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Prognostic Significance of Gremlin1 ( GREM1) Promoter CpG Island Hypermethylation in Clear Cell Renal Cell Carcinoma

Gremlin1 ( GR1 ), a bone morphogenetic protein antagonist and putative angiogenesis-modulating gene, is silenced by promoter hypermethylation in human malignancies. Here we study GR1 methylation in clear cell renal cell carcinoma (ccRCC) and its impact on tumor characteristics and clinical outcome.... Full description

Journal Title: The American Journal of Pathology 2010, Vol.176(2), pp.575-584
Main Author: van Vlodrop, Iris J.H
Other Authors: Baldewijns, Marcella M.L , Smits, Kim M , Schouten, Leo J , van Neste, Leander , van Criekinge, Wim , van Poppel, Hein , Lerut, Evelyne , Schuebel, Kornel E , Ahuja, Nita , Herman, James G , de Bruïne, Adriaan P , van Engeland, Manon
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0002-9440 ; E-ISSN: 1525-2191 ; DOI: 10.2353/ajpath.2010.090442
Link: http://dx.doi.org/10.2353/ajpath.2010.090442
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recordid: elsevier_sdoi_10_2353_ajpath_2010_090442
title: Prognostic Significance of Gremlin1 ( GREM1) Promoter CpG Island Hypermethylation in Clear Cell Renal Cell Carcinoma
format: Article
creator:
  • van Vlodrop, Iris J.H
  • Baldewijns, Marcella M.L
  • Smits, Kim M
  • Schouten, Leo J
  • van Neste, Leander
  • van Criekinge, Wim
  • van Poppel, Hein
  • Lerut, Evelyne
  • Schuebel, Kornel E
  • Ahuja, Nita
  • Herman, James G
  • de Bruïne, Adriaan P
  • van Engeland, Manon
subjects:
  • Medicine
ispartof: The American Journal of Pathology, 2010, Vol.176(2), pp.575-584
description: Gremlin1 ( GR1 ), a bone morphogenetic protein antagonist and putative angiogenesis-modulating gene, is silenced by promoter hypermethylation in human malignancies. Here we study GR1 methylation in clear cell renal cell carcinoma (ccRCC) and its impact on tumor characteristics and clinical outcome. Three GR1 promoter CpG island regions (i, ii, iii) were analyzed by methylation-specific PCR and/or bisulfite sequencing in ccRCC cell lines and ccRCCs from two independent patient series. Results were correlated with clinicopathological and angiogenic parameters. Bisulfite sequencing of ccRCC cell lines showed GR1 methylation, associated with absence of GR1 mRNA. GR1 methylation prevalence in ccRCCs varied between regions: 55%, 24%, and 20% for regions i, ii, and iii, respectively. GR1 region iii methylation was associated with increased tumor size ( P = 0.02), stage ( ...
language: eng
source:
identifier: ISSN: 0002-9440 ; E-ISSN: 1525-2191 ; DOI: 10.2353/ajpath.2010.090442
fulltext: fulltext
issn:
  • 0002-9440
  • 00029440
  • 1525-2191
  • 15252191
url: Link


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titlePrognostic Significance of Gremlin1 ( GREM1) Promoter CpG Island Hypermethylation in Clear Cell Renal Cell Carcinoma
creatorvan Vlodrop, Iris J.H ; Baldewijns, Marcella M.L ; Smits, Kim M ; Schouten, Leo J ; van Neste, Leander ; van Criekinge, Wim ; van Poppel, Hein ; Lerut, Evelyne ; Schuebel, Kornel E ; Ahuja, Nita ; Herman, James G ; de Bruïne, Adriaan P ; van Engeland, Manon
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descriptionGremlin1 ( GR1 ), a bone morphogenetic protein antagonist and putative angiogenesis-modulating gene, is silenced by promoter hypermethylation in human malignancies. Here we study GR1 methylation in clear cell renal cell carcinoma (ccRCC) and its impact on tumor characteristics and clinical outcome. Three GR1 promoter CpG island regions (i, ii, iii) were analyzed by methylation-specific PCR and/or bisulfite sequencing in ccRCC cell lines and ccRCCs from two independent patient series. Results were correlated with clinicopathological and angiogenic parameters. Bisulfite sequencing of ccRCC cell lines showed GR1 methylation, associated with absence of GR1 mRNA. GR1 methylation prevalence in ccRCCs varied between regions: 55%, 24%, and 20% for regions i, ii, and iii, respectively. GR1 region iii methylation was associated with increased tumor size ( P = 0.02), stage ( ...
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titlePrognostic Significance of Gremlin1 ( GREM1) Promoter CpG Island Hypermethylation in Clear Cell Renal Cell Carcinoma
description

Gremlin1 ( GREM1 ), a bone morphogenetic protein antagonist and putative angiogenesis-modulating gene, is silenced by promoter hypermethylation in human malignancies. Here we study GREM1 methylation in clear cell renal cell carcinoma (ccRCC) and its impact on tumor characteristics and clinical outcome. Three GREM1 promoter CpG island regions (i, ii, iii) were analyzed by methylation-specific PCR and/or bisulfite sequencing in ccRCC cell lines and ccRCCs from two independent patient series. Results were correlated with clinicopathological and angiogenic parameters. Bisulfite sequencing of ccRCC cell lines showed GREM1 methylation, associated with absence of GREM1 mRNA. GREM1 methylation prevalence in ccRCCs varied between regions: 55%, 24%, and 20% for regions i, ii, and iii, respectively. GREM1 region iii methylation was associated with increased tumor size ( P = 0.02), stage ( ...

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atitlePrognostic Significance of Gremlin1 ( GREM1) Promoter CpG Island Hypermethylation in Clear Cell Renal Cell Carcinoma
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abstract

Gremlin1 ( GREM1 ), a bone morphogenetic protein antagonist and putative angiogenesis-modulating gene, is silenced by promoter hypermethylation in human malignancies. Here we study GREM1 methylation in clear cell renal cell carcinoma (ccRCC) and its impact on tumor characteristics and clinical outcome. Three GREM1 promoter CpG island regions (i, ii, iii) were analyzed by methylation-specific PCR and/or bisulfite sequencing in ccRCC cell lines and ccRCCs from two independent patient series. Results were correlated with clinicopathological and angiogenic parameters. Bisulfite sequencing of ccRCC cell lines showed GREM1 methylation, associated with absence of GREM1 mRNA. GREM1 methylation prevalence in ccRCCs varied between regions: 55%, 24%, and 20% for regions i, ii, and iii, respectively. GREM1 region iii methylation was associated with increased tumor size ( P = 0.02), stage ( ...

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doi10.2353/ajpath.2010.090442
lad01The American Journal of Pathology