veterinary Medicine ; Pharmacy, Therapeutics, & Pharmacology
descriptionThe flanking amino acids that surround epitopes are critical for effective antigen processing and maintenance of epitope integrity. In the present study, the frequency and characteristics of each amino acid that flanked the peptides generated from the proteasomal degradation of three different subtypes of HIV-1 Gag-p24 were determined. Synthetic flanking regions were designed based on the highest and the lowest frequencies of amino acid with the ideal characteristics at positions upstream and downstream of the proteasomal cleavage site. Peptides were synthesized that contained known CD8+ CTL-epitopes from HIV-1 Gag, CMV pp65, and vaccinia proteins HRP-2, and C16, flanked by amino acid sequences specifically designed to either generate or inhibit the known CD8+ CTL-epitopes. As predicted, the known CD8+ CTL-epitopes were effectively generated from the peptides with synthetic flanking regions specifically designed to promote epitope generation in a proteasome-dependent manner. The...
languageeng
source
version7
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttps://www.sciencedirect.com/science/article/pii/S0264410X14005775$$EView_record_in_ScienceDirect_(Access_to_full_text_may_be_restricted)
search
creatorcontrib
0Steers, Nicholas J
1Currier, Jeffrey R
2Jobe, Ousman
3Tovanabutra, Sodsai
4Ratto-Kim, Silvia
5Marovich, Mary A
6Kim, Jerome H
7Michael, Nelson L
8Alving, Carl R
9Rao, Mangala
titleDesigning the epitope flanking regions for optimal generation of CTL epitopes
description

The flanking amino acids that surround epitopes are critical for effective antigen processing and maintenance of epitope integrity. In the present study, the frequency and characteristics of each amino acid that flanked the peptides generated from the proteasomal degradation of three different subtypes of HIV-1 Gag-p24 were determined. Synthetic flanking regions were designed based on the highest and the lowest frequencies of amino acid with the ideal characteristics at positions upstream and downstream of the proteasomal cleavage site. Peptides were synthesized that contained known CD8+ CTL-epitopes from HIV-1 Gag, CMV pp65, and vaccinia proteins HRP-2, and C16, flanked by amino acid sequences specifically designed to either generate or inhibit the known CD8+ CTL-epitopes. As predicted, the known CD8+ CTL-epitopes were effectively generated from the peptides with synthetic flanking regions specifically designed to promote epitope generation in a proteasome-dependent manner. The...

