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Pulmonary dendritic cells isolated from neonatal and adult ovine lung tissue

Lung dendritic cells (DCs) are potent antigen presenting cells (APCs) that initiate and modulate the adaptive immune response upon microbial infection within the pulmonary environment. For the first time, neonatal and adult lung DCs in a large animal model were compared in these studies. Here, we is... Full description

Journal Title: Veterinary Immunology and Immunopathology 2006, Vol.112(3), pp.171-182
Main Author: Fach, Sasha J
Other Authors: Brockmeier, Susan L , Hobbs, Lea Ann , Lehmkuhl, Howard D , Sacco, Randy E
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0165-2427 ; E-ISSN: 1873-2534 ; DOI: 10.1016/j.vetimm.2006.02.012
Link: https://www.sciencedirect.com/science/article/pii/S016524270600064X
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recordid: elsevier_sdoi_10_1016_j_vetimm_2006_02_012
title: Pulmonary dendritic cells isolated from neonatal and adult ovine lung tissue
format: Article
creator:
  • Fach, Sasha J
  • Brockmeier, Susan L
  • Hobbs, Lea Ann
  • Lehmkuhl, Howard D
  • Sacco, Randy E
subjects:
  • Ovine Lung
  • Dendritic Cells
  • Neonates
  • Medicine
  • Veterinary Medicine
ispartof: Veterinary Immunology and Immunopathology, 2006, Vol.112(3), pp.171-182
description: Lung dendritic cells (DCs) are potent antigen presenting cells (APCs) that initiate and modulate the adaptive immune response upon microbial infection within the pulmonary environment. For the first time, neonatal and adult lung DCs in a large animal model were compared in these studies. Here, we isolated and identified lung DCs in both neonatal and adult sheep, a valuable experimental animal utilized in pulmonary studies of naturally occurring respiratory diseases. Neonatal lung DCs exhibited characteristic dendrites and morphology when observed by transmission electron microscopy and expressed low to moderate DEC-205, CD80/86, MHC class II and CD 14. Regardless of age, lung DCs were functionally able to endocytose FITC conjugated ovalbumin but to a lesser degree than monocyte-derived DCs. In addition, neonatal lung DCs were demonstrated to be potent stimulators of allogeneic T cell proliferation. Together, these results demonstrate that neonatal and adult lung DCs are functionally...
language: eng
source:
identifier: ISSN: 0165-2427 ; E-ISSN: 1873-2534 ; DOI: 10.1016/j.vetimm.2006.02.012
fulltext: fulltext
issn:
  • 0165-2427
  • 01652427
  • 1873-2534
  • 18732534
url: Link


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subjectOvine Lung ; Dendritic Cells ; Neonates ; Medicine ; Veterinary Medicine
descriptionLung dendritic cells (DCs) are potent antigen presenting cells (APCs) that initiate and modulate the adaptive immune response upon microbial infection within the pulmonary environment. For the first time, neonatal and adult lung DCs in a large animal model were compared in these studies. Here, we isolated and identified lung DCs in both neonatal and adult sheep, a valuable experimental animal utilized in pulmonary studies of naturally occurring respiratory diseases. Neonatal lung DCs exhibited characteristic dendrites and morphology when observed by transmission electron microscopy and expressed low to moderate DEC-205, CD80/86, MHC class II and CD 14. Regardless of age, lung DCs were functionally able to endocytose FITC conjugated ovalbumin but to a lesser degree than monocyte-derived DCs. In addition, neonatal lung DCs were demonstrated to be potent stimulators of allogeneic T cell proliferation. Together, these results demonstrate that neonatal and adult lung DCs are functionally...
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Lung dendritic cells (DCs) are potent antigen presenting cells (APCs) that initiate and modulate the adaptive immune response upon microbial infection within the pulmonary environment. For the first time, neonatal and adult lung DCs in a large animal model were compared in these studies. Here, we isolated and identified lung DCs in both neonatal and adult sheep, a valuable experimental animal utilized in pulmonary studies of naturally occurring respiratory diseases. Neonatal lung DCs exhibited characteristic dendrites and morphology when observed by transmission electron microscopy and expressed low to moderate DEC-205, CD80/86, MHC class II and CD 14. Regardless of age, lung DCs were functionally able to endocytose FITC conjugated ovalbumin but to a lesser degree than monocyte-derived DCs. In addition, neonatal lung DCs were demonstrated to be potent stimulators of allogeneic T cell proliferation. Together, these results demonstrate that neonatal and adult lung DCs are functionally...

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Lung dendritic cells (DCs) are potent antigen presenting cells (APCs) that initiate and modulate the adaptive immune response upon microbial infection within the pulmonary environment. For the first time, neonatal and adult lung DCs in a large animal model were compared in these studies. Here, we isolated and identified lung DCs in both neonatal and adult sheep, a valuable experimental animal utilized in pulmonary studies of naturally occurring respiratory diseases. Neonatal lung DCs exhibited characteristic dendrites and morphology when observed by transmission electron microscopy and expressed low to moderate DEC-205, CD80/86, MHC class II and CD 14. Regardless of age, lung DCs were functionally able to endocytose FITC conjugated ovalbumin but to a lesser degree than monocyte-derived DCs. In addition, neonatal lung DCs were demonstrated to be potent stimulators of allogeneic T cell proliferation. Together, these results demonstrate that neonatal and adult lung DCs are functionally...

pubElsevier B.V
doi10.1016/j.vetimm.2006.02.012
lad01Veterinary Immunology and Immunopathology
date2006-08-15