subject
0HIV-1
1Gag-P24
2Proteasome
3Ctl-Epitope
4Synthetic Flanking Region
5Immunogen Design
6Medicine
7Biology
8Veterinary Medicine
9Pharmacy, Therapeutics, & Pharmacology
general
0English
1Elsevier Ltd
210.1016/j.vaccine.2014.04.039
3ScienceDirect (Elsevier)
4ScienceDirect Journals (Elsevier)
sourceidelsevier_s
recordidelsevier_sdoi_10_1016_j_vaccine_2014_04_039
issn
00264-410X
10264410X
21873-2518
318732518
rsrctypearticle
creationdate2014
addtitleVaccine
searchscope
0elsevier_full
1elsevier2
scope
0elsevier_full
1elsevier2
lsr44$$EView_record_in_ScienceDirect_(Access_to_full_text_may_be_restricted)
tmp01ScienceDirect Journals (Elsevier)
tmp02
0--K
1--M
2.FO
3.~1
41B1
51P~
61RT
71~.
8457
94G.
107-5
118P~
129JM
13AABNK
14AAEDT
15AAKOC
16AAOAW
17AAQFI
18AARKO
19ABBQC
20ABFNM
21ABKYH
22ABMZM
23ABRWV
24ABYKQ
25ACDAQ
26ACIUM
27ACRLP
28AEKER
29AEVXI
30AEXOQ
31AFKWA
32AFTJW
33AFXIZ
34AGEKW
35AGUBO
36AGYEJ
37AIKHN
38AITUG
39AJBFU
40AJOXV
41AJRQY
42AJUYK
43AMFUW
44ANZVX
45BLXMC
46BNPGV
47CJTIS
48EO8
49EO9
50EP2
51EP3
52FDB
53FIRID
54FNPLU
55G-Q
56GBLVA
57J1W
58KOM
59LCYCR
60LUGTX
61O9~
62OAUVE
63OK0
64P-8
65P-9
66PC.
67Q38
68QYZTP
69RPZ
70SCC
71SDF
72SDG
73SDP
74SES
75SNL
76SSH
77SSI
78SSZ
79T5K
80UV1
81Z5R
82~G-
startdate20140612
enddate20140612
lsr40Vaccine, 12 June 2014, Vol.32 (28), pp.3509-3516
doi10.1016/j.vaccine.2014.04.039
citationpf 3509 pt 3516 vol 32 issue 28
lsr30VSR-Enriched:[pqid, galeid]
sort
titleDesigning the epitope flanking regions for optimal generation of CTL epitopes
authorSteers, Nicholas J ; Currier, Jeffrey R ; Jobe, Ousman ; Tovanabutra, Sodsai ; Ratto-Kim, Silvia ; Marovich, Mary A ; Kim, Jerome H ; Michael, Nelson L ; Alving, Carl R ; Rao, Mangala
creationdate20140612
lso0120140612
facets
frbrgroupid6627143059925563657
frbrtype5
newrecords20190904
languageeng
topic
0HIV-1
1Gag-P24
2Proteasome
3Ctl-Epitope
4Synthetic Flanking Region
5Immunogen Design
6Medicine
7Biology
8Veterinary Medicine
9Pharmacy, Therapeutics, & Pharmacology
collectionScienceDirect (Elsevier)
prefilterarticles
rsrctypearticles
creatorcontrib
0Steers, Nicholas J
1Currier, Jeffrey R
2Jobe, Ousman
3Tovanabutra, Sodsai
4Ratto-Kim, Silvia
5Marovich, Mary A
6Kim, Jerome H
7Michael, Nelson L
8Alving, Carl R
9Rao, Mangala
jtitleVaccine
creationdate2014
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Steers
1Currier
2Jobe
3Tovanabutra
4Ratto-Kim
5Marovich
6Kim
7Michael
8Alving
9Rao
aufirst
0Nicholas J
1Jeffrey R
2Ousman
3Sodsai
4Silvia
5Mary A
6Jerome H
7Nelson L
8Carl R
9Mangala
auinitN
auinit1N
au
0Steers, Nicholas J
1Currier, Jeffrey R
2Jobe, Ousman
3Tovanabutra, Sodsai
4Ratto-Kim, Silvia
5Marovich, Mary A
6Kim, Jerome H
7Michael, Nelson L
8Alving, Carl R
9Rao, Mangala
atitleDesigning the epitope flanking regions for optimal generation of CTL epitopes
jtitleVaccine
risdate20140612
volume32
issue28
spage3509
epage3516
pages3509-3516
issn0264-410X
eissn1873-2518
formatjournal
genrearticle
ristypeJOUR
abstract

The flanking amino acids that surround epitopes are critical for effective antigen processing and maintenance of epitope integrity. In the present study, the frequency and characteristics of each amino acid that flanked the peptides generated from the proteasomal degradation of three different subtypes of HIV-1 Gag-p24 were determined. Synthetic flanking regions were designed based on the highest and the lowest frequencies of amino acid with the ideal characteristics at positions upstream and downstream of the proteasomal cleavage site. Peptides were synthesized that contained known CD8+ CTL-epitopes from HIV-1 Gag, CMV pp65, and vaccinia proteins HRP-2, and C16, flanked by amino acid sequences specifically designed to either generate or inhibit the known CD8+ CTL-epitopes. As predicted, the known CD8+ CTL-epitopes were effectively generated from the peptides with synthetic flanking regions specifically designed to promote epitope generation in a proteasome-dependent manner. The...

pubElsevier Ltd
doi10.1016/j.vaccine.2014.04.039
lad01Vaccine
date2014-06-12